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Neuroscience Cell Type Marker Neural Stem Cell marker

Anti-Aggrecan ARGxx抗体[BC-3] (ab3773)

  • Datasheet
Reviews (1)Q&A (20)References (53)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [BC-3] to Aggrecan ARGxx
  • Suitable for: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WB
  • Reacts with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Aggrecan ARGxx抗体[BC-3]
  • 描述

    小鼠单克隆抗体[BC-3] to Aggrecan ARGxx
  • 宿主

    Mouse
  • 特异性

    Recognises the aggrecanase (ADAMTS-1, -4 & -5)-generated N-terminal neoepitope ARG after cleavage between amino acids EGE and ARG within the interglobular domain of aggrecanase-catabolised aggrecan (Human aggrecan sequence enumeration). This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Samples need to be deglycosylated for epitope recognition (see notes).
  • 经测试应用

    适用于: ICC/IF, ELISA, Sandwich ELISA, IHC-Fr, IHC-P, WBmore details
  • 种属反应性

    与反应: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human, Pig
    不与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Aggrecan ARGxx.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Diluted Technomouse culture supernatant.


    Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: 3.16% Tris HCl
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • Primary antibody说明

    Aggrecan degradation products containing this neoepitope are rapidly released from the tissue in model explant culture systems and are also present in the synovial fluids of patients with degenerative joint disease.
  • 克隆

    单克隆
  • 克隆编号

    BC-3
  • 骨髓瘤

    x63-Ag8.653
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Aggrecan
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Recombinant Human Aggrecan protein (ab114254)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3773于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration. PubMed: 24312401
ELISA (1)
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 19932675
IHC-Fr
Use at an assay dependent concentration.

This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections.

IHC-P
Use at an assay dependent concentration.

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections.

WB
Use at an assay dependent concentration.

Detects a variety of epitopes between 50 and 250 kDa.

说明
ICC/IF
Use at an assay dependent concentration. PubMed: 24312401
ELISA
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 19932675
IHC-Fr
Use at an assay dependent concentration.

This antibody should work in IHC on alcohol-fixed frozen sections or un-fixed snap-frozen sections.

IHC-P
Use at an assay dependent concentration.

This antibody should work in IHC on formalin- or paraformaldehyde-fixed paraffin embedded sections.

WB
Use at an assay dependent concentration.

Detects a variety of epitopes between 50 and 250 kDa.

靶标

  • 相关性

    Aggrecan has been detected in neural precursor cells (neurospheres; Kabos et al, 2004) During differentiation, neurospheres downregulate Chondroitin sulfate proteoglycans (CSPGs). Proliferating neural precursors synthesize lecticans, including aggrecan, which are downregulated with differentiation; suggesting a link between CSPGs and CNS precursor biology.
  • 细胞定位

    Secreted
  • 数据库链接

    • Entrez Gene: 280985 Cow
    • Entrez Gene: 403828 Dog
    • Entrez Gene: 176 Human
    • Entrez Gene: 397255 Pig
    • Entrez Gene: 58968 Rat
    • Omim: 155760 Human
    • SwissProt: P13608 Cow
    • SwissProt: Q28343 Dog
    • SwissProt: P16112 Human
    • SwissProt: P07897 Rat
    see all
  • 别名

    • AGC1 antibody
    • Aggrecan 1 antibody
    • Aggrecan core protein antibody
    • CSPCP antibody
    • CSPG1 antibody
    • MSK16 antibody
    see all

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (53)

发表研究结果有使用 ab3773?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3773 被引用在 53 文献中.

