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Tags & Cell Markers Epitope Tags T7 Tag

Agarose Anti-T7 tag®抗体(ab1230)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (3)

Product price, shipping and contact information

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Key features and details

  • Agarose Goat polyclonal to T7 tag®
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

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概述

  • 产品名称

    Agarose Anti-T7 tag®抗体
    参阅全部 T7 tag® 一抗
  • 描述

    Agarose山羊多克隆抗体to T7 tag®
  • 宿主

    Goat
  • 偶联物

    Agarose
  • 经测试应用

    适用于: IPmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Goats were immunized with

    MASMTGGQQMG

    (T7) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    T7-TAG® is a trademark of EMD Biosciences, Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • T7 Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Agarose Conjugates

相关产品

  • Isotype control

    • Agarose Goat IgG, polyclonal - Isotype Control (ab104155)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1230于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at an assay dependent concentration.
说明
IP
Use at an assay dependent concentration.

靶标

  • 相关性

    The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • 别名

    • 10 antibody
    • Major capsid protein 10A antibody
    • Major capsid protein antibody
    • Minor capsid protein 10B antibody
    • T7 epitope tag antibody
    see all

实验方案

  • Immunoprecipitation protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (3)

发表研究结果有使用 ab1230?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1230 被引用在 3 文献中.

  • Jiang T  et al. Plastid chaperone HSP90C guides precursor proteins to the SEC translocase for thylakoid transport. J Exp Bot 71:7073-7087 (2020). PubMed: 32853383
  • Piccinni E  et al. Direct interaction of Gas41 and Myc encoded by amplified genes in nervous system tumours. Acta Biochim Pol 58:529-34 (2011). IP . PubMed: 22068108
  • MacPherson MJ  et al. The CTCF insulator protein is posttranslationally modified by SUMO. Mol Cell Biol 29:714-25 (2009). IP . PubMed: 19029252

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Hi,
I would like to check if this product, Anti-T7 tag antibody (Agarose) (ref no.ab1230), is used for purification of T7-tagged protein. If so, is there any protocol which I can refer to. In addition, what is the cost and availability of this product?

Read More

Abcam community

Verified customer

Asked on Apr 24 2012

Answer

Thank you for contacting us.

Indeed, we can confirm that Agarose-conjugated anti-T7 tag® antibodyab1230 can be used to purify (or pull-down) T7-tagged proteins, as it has been tested and is guaranteed to work in immunoprecipitation (IP).

For a protocol you can refer to the publication by MacPherson who used thisproduct(see attached, and datasheet). For IP protocols in general our protocols website might be of interest for you: https://www.abcam.com/index.html?pageconfig=popular_protocols

We currently have this product in stock. For further details on pricing and shipping time please contact our distributor in your country. By choosing your country in the top right corner of our website the contact details will be shown on each datasheet, e.g. for Australia it is currently

Sapphire Bioscience Pty Ltd
126 Cope Street
Waterloo - NSW
2017
Australia
tel +61296982022
Fax +61296981022
Website http://www.sapphirebioscience.com
Email mailto:sales@sapphirebioscience.com
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Apr 24 2012

Question

DESCRIPTION OF THE PROBLEM High background and non-specific protein binding to agarose SAMPLE total cell lysate PRIMARY ANTIBODY T7 antibody from Novagen DETECTION METHOD ECL plus POSITIVE AND NEGATIVE CONTROLS USED T7 antibody ANTIBODY STORAGE CONDITIONS 4? SAMPLE PREPARATION RIPA buffer MATRIX USED .. DETERGENT 1mg protein /60ul agarose AMOUNT OF PROTEIN LOADED Reducing gel(SDS-PAGE) 12% ELECTROPHORESIS/GEL CONDITIONS 80V, 2hr SECONDARY ANTIBODY Amersham/Mouse/1:2000/1hr HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Preclearing with various agarose

Read More

Abcam community

Verified customer

Asked on Feb 05 2007

Answer

Thank you for contacting us for technical support with ab1230 on behalf of your customer. Unfortunately it is very hard for me to understand the customer's protocol and results as limited information was provided. Can you please explain what the different lanes are: -what is the positive control? -what is the difference between the two membrane images? (the lanes IP and Total cell lysate don't look the same at all between the two images, I am very confused) -what does "Rx" mean? I would like also to know more protocol information please: -how was the T7 antibody used in WB used: dilution, buffer, incubation time. Is there a positive control for this antibody? -what protease inhibitors were used in the RIPA buffer? -how long was the lysate mixed with the antibody/agarose slurry? -how were the agarose beads uncoupled from the antibody? -what blocking buffer was used: for the beads and the membrane? The more information you can provide the better it will be for me to assist your customer, thank you in advance,

Read More

Abcam Scientific Support

回复于 Feb 05 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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