重组Anti-AKAP14抗体[EPR12955] - C-terminal (ab180160)
![Western blot - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)](https://www.abcam.cn/ps/products/180/ab180160/Images/ab180160-208767-ab180160WB.jpg "Western blot - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12955] to AKAP14 - C-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-AKAP14抗体[EPR12955] - C-terminal
参阅全部 AKAP14 一抗 -
描述
兔单克隆抗体[EPR12955] to AKAP14 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IP, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, HUVEC, NCI-H460, A549 and Human fetal kidney lysates; A549 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12955 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180160于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
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| IP |
1/10 - 1/100.
|
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| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
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IP
1/10 - 1/100. |
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ICC/IF
1/100 - 1/250. |
靶标
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相关性
Binds to type II regulatory subunits of protein kinase A and anchors/targets them -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 158798 Human
- Entrez Gene: 434756 Mouse
- Entrez Gene: 60332 Rat
- Omim: 300462 Human
- SwissProt: Q86UN6 Human
- SwissProt: O35817 Rat
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别名
- A kinase (PRKA) anchor protein 14 antibody
- A kinase anchor protein 14 antibody
- A kinase anchor protein 28 kDa antibody
see all
图片
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Western blot - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)All lanes : Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : NCI-H460 cell lysate
Lane 4 : A549 cell lysate
Lane 5 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)](https://www.abcam.cn/ps/products/180/ab180160/Images/ab180160-208766-ab180160IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)Immunofluorescence analysis of A549 cells labeling AKAP14 with ab180160 at a 1/100 dilution.
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![Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)](https://www.abcam.cn/ps/products/180/ab180160/Images/ab180160-208764-ab180160IP.jpg)
Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing AKAP14, using ab180160 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)](https://www.abcam.cn/ps/products/180/ab180160/Images/ab180160-4-benefits-of-recombinant-antibodies.png)
Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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