  • Park SS  et al. Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex. BMC Neurosci 21:16 (2020). PubMed: 32334536
  • Novais EJ  et al. Comparison of inbred mouse strains shows diverse phenotypic outcomes of intervertebral disc aging. Aging Cell 19:e13148 (2020). PubMed: 32319726
  • Cui S & Zhang L circ_001653 Silencing Promotes the Proliferation and ECM Synthesis of NPCs in IDD by Downregulating miR-486-3p-Mediated CEMIP. Mol Ther Nucleic Acids 20:385-399 (2020). PubMed: 32203911
  • Sirong S  et al. Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis. Bone Res 8:6 (2020). PubMed: 32047705
  • Tessier S  et al. Arp2/3 inactivation causes intervertebral disc and cartilage degeneration with dysregulated TonEBP-mediated osmoadaptation. JCI Insight 5:N/A (2020). PubMed: 31961823
View all Publications for this product

客户评价及客户问答

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1-10 of 21 Abreviews or Q&A

ELISA abreview for Anti-Aggrecan ARGxx antibody [BC-3]

Excellent
Abreviews
Abreviews
Application
ELISA
Sample
Human Recombinant protein (Part of aggrecan peptide 41AA)
Specification
Part of aggrecan peptide 41AA
Type
Indirect
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 29 2006

Question

Ich habe 2 Fragen zu ihren Antikörpern es geht um die Antikörper ab3777 Aggrecan xxPen antibody (BC-4) mit der lot Nummer: 204316 und um den BC-3 Antikörper ab3773 lot Nummer 194564.


Frage ich würde gerne wissen in was für einer Konzentration liegt der Antikörper vor ?

Und gibt es zufällig eine Immunglobulin Konzentration ?







Read More

Abcam community

Verified customer

Asked on Feb 27 2013

Answer



Nicht aufgereinigte Antikörper wie Zellkulturüberstand, Vollserum oder Aszites haben keine determinierte Konzentration und können stark in Ihrer Konzentration schwanken.

Wenn die spezifische Antikörperkonzentration unbekannt ist, werfen Sie einen Blick auf die „Ungefähre Konzentration“ in unserer Tabelle in den FAQs, um den Wert abschätzen zu können. Bitte beachten Sie, dass diese Konzentrationen als Richtlinien benutzt werden sollten und die verwendeten Antikörperkonzentrationen daher Ihren Resultaten angepasst werden müssen.

Laut der Tabelle ist die ungefähre Immunglobulin- Konzentration 1 - 3 mg/ml.

Read More

Abcam Scientific Support

回复于 Feb 27 2013

Question

TSC - was cultured in serum?
is the Ab available in purified form?

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Abcam community

Verified customer

Asked on Mar 12 2012

Answer

TSC - was cultured in serum?
A: Serum was used during culture.

is the Ab available in purified form?
A: We do not routinely purify the antibody and so cannot give an answer to the second question. However, it could be purified.

I hope this is helpful. Please contact us again if you have any further questions.

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Abcam Scientific Support

回复于 Mar 12 2012

Question

Dear technical team, Our customer would like to purchase 5 antibodies from abcam and wants to check control tissue. Pleaselet me knowabcam's product information. ab66025 ab102711 ab3773 ab2426 ab58632 Best regards,

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Abcam community

Verified customer

Asked on Jan 06 2012

Answer

Thank you for your inquiry. Please find a list of possible positive controls below: ab2426: skin, lymph nodes Click here (or use the following: https://www.abcam.com/PCNA-antibody-ab2426.html). ab3773: cartilage samples Click here (or use the following: https://www.abcam.com/Aggrecan-ARGxx-antibody-BC-3-ab3773.html). ab58632: Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Click here (or use the following: https://www.abcam.com/Collagen-X-antibody-ab58632.html). ab66025: The recommended positive control tissues are rat head tissue, rat embryo tissue, human osteosarcoma tissue, human glioma tissue, human intestinal cancer tissue. Click here (or use the following: https://www.abcam.com/N-Cadherin-antibody-NC-17-ab66025.html). ab102711: Specifically expressed in osteoblasts Click here (or use the following: https://www.abcam.com/RUNX2-antibody-ab102711.html). I hope this information is helpful for your customer. I wish them good luck with their experiments.

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Abcam Scientific Support

回复于 Jan 06 2012

Question

Which antibody is most suitable for detecting full length aggregan protein in goat tissue?

