AB249942

重组Anti-AHAS抗体[EPR12843] - BSA and Azide free

Anti-AHAS antibody [EPR12843] - BSA and Azide free

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal AHAS antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

AHAS, HACL2, ILVBL, 2-hydroxyacyl-CoA lyase 2, Acetolactate synthase-like protein, IlvB-like protein

2 Images

Flow Cytometry (Intracellular) - Anti-AHAS antibody [EPR12843] - BSA and Azide free (AB249942)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AHAS antibody [EPR12843] - BSA and Azide free (AB249942)

This data was developed using ab177157, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Hela cells labeling AHAS with ab177157 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

Supplier Data

Western blot - Anti-AHAS antibody [EPR12843] - BSA and Azide free (AB249942)

This data was developed using ab177157, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AHAS antibody [EPR12843] (<a href='/products/primary-antibodies/ahas-antibody-epr12843-ab177157'>ab177157</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 67 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-AHAS antibody [EPR12843]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12843

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

ab249942 is the carrier-free version of ab177157.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The acetohydroxyacid synthase (AHAS) also known as acetolactate synthase (ALS) is an enzyme that plays an important role in the biosynthesis of branched-chain amino acids. With a molecular mass of approximately 170 kDa AHAS catalyzes the first common step in the biosynthetic pathways of valine leucine and isoleucine. This enzyme is widely expressed in plants bacteria and fungi. AHAS is a holoenzyme that consists of a large catalytic subunit and a smaller regulatory subunit showing its complex structure and diverse regulatory control.

Biological function summary

AHAS facilitates the condensation of two pyruvate molecules to form acetolactate or a pyruvate and ketoisovalerate to form acetohydroxybutyrate. This condensation reaction is a critical step in the synthesis of all three branched-chain amino acids which are essential components for protein construction in various organisms. As a part of a multi-enzyme complex the activity of AHAS is also modulated by feedback mechanisms from the end products of its pathway ensuring the balance in amino acid production and cellular homeostasis.

Pathways

AHAS integrates into the branched-chain amino acid biosynthesis pathway directly taking its place at the start. This pathway connects to amino acid metabolism which is essential for protein synthesis. AHAS is intertwined with proteins like dihydroxyacid dehydratase and branched-chain aminotransferase which continue the pathway downstream. These interactions highlight AHAS's significance in creating amino acids necessary for organismal growth and development.

AHAS relates prominently to conditions involving imbalances in amino acid metabolism. Alterations in AHAS function can lead to metabolic disorders such as maple syrup urine disease although it primarily involves branched-chain alpha-keto acid dehydrogenase complex. In some cases herbicides target AHAS poisoning the mechanism in plants and disrupting their growth. Understanding the function of AHAS helps us to comprehend the architecture of metabolic diseases and assists in designing therapeutic approaches even though the direct connection with human metabolic disorders remains mainly indirect.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Endoplasmic reticulum 2-OH acyl-CoA lyase involved in the cleavage (C1 removal) reaction in the fatty acid alpha-oxydation in a thiamine pyrophosphate (TPP)-dependent manner. Involved in the phytosphingosine degradation pathway.

See full target information ILVBL

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-AHAS抗体[EPR12843] - BSA and Azide free