Anti-AIF抗体- Mitochondrial Marker (ab1998)
Key features and details
- Rabbit polyclonal to AIF - Mitochondrial Marker
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-AIF抗体- Mitochondrial Marker
参阅全部 AIF 一抗 -
描述
兔多克隆抗体to AIF - Mitochondrial Marker -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human AIF aa 500-600.
Database link: O95831-1 -
阳性对照
- WB: Human Caco-2, Daudi, HeLa, HepG2, MCF7, NIH3T3, YB2/0 and K562 cell lysate. Rat and mouse heart lysate. IHC-P: Human retina. ICC/IF: Human U2OS and HeLa cells.
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常规说明
Apoptosis Inducing FactorThe Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
AIF Antibody is affinity chromatography purified via peptide column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). Can be blocked with AIF (internal) peptide (human).
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa). Can be blocked with AIF (internal) peptide (human). |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. -
疾病相关
Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting. -
序列相似性
Belongs to the FAD-dependent oxidoreductase family. -
翻译后修饰
Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. -
细胞定位
Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 51060 Human
- Entrez Gene: 9131 Human
- Entrez Gene: 26926 Mouse
- Entrez Gene: 83533 Rat
- Omim: 300169 Human
- SwissProt: O95831 Human
- SwissProt: Q9Z0X1 Mouse
- SwissProt: Q9JM53 Rat
see all -
别名
- AIFM1 antibody
- AIFM1_HUMAN antibody
- Apoptosis inducing factor 1, mitochondrial antibody
see all
图片
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Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998)All lanes : Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : Rat heart tissue lysate
Lane 3 : Mouse heart tissue lysate
Predicted band size: 67 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody - Mitochondrial Marker (ab1998)Immunohistochemistry of AIF in human retina with anti-AIF (IN) at 10 µg/ml. -
Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998)All lanes : Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/ml
Lane 1 : Caco-2 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 67 kDa -
Western blot - Anti-AIF antibody - Mitochondrial Marker (ab1998)All lanes : Anti-AIF antibody - Mitochondrial Marker (ab1998) at 1 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 67 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody - Mitochondrial Marker (ab1998)Image from Varecha M et al, J Biomed Sci. 2009 Jul 6;16:59, fig 2.ab1998 staining AIF in human U2OS cells by Immunocytochemistry. Samples were fixed using 4% paraformaldehyde.Left image shows fixed cells labeled with ab1998 (red) and cyclophilin A (green).
Right image shows U2OS cell 6 hours after induction of apoptosis by 200 nM staurosporine.Note translocated of AIF to the nucleus upon induction of apoptosis. -
Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody - Mitochondrial Marker (ab1998)ICC image of ab1998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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Datasheet download
文献 (45)
ab1998 被引用在 45 文献中.
- Yu X et al. Apoptin causes apoptosis in HepG-2 cells via Ca2+ imbalance and activation of the mitochondrial apoptotic pathway. Cancer Med 12:8306-8318 (2023). PubMed: 36515089
- Kim H et al. Lethal effects of mitochondria via microfluidics. Bioeng Transl Med 8:e10461 (2023). PubMed: 37206227
- Qi H et al. Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress. Mol Med Rep 28:N/A (2023). PubMed: 37232340
- Abrego J et al. A Cancer Cell-Intrinsic GOT2-PPARδ Axis Suppresses Antitumor Immunity. Cancer Discov 12:2414-2433 (2022). PubMed: 35894778
- Aboouf MA et al. Pro-Apoptotic and Anti-Invasive Properties Underscore the Tumor-Suppressing Impact of Myoglobin on a Subset of Human Breast Cancer Cells. Int J Mol Sci 23:N/A (2022). PubMed: 36232784
