重组Anti-AKT1 + AKT2抗体[EPR17062] (ab182729)
![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264336-182729WB1.jpg "Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17062] to AKT1 + AKT2
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
概述
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产品名称
Anti-AKT1 + AKT2抗体[EPR17062]
参阅全部 AKT1 + AKT2 一抗 -
描述
兔单克隆抗体[EPR17062] to AKT1 + AKT2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human, Recombinant fragment -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human AKT1 fragment recombinant protein; Human AKT2 fragment recombinant protein; Human fetal heart and fetal kidney lysates; HeLa, MCF7, C6, RAW 264.7 and NIH/3T3 whole cell lysates; Mouse and rat brain and heart lysates. IHC-P: Human tonsil, Human bladder cancer, mouse stomach and rat cerebrum tissues. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: HeLa whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17062 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182729于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
|
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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| ICC/IF |
1/500.
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| IP |
1/40.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/600. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
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ICC/IF
1/500. |
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IP
1/40. |
靶标
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相关性
The serine/threonine kinase AKT (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "AKT family": AKT1, AKT2, and AKT3. AKT1 is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism. -
细胞定位
ATK1: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. -
数据库链接
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
- Omim: 164730 Human
- Omim: 164731 Human
see all -
别名
- AKT 1 antibody
- AKT 2 antibody
- AKT antibody
see all
图片
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Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/1000 dilution
Lane 1 : Human AKT1 fragment recombinant protein
Lane 2 : Human AKT2 fragment recombinant protein
Lane 3 : Human AKT3 fragment recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human AKT1 fragment recombinant protein contains aa281-480 with a His-Tag®. Human AKT2 fragment recombinant protein contains aa282-481 with a His-Tag®. Human AKT3 fragment recombinant protein contains aa351-479 with a His-Tag®.
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![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264335-WB-2.jpg)
Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/5000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264334-WB-3.jpg)
Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 8 seconds; Lane 2: 2 seconds.
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![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264333-182729WB4.jpg)
Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 2,3 and 4: 5 seconds; Lane 5,6 and 7: 3 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264332-182729IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Human tonsil is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264331-ihc-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Human bladder cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264330-ihc-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Mouse stomach is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264329-ihc-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Rat cerebrum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264327-IF-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AKT1 + AKT2 with ab182729 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264326-IF-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling AKT1 + AKT2 with ab182729 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264325-FC-1.jpg)
Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AKT1 + AKT2 with ab182729 at 1/600 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264324-FC-2.jpg)
Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling AKT1 + AKT2 with ab182729 at 1/600 dilution (red) compared with a RabbitIgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-264323-182729ip1.jpg)
Immunoprecipitation - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)AKT1/2 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182729 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182729 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10µg (Input).
Lane 2: ab182729 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab182729 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.cn/ps/products/182/ab182729/Images/ab182729-14-benefits-of-recombinant-antibodies.png)
Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (19)
ab182729 被引用在 19 文献中.
- Che Y et al. CIP2A interacts with AKT1 to promote the malignant biological behaviors of oral squamous cell carcinoma by upregulating the GSK‑3β/β‑catenin pathway. Exp Ther Med 26:514 (2023). PubMed: 37840566
- Li X et al. Mechanistic Insights of Qingre Jiedu Recipe Based on Network Pharmacology Approach against Heart Failure. Evid Based Complement Alternat Med 2022:9024394 (2022). PubMed: 35140803
- Wang X et al. Calycosin as a Novel PI3K Activator Reduces Inflammation and Fibrosis in Heart Failure Through AKT-IKK/STAT3 Axis. Front Pharmacol 13:828061 (2022). PubMed: 35264961
- Du HY et al. Ligustrazine protects against chronic hypertensive glaucoma in rats by inhibiting autophagy via the PI3K-Akt/mTOR pathway. Mol Vis 27:725-733 (2021). PubMed: 35035207
- Wei H et al. BRD4 Inhibition Protects Against Myocardial Ischemia/Reperfusion Injury by Suppressing Inflammation and Oxidative Stress Through the PI3K/AKT Signaling Pathway. J Cardiovasc Pharmacol 78:839-846 (2021). PubMed: 34524258