AB179448

重组Anti-AKR1C1/AKR1C2抗体[EPR11542]

Anti-AKR1C1/AKR1C2 antibody [EPR11542]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal AKR1C1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.

查看别名

DDH, DDH1, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-hydroxysteroid dehydrogenase, Chlordecone reductase homolog HAKRC, Dihydrodiol dehydrogenase 1, High-affinity hepatic bile acid-binding protein, 20-alpha-HSD, DD1, HBAB

5 Images

Flow Cytometry (Intracellular) - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Intracellular flow cytometric analysis of permeabilized U87-MG cellslabeling AKR1C1/AKR1C2 with ab179448 at 1/10 dilution (red),compared toa rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Immunocytochemical/immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (human glioblastoma- astrocytoma epithelial cell) cells labelling AKR1C1/AKR1C2 with primary antibody anti-AKR1C1/AKR1C2 (ab179448) at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in U-87 MG cell line. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to counterstain tubulin at 1 : 200 dilution. The nuclear counter stain is DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

AKR1C1/AKR1C2 was immunoprecipitated from U-87 MG lysates with ab179448 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab179448 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10 μg Lane 2 : ab179448 in U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab179448 in U-87 MG whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/30 dilution

All lanes:

U-87 MG lysates

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Supplier Data

Western blot - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/1000 dilution

Lane 1:

His-tagged human AKR1C1 recombinant protein, full-length

Lane 2:

His-tagged human AKR1C2 recombinant protein, full-length

Lane 3:

His-tagged human AKR1C3 recombinant protein, full-length

Lane 4:

His-tagged human AKR1C4 recombinant protein, full-length

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa

false

Exposure time: 40s

Supplier Data

Western blot - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

All lanes:

Western blot - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (ab179448) at 1/1000 dilution

Lane 1:

HepG2 lysate at 10 µg

Lane 2:

U87-MG lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Predicted band size: 37 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11542

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, Flow Cyt (Intra), ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

AKR1C1 and AKR1C2 (Swiss Prot: Q04828/P52895) sequences are 98% identical

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The AKR1C1 and AKR1C2 are members of the aldo-keto reductase family. These enzymes also known as dihydrodiol dehydrogenases reduce a variety of substrates including steroids prostaglandins and xenobiotics. AKR1C1 has a molecular mass of approximately 37 kDa while AKR1C2 has a similar but slightly distinct mass. These proteins are expressed in various tissues including the liver prostate and mammary glands where they perform their enzymatic functions.

Biological function summary

The AKR1C1/AKR1C2 enzymes play significant roles in steroid hormone metabolism. They modulate the conversion of active hormones to their inactive forms and vice versa affecting the cellular concentration of these hormones. The proteins do not function as part of a larger complex but influence multiple biochemical processes through their enzymatic actions. Their roles in the regulation of sex steroids are important for maintaining normal cellular activity and tissue homeostasis.

Pathways

AKR1C1 and AKR1C2 are involved in intricate steroid hormone signaling and metabolism pathways. They engage with the androgen and estrogen receptor signaling pathways by modulating hormone availability. The interplay with enzymes like 5-alpha reductase and aromatase highlights their role in the balance of active and inactive hormone forms within these pathways. Their function can influence cellular proliferation and differentiation processes within different tissues.

AKR1C1/AKR1C2 have links to hormone-related cancers such as prostate and breast cancer. These enzymes impact the local hormone levels affecting cancer progression and response to therapy. Alterations in AKR1C1 and AKR1C2 expression or function can lead to hormone imbalances influencing cancer risk and treatment outcomes. Through their modulation of steroid hormones they connect to the activity of proteins like androgen and estrogen receptors which are critical in the pathogenesis of hormone-driven cancers.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids (PubMed : 19218247). Most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentrations of NADPH (PubMed : 14672942). Displays a broad positional specificity acting on positions 3, 17 and 20 of steroids and regulates the metabolism of hormones like estrogens and androgens (PubMed : 10998348). May also reduce conjugated steroids such as 5alpha-dihydrotestosterone sulfate (PubMed : 19218247). Displays affinity for bile acids (PubMed : 8486699).

See full target information AKR1C1

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of the Endocrine Society 9:bvaf087 PubMed40421431

2025

Radioactive Tracing of Testosterone Reveals Minimal Formation of 5α-DHT in SGBS Cells and Human Primary Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Andress Huacachino,Cátia F Marques,Clementina Mesaros,Trevor M Penning

The Journal of pathology 264:68-79 PubMed39022843

2024

Histone demethylase PHF8 promotes prostate cancer metastasis via the E2F1-SNAI1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ze Wang,Peng Tang,Haiyang Xiao,Song Peng,Jian Chen,Yapeng Wang,Jing Xu,Qian Yan,Junying Zhang,Jie Deng,Qiang Ma,Hailin Zhu,Weiming Luo,Dianzheng Zhang,Luofu Wang,Jun Qin,Weihua Lan,Jun Jiang,Qiuli Liu

Communications chemistry 7:95 PubMed38684887

2024

Discovery of an Aldo-Keto reductase 1C3 (AKR1C3) degrader.

Applications

Unspecified application

Species

Unspecified reactive species

Angelica V Carmona,Shirisha Jonnalagadda,Alfie M Case,Krishnaiah Maddeboina,Sravan K Jonnalagadda,Louise F Dow,Ling Duan,Trevor M Penning,Paul C Trippier

Cell death & disease 13:1067 PubMed36550099

2022

ABX-1431 inhibits the development of endometrial adenocarcinoma and reverses progesterone resistance by targeting MGLL.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Ma,Min Xia,Lina Wei,Kui Guo,Rui Sun,Yao Liu,Chunping Qiu,Jie Jiang

Medical oncology (Northwood, London, England) 39:202 PubMed36175596

2022

CD36 accelerates the progression of hepatocellular carcinoma by promoting FAs absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Lide Tao,Xiangmin Ding,Lele Yan,Guangcai Xu,Peijian Zhang,Anlai Ji,Lihong Zhang

International journal of molecular sciences 21: PubMed32102467

2020

Analysis and Identification of Tumorigenic Targets of MicroRNA in Cancer Cells by Photoreactive Chemical Probes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyu Su,Tsogzolmaa Ganbold,Huricha Baigude

Chemical research in toxicology 32:2538-2551 PubMed31746589

2019

Induction of the Antioxidant Response by the Transcription Factor NRF2 Increases Bioactivation of the Mutagenic Air Pollutant 3-Nitrobenzanthrone in Human Lung Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica R Murray,Laureano de la Vega,John D Hayes,Ling Duan,Trevor M Penning

View all publications

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-AKR1C1/AKR1C2抗体[EPR11542]

Anti-AKR1C1/AKR1C2 antibody [EPR11542]

Flow Cytometry (Intracellular) - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Immunoprecipitation - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Western blot - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

Western blot - Anti-AKR1C1/AKR1C2 antibody [EPR11542] (AB179448)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性