重组Anti-Albumin抗体[EPR20195] (ab207327)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20195] to Albumin
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Albumin抗体[EPR20195]
参阅全部 Albumin 一抗 -
描述
兔单克隆抗体[EPR20195] to Albumin -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length native protein (purified) corresponding to Human Albumin aa 1 to the C-terminus. (Purified Proteins from Normal Serum).
Database link: P02768 -
阳性对照
- WB: Human, mouse and rat liver and plasma lysates. Human serum lysates. Human fetal kidney and spleen lysates. Mouse and rat spleen and kidney lysates. HepG2, NIH/3T3 and PC-12 whole cell lysates. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20195 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab207327于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/500.
Permeabilization is required. |
|
WB |
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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IP |
1/40.
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ICC/IF |
1/500.
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说明 |
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Flow Cyt (Intra)
1/500. Permeabilization is required. |
WB
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
IP
1/40. |
ICC/IF
1/500. |
靶标
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 213 Human
- Entrez Gene: 11657 Mouse
- Entrez Gene: 24186 Rat
- Omim: 103600 Human
- SwissProt: P02768 Human
- SwissProt: P07724 Mouse
- SwissProt: P02770 Rat
- Unigene: 418167 Human
see all -
形式
There are 2 isoforms produced by alternative splicing. -
别名
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
图片
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All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution
Lane 1 : Human liver lysate at 20 µg
Lane 2 : Mouse liver lysate at 20 µg
Lane 3 : Rat liver lysate at 20 µg
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-3: 5 seconds; Lane 4/5: 1 second.
Negative control: HeLa (PMID: 10476216 and 8314088).
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Lanes 1-4 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/10000 dilution
Lanes 5-6 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution
Lane 1 : Human plasma at 2 µg
Lane 2 : Rat plasma at 2 µg
Lane 3 : Mouse plasma at 2 µg
Lane 4 : Human serum at 2 µg
Lane 5 : Human fetal kidney lysate at 10 µg
Lane 6 : Human fetal spleen lysate at 10 µg
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : Rat kidney lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 1 second; Lane 5: 3 minutes; Lane 6: 5 seconds.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HepG2 cell line.
Negative control: HeLa (PMID: 10476216 and 8314088)).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albuminwith ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
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Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10µg (Input).
Lane 2: ab207327 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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Albumin antibody ab207327 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D hepatocyte cell culture.
Blue: DAPI, Green: CD68, Yellow: Albumin, Red: Vimentin
Learn more about 3D cell culture and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (62)
ab207327 被引用在 62 文献中.
- Zheng R et al. Fine Particulate Matter Induces Childhood Asthma Attacks via Extracellular Vesicle-Packaged Let-7i-5p-Mediated Modulation of the MAPK Signaling Pathway. Adv Sci (Weinh) 9:e2102460 (2022). PubMed: 34816611
- Liu Q et al. ECM1 modified HF-MSCs targeting HSC attenuate liver cirrhosis by inhibiting the TGF-β/Smad signaling pathway. Cell Death Discov 8:51 (2022). PubMed: 35136027
- Yuan S et al. SOX8 Affects Tumoral SPARC Expression by Regulating EZH2 to Attenuate Effectiveness of albumin-bound paclitaxel in PDAC. Int J Biol Sci 18:911-922 (2022). PubMed: 35173526
- Wang DH et al. Isolation and characterization mesenchymal stem cells from red panda (Ailurus fulgens styani) endometrium. Conserv Physiol 10:coac004 (2022). PubMed: 35211318
- Tu S et al. Icaritin ameliorates extracellular microparticles-induced inflammatory pre-metastatic niche via modulating the cGAS-STING signaling. Phytother Res 36:2127-2142 (2022). PubMed: 35257426