Anti-AGXT抗体[1F9BC7] (ab110313)
![Immunocytochemistry/ Immunofluorescence - Anti-AGXT antibody [1F9BC7] (ab110313)](https://www.abcam.cn/ps/products/110/ab110313/Images/ab110313-123593-anti-agxt-antibody-1f9bc7-immunofluorescence.gif "Immunocytochemistry/ Immunofluorescence - Anti-AGXT antibody [1F9BC7] (ab110313)")
Key features and details
- Mouse monoclonal [1F9BC7] to AGXT
- Suitable for: ICC/IF, IP
- Reacts with: Cow, Human
- Isotype: IgG1
概述
-
产品名称
Anti-AGXT抗体[1F9BC7]
参阅全部 AGXT 一抗 -
描述
小鼠单克隆抗体[1F9BC7] to AGXT -
宿主
Mouse -
经测试应用
适用于: ICC/IF, IPmore details -
种属反应性
与反应: Cow, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- HepG2 cells; Human and Rat liver, Bovine heart and HepG2 cell lysates.
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯化说明
ab110313 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
1F9BC7 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110313于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IP
Use at an assay dependent concentration. |
靶标
-
组织特异性
Liver. -
疾病相关
Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1) [MIM:259900]; also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. -
序列相似性
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. -
细胞定位
Peroxisome. Mitochondrion matrix. Except in some HP1 patients where AGT is found in the mitochondrial matrix. - Information by UniProt
-
数据库链接
- Entrez Gene: 189 Human
- Omim: 604285 Human
- SwissProt: P21549 Human
- Unigene: 144567 Human
-
别名
- AGT antibody
- AGT1 antibody
- Agxt antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-AGXT antibody [1F9BC7] (ab110313)Immunocytochemistry analysis using ab110313 at 5 ug/ml staining AGXT in HepG2 cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green).
-
![Immunoprecipitation - Anti-AGXT antibody [1F9BC7] (ab110313)](https://www.abcam.cn/ps/products/110/ab110313/Images/ab110313-123592-anti-agxt-antibody-1f9bc7-immunoprecipitation.gif)
Immunoprecipitation - Anti-AGXT antibody [1F9BC7] (ab110313)Using MitoSciences' standard immunoprecipitation protocol, ab110313 precipicates with the 43kDa AGXT protein in human liver samples. Very small amounts of AGXT are immunoprecipitated from bovine heart, rat liver or human HepG2 cultured cell lysate. Identity of this protein was confirmed by mass spectrometry. This gel was stained with silver.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab110313 尚未被引用在任何文献中。