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Abcam community

Verified customer

Asked on Jun 05 2007

Answer

I have been informed of the following information which I hope will help you: Ab3773: this should be reactive with goat since this antibody recognises a sequence ARG... that is conserved in all species Guinea Pig/rat to human but not mouse for some reason where the sequence is ALG.... This antibody will not recognise the sequence ...ARG.. if it is in the non-cleaved intact aggrecan protein core; i.e. it will only recognise the aggrecanase generated neoepitope ARG... Ab3778: this antibody recognises a linear sequence ..EPEEP... that is present in the IGD protein core of human and bovine aggrecan but not pig and several other animals; it seems it is not present in goat protein after BLASTing the sequence in goat. This antibody recognises the linear epitope ...EPEEP... in both intact and matrix protease degraded aggrecan from human and bovine cartilage. Please do not hesitate to contact me if I can be of further help,

Read More

Abcam Scientific Support

回复于 Jun 05 2007

Question

I want to buy a mouse monoclonal antibody to aggrecan ARGxxx Cat no. ab3773. I wang to use it for immunohistochemistry for aggrecan of rattus chondrocyte. I want to konw whether I must treat the chondrocytes with digestion mixture ( Chondroitnase ABC Sigma, Keratanase Seikagaku and keratanase II Seikagaku ) as recommended before immunohistochemistry or western-blot.

Read More

Abcam community

Verified customer

Asked on May 24 2007

Answer

Thank you for your enquiry. The following reference gives details of the protocol used with this antibody in immunohistochemistry: Roberts S et al. Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc. Spine 25:3005-13 (2000). PubMed: 11145811 I have added the relevant protocol to our online datasheet for your convenience and include below the following publications which have all used this product. This product has been used in: Karsdal MA et al. Induction of increased cAMP levels in articular chondrocytes blocks matrix metalloproteinase-mediated cartilage degradation, but not aggrecanase-mediated cartilage degradation. Arthritis Rheum 56:1549-58 (2007). PubMed: 17469134 Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). PubMed: 12009333 Flannery CR et al. Autocatalytic cleavage of ADAMTS-4 (Aggrecanase-1) reveals multiple glycosaminoglycan-binding sites. J Biol Chem 277:42775-80 (2002). PubMed: 12202483 Malfait AM et al. Inhibition of ADAM-TS4 and ADAM-TS5 prevents aggrecan degradation in osteoarthritic cartilage. J Biol Chem 277:22201-8 (2002). PubMed: 11956193 Roberts S et al. Matrix metalloproteinases and aggrecanase: their role in disorders of the human intervertebral disc. Spine 25:3005-13 (2000). PubMed: 11145811 Caterson B et al. Mechanisms involved in cartilage proteoglycan catabolism. Matrix Biol 19:333-44 (2000). PubMed: 10963994 Billington CJ et al. An aggrecan-degrading activity associated with chondrocyte membranes. Biochem J 336 ( Pt 1):207-12 (1998). PubMed: 9806902 Buttle DJ et al. "Aggrecanase" activity is implicated in tumour necrosis factor alpha mediated cartilage aggrecan breakdown but is not detected by an in vitro assay. Mol Pathol 50:153-9 (1997). PubMed: 9292151 Hughes CE et al. Monoclonal antibodies that specifically recognize neoepitope sequences generated by 'aggrecanase' and matrix metalloproteinase cleavage of aggrecan: application to catabolism in situ and in vitro. Biochem J 305 ( Pt 3):799-804 (1995). PubMed: 7531436 This clone has been used in: Molecular Approaches to Osteoarthritis Research. Abstracts of the Kennedy Institute of Rheumatology Symposium, London, United Kingdom, April 18-20, 2004. Scand J Rheumatol Suppl :1-41 (2005). PubMed: 15864865 Roberts S et al. Matrix turnover in human cartilage repair tissue in autologous chondrocyte implantation. Arthritis Rheum 44:2586-98 (2001). PubMed: 11710715 Aplin JD et al. Human endometrial MUC1 carries keratan sulfate: characteristic glycoforms in the luminal epithelium at receptivity. Glycobiology 8:269-76 (1998). PubMed: 9451036

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Abcam Scientific Support

回复于 May 24 2007

Question

Can you tell me the molecular weigth for the full-length protein and also the protein after it has been degraded? I am using rabbit and guinea pig tissue.

