为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Tags & Cell Markers Epitope Tags Myc Tag

Agarose Anti-Myc tag抗体(ab1253)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (12)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Agarose Goat polyclonal to Myc tag
  • Suitable for: IP
  • Reacts with: Species independent
  • Conjugation: Agarose
  • Isotype: IgG

你可能会感兴趣

一抗
Product image
Anti-OPA1 antibody [EPR11057(B)] (ab157457)
一抗
Product image
Anti-OPA1 antibody [EPR11057(B)] - BSA and Azide free (ab240143)
一抗
Product image
Anti-Mitofusin 2 antibody [6A8] (ab56889)

查看更多相关产品

概述

  • 产品名称

    Agarose Anti-Myc tag抗体
    参阅全部 Myc tag 一抗
  • 描述

    Agarose山羊多克隆抗体to Myc tag
  • 宿主

    Goat
  • 偶联物

    Agarose
  • 经测试应用

    适用于: IPmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Full length native protein (purified) corresponding to Human Myc tag conjugated to keyhole limpet haemocyanin.
    Database link: P01106
    (Peptide available as ab13837)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Affinity purified antibodies were coupled to agarose beads using a cyanogen bromide method.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C.
  • 存储溶液

    pH: 6.8
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Agarose Conjugates

相关产品

  • Isotype control

    • Agarose Goat IgG, polyclonal - Isotype Control (ab104155)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1253于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP (2)
Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.
说明
IP
Use a concentration of 20 - 40 µg/ml. Use at a concentration of 20 - 40 µg/ml. Use at a concentration of 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
  • 细胞定位

    Nuclear
  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

实验方案

  • Immunoprecipitation protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (12)

发表研究结果有使用 ab1253?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1253 被引用在 12 文献中.

  • Strutt H & Strutt D DAnkrd49 and Bdbt act via Casein kinase Ie to regulate planar polarity in Drosophila. PLoS Genet 16:e1008820 (2020). PubMed: 32750048
  • Huang Z  et al. PTPN2 regulates the activation of KRAS and plays a critical role in proliferation and survival of KRAS-driven cancer cells. J Biol Chem 295:18343-18354 (2020). PubMed: 33122197
  • Bucher ML  et al. Acquired dysregulation of dopamine homeostasis reproduces features of Parkinson's disease. NPJ Parkinsons Dis 6:34 (2020). PubMed: 33298952
  • Katoh I  et al. C-terminal a Domain of p63 Binds to p300 to Coactivate ß-Catenin. Neoplasia 21:494-503 (2019). PubMed: 30986748
  • Cai L  et al. Identification of a genetic interaction between the tumor suppressor EAF2 and the retinoblastoma protein (Rb) signaling pathway in C. elegans and prostate cancer cells. Biochem Biophys Res Commun 447:292-8 (2014). PubMed: 24727455
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Myc tag antibody (Agarose)

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Trypanosoma brucei Cell lysate - other (Abcam beads (ab1253) Anti-myc developed in goat) 1)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
2.5 µg
Specification
Abcam beads (ab1253) Anti-myc developed in goat) 1
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 17 2020

Immunoprecipitation abreview for Anti-Myc tag antibody (Agarose)

Excellent
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HEK 293T)
Total protein in input
25 µg
Specification
HEK 293T
Immuno-precipitation step
Other - Pre-bound agarose
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Jonathan Rud

Verified customer

提交于 Dec 28 2009

Immunoprecipitation abreview for Anti-Myc tag antibody (Agarose)

Average
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Immuno-precipitation step
Other
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 03 2005

Question

Is there an IP protocol that should be used with this antibody? Specifically, customer would like to know if the beads should be washed prior to use and if so, with what buffer?

Read More

Abcam community

Verified customer

Asked on Aug 08 2005

Answer

Thank you for your enquiry. You don't need to wash the beads prior to using, and perform the IP at 4C. Also, use 15-25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract. Below is a general IP protocol that the originator of this antibody sent that you can use as a guideline. If you have any additional questions, please contact us again. Immunoprecipitation Protocol For use with Antibodies Immobilized on Agarose: A. To microcentrifuge tube add: 1) 15 to 25 ul of slurry of agarose immobilized Ab (3.75 to 6.125 ug Ab) 2)500 ul 2x Stringent IP buffer*: 100 mM Tris-HCl (pH 8.0) 1000 mM Nacl Chelating agents: 2 mM EDTA and/or 2 mM EDTA Detergents: 2 % IGEPAL CA-630 (replacement for NP40) 0.2 % SDS 2 % Deoxycholate Protease Inhibitors: 20 ug/ml Aprotinin 20 ug/ml Leupeptin 2 mM PMSF or 10 ug/ml Pepstatin A or 8 mM AEBSF Phosphatase Inhibitors – If Necessary: 200 mM NaF 2 mM Na3VO4 50 mM ?-Glycerophosphate 3)Cell lysate (0.1 to 1.0 mg total protein) 4)diH2O to a total volume of 1 ml B. Vortex and incubate for between 1 and 24 h at 4 deg. Turning tubes on a rotating platform wheel allows continuous mixing. C. Centrifuge (16,000 x g, 3 to 5 min), aspirate and discard supernatant D. Wash pellet in 1x IP buffer 3 to 6 times. Washing consists of resuspending pellet, centrifuging (16,000 x g, 3 to 5 min), aspirating and discarding supernatant. E. After last wash, resuspend pellet in 30 ul 2x Electrophoresis Sample Buffer, boil 5 min. F. Centrifuge, load supernatant onto SDS-PAGE gel, electroblot onto PVDF membrane and perform Western Blot Analysis. *Buffers for Immunoprecipitation Assays For analysis of a single protein without associated proteins, the use of high ionic strength, strong detergents, and rigorous washing of the immunoprecipitate are recommended. One suitable buffer is the Strigent IP buffer outlined above. For co-precipitation of associated proteins, the use of near physiological ionic strength and gentle detergents is recommended. One suitable buffer is the Lenient IP buffer outlined below. The buffers outlined represent two extremes. The optimal buffer for ones given application must be empirically defined. This buffer may be of intermediate ionic strength and/or detergent composition. 1x Lenient IP buffer: 50 mM Tris-HCl (pH 8.0) 100 mM Nacl Chelating agents: 1 mM EDTA and/or 1 mM EGTA Detergents: 0.5 % IGEPAL CA-630 (replacement for Nonidet P40) Protease Inhibitors: 10 ug/ml Aprotinin 10 ug/ml Leupeptin 1 mM PMSF or 5 ug/ml Pepstatin A or 4 mM AEBSF Phosphatase Inhibitors – If Necessary: 100 mM NaF 1 mM Na3VO4 50 mM ?-Glycerophosphate

Read More

Abcam Scientific Support

回复于 Aug 08 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有