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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi

Anti-AGO3抗体(ab3593)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (1)

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Western blot - Anti-AGO3 antibody (ab3593)
  • Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)

Key features and details

  • Rabbit polyclonal to AGO3
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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概述

  • 产品名称

    Anti-AGO3抗体
    参阅全部 AGO3 一抗
  • 描述

    兔多克隆抗体to AGO3
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Argonaute and Piwi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human AGO3 protein (ab131912)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3593于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ICC/IF (1)
1/200.
说明
WB
1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ICC/IF
1/200.

靶标

  • 功能

    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
  • 序列相似性

    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 细胞定位

    Cytoplasm > P-body.
  • Target information above from: UniProt accession Q9H9G7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 192669 Human
    • Entrez Gene: 214150 Mouse
    • Entrez Gene: 313593 Rat
    • GenBank: NP_079128 Human
    • GenBank: NP_803171 Human
    • GenBank: NP_700451 Mouse
    • Omim: 607355 Human
    • SwissProt: Q9H9G7 Human
    • SwissProt: Q8CJF9 Mouse
    • Unigene: 657659 Human
    • Unigene: 440915 Mouse
    • Unigene: 482388 Mouse
    • Unigene: 483093 Mouse
    see all
  • 别名

    • 5730550L01Rik antibody
    • Ago 3 antibody
    • Ago3 antibody
    • AGO3_HUMAN antibody
    • argonaute 3 antibody
    • Argonaute3 antibody
    • eIF 2C 3 antibody
    • eIF-2C 3 antibody
    • eIF2C 3 antibody
    • Eif2c3 antibody
    • EIF2C3 protein antibody
    • Eukaryotic translation initiation factor 2C 3 antibody
    • Eukaryotic translation initiation factor 2C3 antibody
    • FLJ12765 antibody
    • hAgo3 antibody
    • MGC86946 antibody
    • Protein argonaute-3 antibody
    see all

图片

  • Western blot - Anti-AGO3 antibody (ab3593)
    Western blot - Anti-AGO3 antibody (ab3593)
    All lanes : Anti-AGO3 antibody (ab3593) at 2 µg/ml

    Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)
    Immunocytochemistry/ Immunofluorescence - Anti-AGO3 antibody (ab3593)

    Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al  (2006).

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (1)

发表研究结果有使用 ab3593?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3593 被引用在 1 文献中.

  • Hengst U  et al. Functional and selective RNA interference in developing axons and growth cones. J Neurosci 26:5727-32 (2006). PubMed: 16723529

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

CLIP abreview for Anti-Argonaute 3 / eIF2C 3 antibody

Inconclusive
Abreviews
Abreviews
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A dynabeads
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 21 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Argonaute 3 / eIF2C 3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Lynn Dong

Verified customer

提交于 Feb 28 2012

Western blot abreview for Anti-Argonaute 3 / eIF2C 3 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human umbilical vein endothelial cells)
Loading amount
50 µg
Specification
human umbilical vein endothelial cells
Gel Running Conditions
Reduced Denaturing (SDS-PAGE 8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 31 2010

Immunocytochemistry/ Immunofluorescence abreview for Anti-Argonaute 3 / eIF2C 3 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (dissociated dorsal root ganglia)
Specification
dissociated dorsal root ganglia
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 07 2006

Western blot abreview for Anti-Argonaute 3 / eIF2C 3 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (dorsal root ganglia explants)
Loading amount
50 µg
Specification
dorsal root ganglia explants
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 28 2006

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