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Abcam community

Verified customer

Asked on Nov 03 2006

Answer

Thank you for your patience. I can see that my colleague has also written to you and referred you to the paper in which the antibody was tested, i.e. Little CB et al., Matrix Biol 21:271-88 (2002). In summary the antibody detects a variety of epitopes between 50 and 250 kDa. I also contacted the originator of the antibody. Using ab3773 in Western blots can provide a wide range (of MW) of bands that are immuno-positive for the BC-3 neoepitope (i.e. ARG... as a result of IGD aggrecanase cleavage). Thus, if you are looking at media from cartilage explant cultures exposed to cytokines such as IL-1 you will find a wide range of MW product usually from 50kD - 250kD. However, if you use cartilage from young animals this can give mainly large MW bands and if the aggrecanase activity has been low you may see only bands at 40/50 - 90kD. I hope this additional information helps you.

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Abcam Scientific Support

回复于 Nov 07 2006

Question

Can you let me know the detected band size using ab3773 for synovial fluid Western blot?

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Abcam community

Verified customer

Asked on Nov 03 2006

Answer

The antibody ab3773 was tested in western blot analysis on proteoglycan metabolites released into the culture media and extracted from the cartilage matrix in cultures of bovine nasal cartilage maintained for 21 days in the paper Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). In figure 2 you will be able to see that the antibody detected bands of different sizes and thickness depending on the absence of 50 ng/ml interleukin-1 alone (IL-1), and IL-1 plus an inhibitor of MMPs at a high dose (1 mM) or a low dose (40 nM). In summary the antibody detects a variety of epitopes between 50 and 250 kDa. Because of copyright I am not able to forward the image of the figure to you or add it to the datasheet, but I hope you will be able to access the paper to see the banding pattern observed with the antibody. I hope this information will help you,

Read More

Abcam Scientific Support

回复于 Nov 06 2006

Question

With regards to the poor immunostaining of aggrecan by ab3773 product, I would like to enquire on the following. I am interested in immunostaining of pig tissues, frozen sections ( Antibodies raised in mouse or rabbit ). I am considering the 2 other abcam aggrecan antibodies ab3776 and ab3777. Hence I would like to confirm with you on the effectiveness of these antibodies for my application. Moreover I would like to have the protocols which are tested to be effective when these antibodies are used.

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Abcam community

Verified customer

Asked on May 08 2006

Answer

Thank you for getting back in touch with me. I have been in touch with the originator of this antibody. They have provided me with some further details. They mention that both ab3773 & ab3776 are not usually used for IHC studies of articular cartilage because aggrecan catabolites with these (aggrecanase ab3773 or ab3776) generated neoepitopes are not retained within the cartilage; i.e. They are usually released into the synovial fluid or media if cultures are being examined. It may be possible that some ab3773 & ab3776 positive catabolites may be retained in tendon or intervertebral disc tissue; however, we have not examined this. If this was to be undertaken on these non-cartilagenous tissues a dilution of 1:100 should suffice. Regarding using ab3777 (anti ...PEN) on cartilage; a dilution of 1:100 (same as Western blots) should suffice. If they are looking for aggrecanase-generated neoepitope then they should use ab3775 (anti- ...EGE). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

回复于 May 11 2006

Question

In the technical data sheet, it is as follows : Notes Samples must be deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10µg S-GAG of non-deglycosylated aggrecan for optimal epitope recognition. See Little et al. and Caterson et al. Furthermore, controls were done whereby no deglycosylation was carried out and the results were negative. Those with deglycosylation were faint. These were cartilage sections which were meant to be positive controls. The following dilutions were tried : 20, 50 and 100 times. Thank you.

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Abcam community

Verified customer

Asked on Apr 26 2006

Answer

Thank you for getting back to me and highlighting these details on our datasheet. I can now appreciate the deglycosylation that you have performed in line with the following publication. Little CB et al. Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation. Matrix Biol 21:271-88 (2002). PubMed: 12009333. I am sorry to heat that you have been having difficulties with this antibody. I would like to offer you credit against your initial purchase provided it was made within the past 90 days. If this is the case please e-mail me details of the order number and date of purchase.

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Abcam Scientific Support

回复于 May 02 2006

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