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重组RabMAb

重组Anti-Albumin抗体[EPR12780(B)] (ab175934)

Submit a review Submit a question References (2)
Western blot - Anti-Albumin antibody [EPR12780(B)] (ab175934)
  • Anti-Albumin antibody [EPR12780(B)] (ab175934)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12780(B)] to Albumin
  • Suitable for: WB
  • Reacts with: Cow

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Albumin抗体[EPR12780(B)]
    参阅全部 Albumin 一抗

  • 描述

    兔单克隆抗体[EPR12780(B)] to Albumin

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Cow

  • 免疫原

    Synthetic peptide within Cow Albumin aa 500-600 (Cysteine residue). The exact sequence is proprietary.
    Database link: P02769

  • 阳性对照

    • WB: Bovine serum albumin lysate

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175934于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
说明
WB
1/1000 - 1/5000. Predicted molecular weight: 69 kDa.
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

靶标

  • 功能

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

  • 组织特异性

    Plasma.

  • 疾病相关

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.

  • 序列相似性

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • 翻译后修饰

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 形式

    There are 2 isoforms produced by alternative splicing.

  • 别名

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • GIG20 antibody
    • GIG42 antibody
    • Growth inhibiting protein 20 antibody
    • growth-inhibiting protein 20 antibody
    • OTTHUMP00000220436 antibody
    • OTTHUMP00000220438 antibody
    • OTTHUMP00000220439 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • PRO1708 antibody
    • PRO2044 antibody
    • PRO2619 antibody
    • PRO2675 antibody
    • Serum albumin antibody
    • UNQ696/PRO1341 antibody
    see all

图片

  • Western blot - Anti-Albumin antibody [EPR12780(B)] (ab175934)
    Western blot - Anti-Albumin antibody [EPR12780(B)] (ab175934)
    Anti-Albumin antibody [EPR12780(B)] (ab175934) at 1/1000 dilution + Bovine serum albumin lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  • Anti-Albumin antibody [EPR12780(B)] (ab175934)
    Anti-Albumin antibody [EPR12780(B)] (ab175934)

文献 (2)

发表研究结果有使用 ab175934？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab175934 被引用在 2 文献中.

  • Visan KS  et al. Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles. J Extracell Vesicles 11:e12266 (2022). PubMed: 36124834
  • Martínez-Greene JA  et al. Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer-based precipitation and size exclusion chromatography. J Extracell Vesicles 10:e12087 (2021). PubMed: 33936570

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