重组Anti-AKAP associated Sperm蛋白/ROPN1L抗体[EPR11266(B)] (ab173298)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-196725-anti-akap-associated-sperm-protein-antibody-epr11266b-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11266(B)] to AKAP associated Sperm Protein/ROPN1L
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-AKAP associated Sperm蛋白/ROPN1L抗体[EPR11266(B)]
参阅全部 AKAP associated Sperm Protein/ROPN1L 一抗 -
描述
兔单克隆抗体[EPR11266(B)] to AKAP associated Sperm蛋白/ROPN1L -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-P, WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human testis lysate, Human testis tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11266(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173298于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/10 - 1/100.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
|
| 说明 |
|---|
|
IP
1/10 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). |
靶标
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序列相似性
Belongs to the ropporin family.
Contains 1 RIIa domain. - Information by UniProt
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数据库链接
- Entrez Gene: 83853 Human
- Omim: 611756 Human
- SwissProt: Q96C74 Human
- Unigene: 381089 Human
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别名
- ropporin 1 like antibody
- AKAP-associated sperm protein antibody
- ASP antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling AKAP associated Sperm Protein/ROPN1L with ab173298 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-221510-anti-akap-associated-sperm-protein-antibody-epr11266b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)ab173298 showing +ve staining in Human normal testis tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-201857-anti-akap-associated-sperm-protein-antibody-epr11266b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)ab173298 showing -ve staining in Human breast carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-201856-anti-akap-associated-sperm-protein-antibody-epr11266b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)ab173298 showing -ve staining in Human cervical carcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-201855-anti-akap-associated-sperm-protein-antibody-epr11266b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)ab173298 showing -ve staining in Human skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-201854-anti-akap-associated-sperm-protein-antibody-epr11266b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)ab173298 showing -ve staining in Human normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-196727-anti-akap-associated-sperm-protein-antibody-epr11266b-western-blot.jpg)
Western blot - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298) at 1/1000 dilution + Human testis lysate at 10 µg
Predicted band size: 26 kDa -
![Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-196726-anti-akap-associated-sperm-protein-antibody-epr11266b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)Immunoprecipitation. ab173298 at 1/1000 staining AKAP associated Sperm Protein/ROPN1L immunoprecipitated from Human tetis lysate using ab173297 at 1/10.
Lane 2: 1X PBS (negative control) .
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![Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)](https://www.abcam.cn/ps/products/173/ab173298/Images/ab173298-10-benefits-of-recombinant-antibodies.png)
Anti-AKAP associated Sperm Protein/ROPN1L antibody [EPR11266(B)] (ab173298)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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