AB207327

Anti-Albumin 抗体 [EPR20195]

Name: Albumin抗体[EPR20195] (ab207327)| Abcam中文官网
Brand: Abcam Limited
SKU: AB207327
Price: 3352 CNY
Availability: InStock
Rating: 5 (2 reviews)

Anti-Albumin antibody [EPR20195]

RabMAb
Recombinant
Lab Essentials
了解详情

(2 Reviews)

(126 Publications)

Anti-Albumin antibody [EPR20195] (ab207327) is a rabbit monoclonal antibody detecting Albumin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 80 publications

查看别名

GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341, ALB, Albumin

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

Albumin antibody ab207327 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D hepatocyte cell culture. Blue : DAPI, Green : CD68, Yellow : Albumin, Red : Vimentin Learn more about 3D cell culture and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section.

Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] (AB207327)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] (AB207327)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albuminwith ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr^® 488) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (AB207327)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on HepG2 cell line.

Negative control : HeLa (PMID : 10476216 and 8314088)).

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Supplier Data

Western blot - Anti-Albumin antibody [EPR20195] (AB207327)

Blocking/Dilution buffer : 5% NFDM/TBST.

Lanes 1 - 4:

Western blot - Anti-Albumin antibody [EPR20195] (ab207327) at 1/10000 dilution

Lanes 5 - 6:

Western blot - Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

Lane 1:

Human plasma at 2 µg

Lane 2:

Rat plasma at 2 µg

Lane 3:

Mouse plasma at 2 µg

Lane 4:

Human serum at 2 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Lane 6:

Human fetal spleen lysate at 10 µg

Secondary

Lanes 1 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 5 - 6:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-Albumin antibody [EPR20195] (AB207327)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-3 : 5 seconds; Lane 4/5 : 1 second.

Negative control : HeLa (PMID : 10476216 and 8314088).

All lanes:

Western blot - Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

Lane 1:

Human liver lysate at 20 µg

Lane 2:

Mouse liver lysate at 20 µg

Lane 3:

Rat liver lysate at 20 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Supplier Data

Immunoprecipitation - Anti-Albumin antibody [EPR20195] (AB207327)

Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HepG2 whole cell lysate, 10μg (Input).

Lane 2 : ab207327 IP in HepG2 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Albumin antibody [EPR20195] (ab207327)

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Supplier Data

Western blot - Anti-Albumin antibody [EPR20195] (AB207327)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-4 : 1 second; Lane 5 : 3 minutes; Lane 6 : 5 seconds.

All lanes:

Western blot - Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

Lane 1:

Mouse spleen lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Rat spleen lysate at 10 µg

Lane 4:

Rat kidney lysate at 10 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20195

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, Flow Cyt (Intra), ICC/IF, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "Permeabilization is required." }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Anti-Albumin antibody [EPR20195] (ab207327) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IP, WB in human, mouse, rat samples.

Anti-Albumin antibody (UNQ696/PRO1341 antibody) [EPR20195] (ab207327) has been cited over 82 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Albumin antibody [EPR20195] (ab207327) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Albumin antibody [EPR20195] (ab207327) specifically detects Albumin (UniProt ID: P02768; Molecular weight: 66kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR20195 - ab271979.

Antibody clone EPR20195 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (ab313348).

Albumin (UNQ696/PRO1341), a crucial blood protein produced by the liver, plays a vital role in maintaining fluid balance and transporting various substances throughout the body. Abnormal serum albumin levels can indicate liver function issues, kidney disease, or nutritional deficiencies. Conditions like hypoalbuminemia and hyperalbuminemia are linked to diseases such as diabetes, cancer, and cardiovascular disease. Our Alb antibodyis a valuable tool in research, aiding in the detection and study of albumin-related pathways. Understanding albumin's role in key pathways, including its antioxidant properties and involvement in osmotic regulation, is essential for diagnosing and managing these health conditions.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed : 19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc > calcium > magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed : 6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed : 6234017).

