Anti-AIMP3/p18抗体(ab31543)
Key features and details
- Rabbit polyclonal to AIMP3/p18
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AIMP3/p18抗体
参阅全部 AIMP3/p18 一抗 -
描述
兔多克隆抗体to AIMP3/p18 -
宿主
Rabbit -
特异性
ab31543 reacts specifically with 21kDa p18 and immunoprecipitates with ATM/ATR -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human AIMP3/p18.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/8000. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/8000. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Positive modulator of ATM response to DNA damage. -
组织特异性
Down-regulated in various cancer tissues. -
序列相似性
Contains 1 GST C-terminal domain. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic under growth arrest conditions. Translocated into the nucleus when growth resumes (S phase) and following DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 9521 Human
- Omim: 609206 Human
- SwissProt: O43324 Human
- Unigene: 602353 Human
- Unigene: 726163 Human
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别名
- AIMP 3 antibody
- AIMP3 antibody
- Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (ab31543)
Immunofluorescence analysis of Hela cells using anti-AIMP3 (p18) antibody (ab31543).
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Western blot - Anti-AIMP3/p18 antibody (ab31543)Anti-AIMP3/p18 antibody (ab31543) at 1/20000 dilution + HEK293 cell line
Predicted band size: 18 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-AIMP3/p18 antibody (ab31543)Immunofluorescence analysis of U2OS cells using anti-AIMP3 (p18) antibody (ab31543).
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Western blot - Anti-AIMP3/p18 antibody (ab31543)Anti-AIMP3/p18 antibody (ab31543) at 1/8000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Secondary
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab31543 被引用在 6 文献中.
- Jin D et al. HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit. Viruses 15:N/A (2023). PubMed: 36851687
- Wang K et al. Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage. Ann Transl Med 8:1670 (2020). PubMed: 33490182
- Gulati P et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB ; Mouse . PubMed: 23359686
- Du Y et al. Elevation of Highly Up-regulated in Liver Cancer (HULC) by Hepatitis B Virus X Protein Promotes Hepatoma Cell Proliferation via Down-regulating p18. J Biol Chem 287:26302-11 (2012). WB ; Human . PubMed: 22685290
- David A et al. RNA Binding Targets Aminoacyl-tRNA Synthetases to Translating Ribosomes. J Biol Chem 286:20688-700 (2011). Human . PubMed: 21460219
- Oh YS et al. Downregulation of lamin A by tumor suppressor AIMP3/p18 leads to a progeroid phenotype in mice. Aging Cell 9:810-22 (2010). WB ; Mouse . PubMed: 20726853