重组Anti-AKT3 + AKT1抗体[E45] (ab32038)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.cn/ps/products/32/ab32038/Images/ab32038-43706-anti-akt3-akt1-antibody-e45-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E45] to AKT3 + AKT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-AKT3 + AKT1抗体[E45] -
描述
兔单克隆抗体[E45] to AKT3 + AKT1 -
宿主
Rabbit -
特异性
This antibody detects Akt1 and Akt3.
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经测试应用
适用于: IHC-P, WB, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Synthetic peptide corresponding to Human AKT1 aa 450-550.
Database link: P31749 -
阳性对照
- Squamous lung carcinoma ICC/IF: SK-BR-3 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E45 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32038于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration.
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| ICC/IF |
1/500.
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| 说明 |
|---|
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IHC-P
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. |
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ICC/IF
1/500. |
靶标
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细胞定位
AKT3: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. AKT1: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. -
数据库链接
- Entrez Gene: 100137872 Cow
- Entrez Gene: 280991 Cow
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 23797 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 29414 Rat
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)
Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.cn/ps/products/32/ab32038/Images/ab32038-4-anti-akt3-akt1-antibody-e45-immunocytochemistry-skbr3-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] (ab32038)Immunocytochemistry/ Immunofluorescence analysis of SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling AKT3+AKT1 with purified ab32038 at 1/500 dilution (2.1 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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![Western blot - Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.cn/ps/products/32/ab32038/Images/ab32038-267109-anti-akt3-akt1-antibody-e45-western-blot.jpg)
Western blot - Anti-AKT3 + AKT1 antibody [E45] (ab32038)All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution
Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
Lanes 2 & 4 & 6 : Empty vector transfected 293T cell lysate
Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Exposure time: 1 secondBlocking/Diluting buffer: 5% NFDM/TBST.
Observed band: 82kDa, 56kDa.
82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT.
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![Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.cn/ps/products/32/ab32038/Images/ab32038-5-benefits-of-recombinant-antibodies.png)
Anti-AKT3 + AKT1 antibody [E45] (ab32038)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab32038 被引用在 11 文献中.
- Qiu Q et al. HDAC I/IIb selective inhibitor Purinostat Mesylate combined with GLS1 inhibition effectively eliminates CML stem cells. Bioact Mater 21:483-498 (2023). PubMed: 36185739
- Li N et al. Cross-talk between TNF-a and IFN-? signaling in induction of B7-H1 expression in hepatocellular carcinoma cells. Cancer Immunol Immunother 67:271-283 (2018). PubMed: 29090321
- Yang CY et al. Melatonin exerts anti-oral cancer effect via suppressing LSD1 in patient-derived tumor xenograft models. Oncotarget 8:33756-33769 (2017). PubMed: 28422711
- Liao P et al. Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis. Elife 6:N/A (2017). WB . PubMed: 28440748
- Chen YC et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep 6:18815 (2016). WB ; Human . PubMed: 26739898