AB180861

重组Anti-Ahsp抗体[EPR12319]

Name: Ahsp抗体[EPR12319] (ab180861)| Abcam中文官网
Brand: Abcam Limited
SKU: AB180861
Price: 2737 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Ahsp antibody [EPR12319]

RabMAb
Recombinant
了解详情

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal AHSP antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication.

查看别名

EDRF, ERAF, AHSP, Alpha-hemoglobin-stabilizing protein, Erythroid differentiation-related factor, Erythroid-associated factor

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (AB180861)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (AB180861)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Ahsp with ab180861 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (AB180861)

Immunohistochemical analysis of paraffin-embedded Human fetal spleen tissue labeling Ahsp with ab180861 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Ahsp antibody [EPR12319] (AB180861)

Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab180861 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-Ahsp antibody [EPR12319] (ab180861)

Predicted band size: 12 kDa

false

Supplier Data

Western blot - Anti-Ahsp antibody [EPR12319] (AB180861)

All lanes:

Western blot - Anti-Ahsp antibody [EPR12319] (ab180861) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Predicted band size: 12 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12319

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

style

left-column

产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The AHSP protein also known as Alpha Hemoglobin Stabilizing Protein plays an important role in the stabilization of alpha-globin chains. This protein has a mass of approximately 11 kDa and is expressed in erythroid cells which are involved in red blood cell development and function. By binding to alpha-globin the AHSP protein assists in preventing harmful aggregation and degradation of these chains which is important in hemoglobin assembly and stability.

Biological function summary

AHSP protein assists in preventing the precipitation of excess free alpha-globin chains within erythroid cells. It acts as a chaperone guiding the proper folding of alpha-globin and facilitating the formation of hemoglobin. This protein does not work alone; it operates as a component of the erythrocytic protein maturation complex working closely with beta-globin during erythropoiesis to ensure effective hemoglobin production.

Pathways

The AHSP protein integrates into cellular processes by playing a role in the hemoglobin synthesis pathway. It is a part of the broader erythroid differentiation pathway and ensures effective erythrocyte maturation and function. AHSP protein is related to HBA1 and HBA2 proteins through these pathways as they are all important components during hemoglobin formation.

Malfunction or reduced expression of the AHSP protein links to various hemoglobinopathies such as beta-thalassemia and alpha-thalassemia. In these conditions imbalances in the globin chains cause abnormal erythrocyte formation and impaired oxygen transport. The AHSP protein connects to HBB and HBA1 proteins when considering these disorders as its stabilizing role influences the equilibrium between alpha- and beta-globin chains important for effective erythrocyte functionality.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia.

See full target information AHSP

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 18:3251-3262 PubMed31640472

2019

hypermethylation promotes heart failure by inhibiting CXCL14 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Longhuan Zeng,Nanyuan Gu,Jiayi Chen,Guangyong Jin,Yongke Zheng

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Ahsp抗体[EPR12319]

Anti-Ahsp antibody [EPR12319]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (AB180861)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ahsp antibody [EPR12319] (AB180861)

Immunoprecipitation - Anti-Ahsp antibody [EPR12319] (AB180861)

Western blot - Anti-Ahsp antibody [EPR12319] (AB180861)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原