Anti-AKR1C1/AKR1C2抗体[4B6AF3] (ab131375)
Key features and details
- Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Related conjugates and formulations
概述
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产品名称
Anti-AKR1C1/AKR1C2抗体[4B6AF3]
参阅全部 AKR1C1/AKR1C2 一抗 -
描述
小鼠单克隆抗体[4B6AF3] to AKR1C1/AKR1C2 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Cow -
免疫原
Full length native protein (purified). This information is considered to be commercially sensitive.
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阳性对照
- WB: HeLa, HepG2, HLH, HHH, MLH, RLH and RHH tissue samples ICC/IF: Hela cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 0.877% Sodium chloride, 0.357% HEPES -
Concentration information loading...
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纯化说明
Purity >90% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
4B6AF3 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131375于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa.
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ICC/IF |
Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells)
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说明 |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells) |
靶标
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数据库链接
- SwissProt: P52895 Human
- SwissProt: Q04828 Human
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别名
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
- 20-alpha-HSD antibody
- 20-alpha-hydroxysteroid dehydrogenase antibody
see all
图片
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All lanes : Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : HeLa Cell Lysate at 15 µg
Lane 3 : HepG2 Cell Lysate at 15 µg
Lane 4 : Human Liver Homogenate (HLH) at 10 µg
Lane 5 : Human Heart Homogenate (HHH) at 10 µg
Lane 6 : Mouse Liver Homogenate (MLH) at 10 µg
Lane 7 : Rat Liver Homogenate (RLH) at 10 µg
Lane 8 : Rat Heart Homogenate (RHH) at 10 µg
Secondary
All lanes : Goat anti-Mouse AP at 1/3000 dilution
Predicted band size: 37 kDa
0.3% PBST was used as a blocking buffer in all incubation steps. The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the Goat anti-Mouse secondary antibody. -
Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml.
The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab131375 被引用在 4 文献中.
- Hornung RS et al. Sigma-1 receptors and progesterone metabolizing enzymes in nociceptive sensory neurons of the female rat trigeminal ganglia: A neural substrate for the antinociceptive actions of progesterone. Mol Pain 18:17448069211069255 (2022). PubMed: 35040378
- Li C et al. High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer. J Biomol Screen 21:101-7 (2016). PubMed: 26318406
- Connarn JN et al. Metabolism of bupropion by carbonyl reductases in liver and intestine. Drug Metab Dispos 43:1019-27 (2015). PubMed: 25904761
- Li C et al. AEG-1 Promotes Metastasis Through Downstream AKR1C2 and NF1 in Liver Cancer. Oncol Res 22:203-211 (2014). PubMed: 26351209