Anti-Aggrecan ARGxx抗体[BC-3] (ab3773)
Key features and details
- Mouse monoclonal [BC-3] to Aggrecan ARGxx
- Suitable for: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WB
- Reacts with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-Aggrecan ARGxx抗体[BC-3] -
描述
小鼠单克隆抗体[BC-3] to Aggrecan ARGxx -
宿主
Mouse -
特异性
Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Samples need to be deglycosylated for epitope recognition (see notes). -
经测试应用
适用于: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WBmore details -
种属反应性
与反应: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
不与反应: Mouse -
免疫原
Synthetic peptide corresponding to Aggrecan ARGxx.
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常规说明
Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 3.16% Tris HCl -
Concentration information loading...
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纯度
Tissue culture supernatant -
Primary antibody说明
Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease. -
克隆
单克隆 -
克隆编号
BC-3 -
骨髓瘤
x63-Ag8.653 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration. PubMed: 24312401
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ELISA | (1) |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration. PubMed: 19932675
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IHC-Fr |
Use at an assay dependent concentration.
This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections. |
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IHC-P |
Use at an assay dependent concentration.
This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections. |
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WB |
Use at an assay dependent concentration.
Detects a variety of epitopes between 50 and 250 kDa. |
说明 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 24312401 |
ELISA
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 19932675 |
IHC-Fr
Use at an assay dependent concentration. This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections. |
IHC-P
Use at an assay dependent concentration. This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections. |
WB
Use at an assay dependent concentration. Detects a variety of epitopes between 50 and 250 kDa. |
靶标
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相关性
Aggrecan has been detected in neural precursor cells (neurospheres; Kabos et al, 2004) During differentiation, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 280985 Cow
- Entrez Gene: 403828 Dog
- Entrez Gene: 176 Human
- Entrez Gene: 397255 Pig
- Entrez Gene: 58968 Rat
- Omim: 155760 Human
- SwissProt: P13608 Cow
- SwissProt: Q28343 Dog
see all -
别名
- AGC1 antibody
- Aggrecan 1 antibody
- Aggrecan core protein antibody
see all
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (70)
ab3773 被引用在 70 文献中.
- Li XC et al. M2 macrophage-conditioned medium inhibits intervertebral disc degeneration in a tumor necrosis factor-α-rich environment. J Orthop Res 40:2488-2501 (2022). PubMed: 35170802
- Boneski PK et al. Abcc6 Null Mice-a Model for Mineralization Disorder PXE Shows Vertebral Osteopenia Without Enhanced Intervertebral Disc Calcification With Aging. Front Cell Dev Biol 10:823249 (2022). PubMed: 35186933
- Du X et al. Circular RNA hsa_circ_0083756 promotes intervertebral disc degeneration by sponging miR-558 and regulating TREM1 expression. Cell Prolif 55:e13205 (2022). PubMed: 35187741
- Aidos L et al. Meniscus Matrix Structural and Biomechanical Evaluation: Age-Dependent Properties in a Swine Model. Bioengineering (Basel) 9:N/A (2022). PubMed: 35324808
- Miyazaki K et al. Protective Effects of Growth Differentiation Factor-6 on the Intervertebral Disc: An In Vitro and In Vivo Study. Cells 11:N/A (2022). PubMed: 35406739