Anti-AKR1C3抗体(ab84327)
Key features and details
- Rabbit polyclonal to AKR1C3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Cow, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-AKR1C3抗体
参阅全部 AKR1C3 一抗 -
描述
兔多克隆抗体to AKR1C3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Cow, Human -
免疫原
A 15 amino acid peptide corresponding to the C terminal residues of human AKR1C3 (P42330).
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阳性对照
- Human fetal liver lysates; human lung tissue; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 2% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84327于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
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| IHC-P |
1/100 - 1/500.
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| ICC/IF |
1/50 - 1/200.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
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IHC-P
1/100 - 1/500. |
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ICC/IF
1/50 - 1/200. |
靶标
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功能
Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. -
组织特异性
Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. The dominant HSD in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate. -
序列相似性
Belongs to the aldo/keto reductase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8644 Human
- Omim: 603966 Human
- SwissProt: P42330 Human
- Unigene: 78183 Human
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别名
- 17 beta HSD 5 antibody
- 17 beta hydroxysteroid dehydrogenase type 5 antibody
- 17-beta-HSD 5 antibody
see all
图片
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Western blot - Anti-AKR1C3 antibody (ab84327)Anti-AKR1C3 antibody (ab84327) at 1/500 dilution + human fetal liver lysate
Predicted band size: 36 kDa
Observed band size: 36 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1C3 antibody (ab84327)ab84327, at a 1/100 dilution, staining AKR1C3 in formalin fixed, paraffin embedded human lung tissue by Immunohistochemistry. Note cytoplasmic staining. -
Immunocytochemistry/ Immunofluorescence - Anti-AKR1C3 antibody (ab84327)ab84327, at a 1/50 dilution, staining AKR1C3 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab84327 被引用在 17 文献中.
- Ma S et al. Single-cell sequencing analysis reveals development and differentiation trajectory of Schwann cells manipulated by M. leprae. PLoS Negl Trop Dis 17:e0011477 (2023). PubMed: 37478057
- Sano A et al. Protection of 6-OHDA neurotoxicity by PGF2a through FP-ERK-Nrf2 signaling in SH-SY5Y cells. Toxicology 450:152686 (2021). PubMed: 33486071
- Kowalczyk-Zieba I et al. Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling. Animals (Basel) 11:N/A (2021). PubMed: 34573602
- Xu Z et al. Nuclear receptor ERRa contributes to castration-resistant growth of prostate cancer via its regulation of intratumoral androgen biosynthesis. Theranostics 10:4201-4216 (2020). PubMed: 32226548
- Sorf A et al. Targeting Pharmacokinetic Drug Resistance in Acute Myeloid Leukemia Cells with CDK4/6 Inhibitors. Cancers (Basel) 12:N/A (2020). PubMed: 32560251