重组Anti-AKT2抗体[EP1676] (ab131168)
![Western blot - Anti-AKT2 antibody [EP1676] (ab131168)](https://www.abcam.cn/ps/products/131/ab131168/Images/ab131168-535113-anti-akt2-antibody-ep1676-western-blot-human-mouse-rat-jurkat-jar-nih3t3-c6.jpg "Western blot - Anti-AKT2 antibody [EP1676] (ab131168)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1676] to AKT2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-AKT2抗体[EP1676]
参阅全部 AKT2 一抗 -
描述
兔单克隆抗体[EP1676] to AKT2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Jurkat, JAR, NIH/3T3, and C6 cell lysates.
-
常规说明
This product has switched from a hybridoma to recombinant production method on 9th February 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.20 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1676 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131168于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
|
| 说明 |
|---|
|
WB
1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
-
功能
General protein kinase capable of phosphorylating several known proteins. -
组织特异性
Expressed in all human cell types so far analyzed. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
-
数据库链接
- Entrez Gene: 208 Human
- Entrez Gene: 11652 Mouse
- Entrez Gene: 25233 Rat
- Omim: 164731 Human
- SwissProt: P31751 Human
- SwissProt: Q60823 Mouse
- SwissProt: P47197 Rat
- Unigene: 631535 Human
see all -
别名
- AKT antibody
- Akt2 antibody
- AKT2_HUMAN antibody
see all
图片
-
Western blot - Anti-AKT2 antibody [EP1676] (ab131168)All lanes : Anti-AKT2 antibody [EP1676] (ab131168) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Anti-AKT2 antibody [EP1676] (ab131168)](https://www.abcam.cn/ps/products/131/ab131168/Images/ab131168-4-benefits-of-recombinant-antibodies.png)
Anti-AKT2 antibody [EP1676] (ab131168)
数据表及文件
-
SDS download
-
Datasheet download
文献 (9)
ab131168 被引用在 9 文献中.
- Yang Y et al. Anti-Inflammatory Effects of Allocryptopine via the Target on the CX3CL1-CX3CR1 axis/GNB5/AKT/NF-κB/Apoptosis in Dextran Sulfate-Induced Mice. Biomedicines 11:N/A (2023). PubMed: 36831001
- Ni Q et al. Exosomal lncRNA HCG18 contributes to cholangiocarcinoma growth and metastasis through mediating miR-424-5p/SOX9 axis through PI3K/AKT pathway. Cancer Gene Ther 30:582-595 (2023). PubMed: 36854894
- Wu C et al. Interaction between the PI3K/AKT pathway and mitochondrial autophagy in macrophages and the leukocyte count in rats with LPS-induced pulmonary infection. Open Life Sci 18:20220588 (2023). PubMed: 37077346
- Geng X et al. BDNF alleviates Parkinson's disease by promoting STAT3 phosphorylation and regulating neuronal autophagy. Cell Tissue Res 393:455-470 (2023). PubMed: 37450039
- Li M et al. MicroRNA-29b participates in the epithelial-mesenchymal transition of retinal pigment epithelial cells through p-p65. Exp Ther Med 22:868 (2021). PubMed: 34194546
- Zhang H et al. Inhibition of integrin subunit alpha 11 restrains gastric cancer progression through phosphatidylinositol 3-kinase/Akt pathway. Bioengineered 12:11909-11921 (2021). PubMed: 34802381
- Zeng J et al. Hsp90 up-regulates PD-L1 to promote HPV-positive cervical cancer via HER2/PI3K/AKT pathway. Mol Med 27:130 (2021). PubMed: 34666670
- Xu X et al. Triptolide inhibits angiogenesis in microvascular endothelial cells through regulation of miR-92a. J Physiol Biochem 75:573-583 (2019). PubMed: 31691162
- Duan C et al. Sulfasalazine alters microglia phenotype by competing endogenous RNA effect of miR-136-5p and long non-coding RNA HOTAIR in cuprizone-induced demyelination. Biochem Pharmacol 155:110-123 (2018). PubMed: 29944870