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Anti-Agmatine抗体(ab62667)

Submit a review Q&A (4)

Key features and details

  • Chicken polyclonal to Agmatine
  • Suitable for: ICC
  • Reacts with: Species independent
  • Isotype: IgY

概述

  • 产品名称

    Anti-Agmatine抗体

  • 描述

    鸡多克隆抗体to Agmatine

  • 宿主

    Chicken

  • 特异性

    Fixed tissue cross-reactivity tested with known targets at recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, gamma-aminobutyrate, L-citrulline, L-cysteine, D/L-glutamate, D/L-arginine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine.

  • 经测试应用

    适用于: ICCmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Agmatine cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab62667于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
说明
应用说明
ICC: 1/100. Excitation mapping by LM immunocytochemistry. True dilution at user dilution: 1/2000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.1% using HPI.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 相关性

    Agmatine, a product of arginine metabolism is an endogenous amine acting at imidazoline receptors with a putative role in nociception at the spinal level. Agmatine potentiates morphine analgesia and blocks symptoms of naloxone-precipitated morphine withdrawal in rats.

  • 别名

    • (4 Aminobutyl)guanidine antibody
    • 1 Amino 4 guanidobutane antibody
    • 2 (4 Aminobutyl)guanidine antibody

文献 (0)

发表研究结果有使用 ab62667？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab62667 尚未被引用在任何文献中。

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Question

Okay. Could you please send a replacement vial of the same lot?

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

The item is currently out of stock but is expected to be delivered around Aug. 9. Please note that the lot number of this new stock is not known at this time. To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Question

I have spoken with my boss about this. If I am sent the same lot, will there still be an opportunity for a refund if it does not work? We would initially prefer another lot or a refund if that is the case. Thanks

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Answer

Thanks for your reply.

You are correct that our Abpromise guarantee would apply to a vial of the same lot of ab62667. That is, if it doesn't work as noted on the online product datasheet, we are happy to offer a replacement, credit or refund as long as you follow up with us within 6 months of receiving the product.

Would you like me to send you a vial of this same lot of ab62267?

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Question

Thank you for you response. I would like to have a replacement antibody from a different lot. The reference number was: Please let me know when this replacement item is shipped. Thank you!

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Answer

Thanks for your email.

At this time we just have a few vials left of ab62667 and they are of the same lot that gave you trouble. We should be getting some new stock in within 1-2 weeks however I do not know if it will be more of the same lot or a new lot.

So I can send you a vial of the same lot, send you a vial of our other anti-Agmantine antibody ab37001, or perhaps you'd like to wait until we get some more stock in of ab62667?

When you have a chance please let me know how you wish to proceed.

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Question

Inquiry: I can not get this antibody to work. I am using this antibody to detect the presence of agmatine in retinal tissue following the activation of glutamate receptors in the presence of exogenous agmatine in the buffer. This is the same protocol as developed by Marc, R et al 2005. I use the recommended fixation with glutaraldehyde (0.1%) and paraformaldehyde (4%). Furthermore this same tissue labels with the polyclonal rabbit agmatine that we have leading us to believe that this is an antibody issue. We've tried both 1:100 and 1:1000 concentrations. This antibody was purchased 1 month ago. I can provide images at your request.

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Answer

Thanks for your email and the complete description of the issues you are experiencing with ab62667.

I agree the vial of ab62667 you received is not working as expected. I would be happy to offer a replacement of any primary antibody, credit or refund. So I can process your request, please provide the PO number or Abcam order number associated with this order.

Thanks in advance for the additional information.

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