Anti-AKT2 (phospho S474)抗体(ab38513)
Key features and details
- Rabbit polyclonal to AKT2 (phospho S474)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AKT2 (phospho S474)抗体
参阅全部 AKT2 一抗 -
描述
兔多克隆抗体to AKT2 (phospho S474) -
宿主
Rabbit -
特异性
Akt2 (phospho-Ser474) antibody detects endogenous levels of Akt2 only when phosphorylated at serine 474. The region of AKT2 surrounding S474 has a high degree of similarity to the corresponding regions in AKT1 and AKT3 and thus may cross react with these proteins if phosphorylated on the corresponding serine residue. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human AKT2 aa 432-481 (C terminal) (phospho S474). The exact sequence is proprietary. The antiserum was produced against synthesized phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474.
Sequence:SEVDTRYFD DEFTAQSITI TPPDRYDSLG LLELDQRTHF PQFSYSASIR E
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with Recombinant human AKT2 protein (ab79798).
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| IHC-P | (2) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with Recombinant human AKT2 protein (ab79798). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
General protein kinase capable of phosphorylating several known proteins. -
组织特异性
Expressed in all human cell types so far analyzed. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
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数据库链接
- Entrez Gene: 208 Human
- Omim: 164731 Human
- SwissProt: P31751 Human
- Unigene: 631535 Human
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别名
- AKT antibody
- Akt2 antibody
- AKT2_HUMAN antibody
see all
图片
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Western blot - Anti-AKT2 (phospho S474) antibody (ab38513)All lanes : Anti-AKT2 (phospho S474) antibody (ab38513)
Lane 1 : 293 cells treated for 30 min with 200ng/ml EGF
Lane 2 : 293 cells treated for 30 min with 200ng/ml EGF with Phospho peptide
Predicted band size: 55 kDa -
Western blot - Anti-AKT2 (phospho S474) antibody (ab38513)All lanes : Anti-AKT2 (phospho S474) antibody (ab38513) at 1/500 dilution
Lane 1 : Extract of A2780 cells.
Lane 2 : Extract of A2780 cells + TNF alpha.
Lane 3 : Extract of A2780 cells + UV.
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Lanes can be loaded with 5-30µg of total protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT2 (phospho S474) antibody (ab38513)This image shows human lung carcinoma tissue stained with ab38513 at 1/50 dilution.
Image on left: untreated.
Image on right: treated with phosphopeptide at 1µg/ml (negative control).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT2 (phospho S474) antibody (ab38513)This image is courtesy of an anonymous Abreviewab38513 staining AKT2 in human infantile fibromatosis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 3 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/150 in TBS + 1% BSA + 1% FBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab38513 被引用在 25 文献中.
- Wang Y et al. Inhibition of bone morphogenetic protein receptor 2 suppresses pancreatic ductal adenocarcinoma growth by regulating GRB2/PI3K/AKT axis. Ann Transl Med 9:557 (2021). PubMed: 33987255
- Li M et al. MicroRNA-29b participates in the epithelial-mesenchymal transition of retinal pigment epithelial cells through p-p65. Exp Ther Med 22:868 (2021). PubMed: 34194546
- Zhou L et al. Herbal complex 'Buyang Huanwu Tang' improves motor endplate function of denervated-dependent skeletal muscle atrophy in rat. J Integr Neurosci 19:89-99 (2020). PubMed: 32259889
- Hayashi-Hori M et al. Therapeutic Effect of Rapamycin on Aortic Dissection in Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32397282
- Zhao J et al. Metoprolol alleviates arginine vasopressin-induced cardiomyocyte hypertrophy by upregulating the AKT1-SERCA2 cascade in H9C2 cells. Cell Biosci 10:72 (2020). PubMed: 32489586