Anti-AK5抗体[OTI1D6] (ab117927)
![Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.cn/ps/products/117/ab117927/Images/AK5-Primary-antibodies-ab117927-1.jpg "Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)")
Key features and details
- Mouse monoclonal [OTI1D6] to AK5
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human, African green monkey
- Isotype: IgG1
概述
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产品名称
Anti-AK5抗体[OTI1D6]
参阅全部 AK5 一抗 -
描述
小鼠单克隆抗体[OTI1D6] to AK5 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human AK5 aa 1-600. NP_777283. Produced in HEK-293T cells.
Database link: Q9Y6K8 -
阳性对照
- WB: HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA. ICC/IF: COS-7 transiently transfected by pCMV6-ENTRY AK5 cDNA. Flow Cyt: HEK-293T cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1.
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常规说明
Clone OTI1D6 (formerly 1D6).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI1D6 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117927于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 63 kDa.
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| ICC/IF |
1/100.
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| Flow Cyt |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/2000. Predicted molecular weight: 63 kDa. |
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ICC/IF
1/100. |
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Flow Cyt
Use at an assay dependent concentration. |
靶标
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功能
Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. -
组织特异性
Brain specific. -
序列相似性
Belongs to the adenylate kinase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 26289 Human
- Omim: 608009 Human
- SwissProt: Q9Y6K8 Human
- Unigene: 559718 Human
- Unigene: 597002 Human
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别名
- Adenylate kinase 5 antibody
- Adenylate kinase 6 antibody
- Adenylate kinase isoenzyme 5 antibody
see all
图片
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Western blot - Anti-AK5 antibody [OTI1D6] (ab117927)All lanes : Anti-AK5 antibody [OTI1D6] (ab117927) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY AK5 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.cn/ps/products/117/ab117927/Images/AK5-Primary-antibodies-ab117927-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AK5 antibody [OTI1D6] (ab117927)pCMV6-ENTRY AK5 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for AK5 using ab117927 at a 1/100 dilution in ICC/IF.
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![Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)](https://www.abcam.cn/ps/products/117/ab117927/Images/ab117927-314116-117927777.jpg)
Flow Cytometry - Anti-AK5 antibody [OTI1D6] (ab117927)Flow Cytometry analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with AK5 (Myc-DDK-tagged)-Human adenylate kinase 5 (AK5), transcript variant 1, using ab117927 (red) compared to an empty vector control plasmid (blue).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab117927 尚未被引用在任何文献中。