AB128960

重组Anti-AIPL1抗体[EPR7711]

Anti-AIPL1 antibody [EPR7711]

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal AIPL1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

AIPL2, AIPL1, Aryl-hydrocarbon-interacting protein-like 1

3 Images

Flow Cytometry (Intracellular) - Anti-AIPL1 antibody [EPR7711] (AB128960)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-AIPL1 antibody [EPR7711] (AB128960)

ab128960, at 1/10 dilution staining AIPL1 (red) in permeabilized Y79 cells, compared with a negative rabbit IgG (green) by intracellular flow cytometry.

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Western blot - Anti-AIPL1 antibody [EPR7711] (AB128960)

All lanes:

Western blot - Anti-AIPL1 antibody [EPR7711] (ab128960) at 1/10000 dilution

All lanes:

Y79 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

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OI-RD Scanning - Anti-AIPL1 antibody [EPR7711] (AB128960)

OI-RD Scanning

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OI-RD Scanning - Anti-AIPL1 antibody [EPR7711] (AB128960)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-AIPL1 antibody [EPR7711] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7711

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AIPL1 also known as aryl hydrocarbon receptor-interacting protein-like 1 is a protein that plays an important mechanical role in the cellular environment. It has a molecular mass of approximately 42 kDa. AIPL1 is expressed mainly in the retina further appearing in photoreceptor cells where its function becomes important for proper cell operation. This protein interacts with other molecular structures directly affecting processes essential to cellular function.

Biological function summary

AIPL1 assists in maintaining the stability and proper folding of photoreceptor PDE6 a cyclic GMP phosphodiesterase an important component of the phototransduction complex. It acts as a chaperone ensuring that PDE6 which is critical for visual processes stays correctly assembled and functional. Its role in this complex highlights its involvement in the health of photoreceptor cells and indirectly supports the conversion of light into neural signals within the eye.

Pathways

AIPL1 participates significantly within the phototransduction pathway. This is a vital pathway required for vision where it directly influences the regulation of PDE6 activity. AIPL1's association with PDE6 links it to further elements like the guanylate cyclase pathway important for replenishing cGMP levels post-phototransduction. Interactions with proteins such as chaperonin-containing TCP-1 (CCT) highlight its role in the folding and proper functioning of phototransduction components.

Mutations in AIPL1 are associated with Leber congenital amaurosis (LCA) a severe inherited retinal dystrophy that leads to blindness or severe vision impairment from a young age. The loss of function or malfunction of AIPL1 affects PDE6 leading to the buildup of misfolded proteins which impairs photoreceptor cell survival and function. Additionally study of similar AIPL1 mutations sheds light on a subset of retinitis pigmentosa linking abnormalities within this protein to progressive vision disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May be important in protein trafficking and/or protein folding and stabilization.

See full target information AIPL1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 29:145 PubMed37884883

2023

Leveraging a disulfidptosis-related signature to predict the prognosis and immunotherapy effectiveness of cutaneous melanoma based on machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhao,Yanjun Wei,Lingjia Fan,Yuanliu Nie,Jianan Li,Renya Zeng,Jixian Li,Xiang Zhan,Lingli Lei,Zhichao Kang,Jiaxin Li,Wentao Zhang,Zhe Yang

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-AIPL1抗体[EPR7711]