重组Anti-AIDA抗体[EPR20903-53] - BSA and Azide free (ab274421)
![Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)](https://www.abcam.cn/ps/products/274/ab274421/Images/ab274421-410995-anti-aida-antibody-epr2090353-western-blot-embryonic.jpg "Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20903-53] to AIDA - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-AIDA抗体[EPR20903-53] - BSA and Azide free
参阅全部 AIDA 一抗 -
描述
兔单克隆抗体[EPR20903-53] to AIDA - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: MEF, NIH/3T3, RAE 264.7, C2C12, HeLa, U-87 MG, C6 and PC-12 whole cell lysates.
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常规说明
ab274421 is the carrier-free version of ab234419.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20903-53 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274421于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa). |
靶标
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功能
Acts as a ventralizing factor during embryogenesis. Inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling. -
组织特异性
Widely expressed in adult tissues, with highest expression in the heart and skeletal muscle. -
序列相似性
Belongs to the AIDA family. - Information by UniProt
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数据库链接
- Entrez Gene: 64853 Human
- Entrez Gene: 108909 Mouse
- Omim: 612375 Human
- SwissProt: Q96BJ3 Human
- SwissProt: Q8C4Q6 Mouse
- Unigene: 156625 Human
- Unigene: 534965 Human
- Unigene: 290502 Mouse
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别名
- 2610208M17Rik antibody
- AIDA antibody
- AIDA_HUMAN antibody
see all
图片
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Western blot - Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)All lanes : Anti-AIDA antibody [EPR20903-53] (ab234419) at 1/1000 dilution
Lane 1 : Wild-type MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 2 : AIDA knockout MEF whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 4 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 5 : C2C12 (mouse myoblasts myoblast) whole cell lysate
Lane 6 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 7 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 8 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 9 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?This data was developed using ab234419, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab234419 was shown to specifically react with AIDA in wild-type MEF cells as signal was lost in AIDA knockout cells. Wild-type and AIDA knockout samples were subjected to SDS-PAGE. ab234419 and ab52901 (Rabbit anti-beta Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50, 000 dilution and 1/100,000 dilution for 1 hour at room temperature before imaging respectively. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
AIDA knockout MEF cell lysate was kindly provided by our collaborator.
Exposure time: 3 minutes.
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![Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)](https://www.abcam.cn/ps/products/274/ab274421/Images/ab274421-2-benefits-of-recombinant-antibodies.png)
Anti-AIDA antibody [EPR20903-53] - BSA and Azide free (ab274421)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab274421 尚未被引用在任何文献中。