See full target information ALB

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文献 (126)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 15:1561806 PubMed40661967

2025

CD8 T cells promote tubule-interstitial damage in malaria-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas Esteves Teixeira,Sarah Aparecida Dos Santos Alves,Alessandro Sá Pinheiro,Leandro Souza Silva,Rodrigo Pacheco Silva-Aguiar,Diogo Barros Peruchetti,Tatiana Almeida Pádua,Mariana Conceição Souza,Celso Caruso-Neves,Ana Acacia Sá Pinheiro

Pediatric discovery.. 2:e2500 PubMed40625459

2025

Astrocytic connexin 43-hemichannels aggravate seizures by modulating blood-brain barrier permeability in temporal lobe epilepsy mice.

Applications

Unspecified application

Species

Unspecified reactive species

Honghong Song,Yi Li,Jiayu Liu,Xianshu Bai,Li Jiang,Tingsong Li

Iranian journal of basic medical sciences 28:1004-1011 PubMed40584448

2025

Inhibition of HDAC3 induces neuroprotection by activating the Npas4 signaling pathway following surgical brain injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Haiping Gu,Yating Gong,Muyao Wu,Mengying Shi,Jiejie Yu,Hongfei Zhu,Ya-Ming Sun,Baoqi Dang

Materials today. Bio 32:101824 PubMed40487153

2025

Bioprinting of hepatic tissue model using photocrosslinkable dECM-containing composite hydrogel.

Applications

Unspecified application

Species

Unspecified reactive species

Nima Tabatabaei Rezaei,Hitendra Kumar,Hongqun Liu,Ashna Rajeev,Giovanniantonio Natale,Samuel S Lee,Simon S Park,Keekyoung Kim

Regenerative therapy 30:1-8 PubMed40476159

2025

Transplantation of chemically induced liver progenitors in Nagase analbuminemic rats under liver regenerative stimulus.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Fukumoto,Akihiko Soyama,Daisuke Miyamoto,Takanobu Hara,Hajime Matsushima,Hajime Imamura,Mampei Yamashita,Tomohiko Adachi,Kengo Kanetaka,Susumu Eguchi

International journal of molecular sciences 26: PubMed40429837

2025

Biosynthesized Calcium Peroxide Nanoparticles as a Multifunctional Platform for Liver Cancer Therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Wu,Siqi Li,Xin Xia,Gen Zhang,Ting Wang

Frontiers in medicine 12:1529495 PubMed40421300

2025

Proteomic insights into COPD pathogenesis and therapeutic targets: a causal analysis of circulating proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Min Luo,Ying Tang,Jing Qiu,Kai Yang,WenJing Dai,EnRong Fang

Functional & integrative genomics 25:90 PubMed40240625

2025

Identification and mechanistic analysis of shared biomarkers and pathogenesis in acute pancreatitis and sepsis based on differential gene expression and protein interaction networks.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Lu,Yifeng Mao,Shangwen Cai,Qingqing Chen,Panpan Xu,Chenghua Xu,Cheng Zheng,Jian Lan

Materials today. Bio 32:101700 PubMed40225139

2025

Engineering a dynamic extracellular matrix using thrombospondin-1 to propel hepatocyte organoids reprogramming and improve mouse liver regeneration post-transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Yan Xu,Min Wang,Jing-Yan Shi,Ye Liu,Chao Yu,Xin-Yi Zhang,Chen-Wei Zhang,Qi-Feng He,Chao Pan,Jin Zhou,Hua Xiao,Hong-Yong Cao,Yong Ma

Nature communications 16:1736 PubMed39966385

2025

A glucose-enriched lung pre-metastatic niche triggered by matrix stiffness-tuned exosomal miRNAs in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Zhao,Hongmei Yu,Jiajun Li,Jiali Qian,Miao Li,Xi Zhang,Mimi Wang,Yaohui Wang,Yinying Dong,Yang You,Qiwen Zhou,Dongmei Gao,Yan Zhao,Binbin Liu,Rongxin Chen,Zhenggang Ren,Zhiming Wang,Kezhi Zhang,Jiefeng Cui

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