Anti-AKAP9抗体[17G10] (ab32679)
Key features and details
- Mouse monoclonal [17G10] to AKAP9
- Suitable for: IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-AKAP9抗体[17G10]
参阅全部 AKAP9 一抗 -
描述
小鼠单克隆抗体[17G10] to AKAP9 -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Dog, Chimpanzee -
免疫原
Synthetic peptide:
QFRQRKAQSDGQSPS
, corresponding to amino acids 31-45 of Human AKAP9 -
阳性对照
- ICC: A431, HeLa and NIH-3T3 cells. IHC-P: Human pancreas tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
17G10 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32679于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/10 - 1/100.
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ICC |
Use a concentration of 4 µg/ml.
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说明 |
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IHC-P
1/10 - 1/100. |
ICC
Use a concentration of 4 µg/ml. |
靶标
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功能
Binds to type II regulatory subunits of protein kinase A. Scaffolding protein that assembles several protein kinases and phosphatases on the centrosome and Golgi apparatus. May be required to maintain the integrity of the Golgi apparatus. Isoform 4 is associated with the N-methyl-D-aspartate receptor and is specifically found in the neuromuscular junction (NMJ) as well as in neuronal synapses, suggesting a role in the organization of postsynaptic specializations. -
组织特异性
Widely expressed. Isoform 4 is highly expressed in skeletal muscle and in pancreas. -
疾病相关
Defects in AKAP9 are the cause of long QT syndrome type 11 (LQT11) [MIM:611820]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy. -
结构域
RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Golgi apparatus. Cytoplasmic in parietal cells. - Information by UniProt
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数据库链接
- Entrez Gene: 10142 Human
- Entrez Gene: 100986 Mouse
- Omim: 604001 Human
- SwissProt: Q99996 Human
- SwissProt: Q70FJ1 Mouse
- Unigene: 651221 Human
- Unigene: 46044 Mouse
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别名
- A kinase (PRKA) anchor protein (yotiao) 9 antibody
- A kinase (PRKA) anchor protein 9 antibody
- A kinase anchor protein 350kDa antibody
see all
图片
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Immunocytochemical analysis of AKAP9 in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:20 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemical analysis of AKAP9 in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunocytochemical analysis of AKAP9 in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:100 overnight at 4 C and incubated with a DyLight-488 conjugated secondary antibody. AKAP9 staining (green) F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP9 antibody [17G10] (ab32679)
Immunohistochemistry was performed on normal biopsies of deparaffinized Human pancreas tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a AKAP9 monoclonal antibody (ab32679) at a dilution of 1:20 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab32679 被引用在 2 文献中.
- Wu S et al. AKAP9 Upregulation Predicts Unfavorable Prognosis in Pediatric Acute Myeloid Leukemia and Promotes Stemness Properties via the Wnt/β-Catenin Pathway. Cancer Manag Res 14:157-167 (2022). PubMed: 35046723
- Lehka L et al. Loss of Unconventional Myosin VI Affects cAMP/PKA Signaling in Hindlimb Skeletal Muscle in an Age-Dependent Manner. Front Physiol 13:933963 (2022). PubMed: 35837016