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AB251346

重组Anti-AG-3抗体[EPR19606] - BSA and Azide free

Anti-AG-3 antibody [EPR19606] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal AG-3 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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BCMP11, PDIA18, UNQ642/PRO1272, AGR3, Anterior gradient protein 3, AG-3, AG3, hAG-3, Anterior gradient 3 homolog, Breast cancer membrane protein 11

3 Images
Flow Cytometry (Intracellular) - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (AB251346)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (AB251346)

This data was developed using ab201464, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (AB251346)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (AB251346)

This data was developed using ab201464, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AG-3 with ab201464 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab201464 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Western blot - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (AB251346)
  • WB

Supplier Data

Western blot - Anti-AG-3 antibody [EPR19606] - BSA and Azide free (AB251346)

This data was developed using ab201464, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lanes 1 and 5 : 15 seconds; Lanes 2-4 : 3 minutes.

Lanes 1 and 5:

Western blot - Anti-AG-3 antibody [EPR19606] (<a href='/products/primary-antibodies/ag-3-antibody-epr19606-ab201464'>ab201464</a>) at 1/2000 dilution

Lane 2:

Western blot - Anti-AG-3 antibody [EPR19606] (<a href='/products/primary-antibodies/ag-3-antibody-epr19606-ab201464'>ab201464</a>) at 1/10000 dilution

Lanes 3 - 4:

Western blot - Anti-AG-3 antibody [EPR19606] (<a href='/products/primary-antibodies/ag-3-antibody-epr19606-ab201464'>ab201464</a>) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Lane 2:

T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg

Lane 3:

Human colon cancer tissue lysate at 10 µg

Lane 4:

Human breast cancer tissue lysate at 10 µg

Lane 5:

Human ovary cancer tissue lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 5:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

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不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19606

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Primary Antibodies

AB55863

PE Anti-DR5 antibody [DR5-01-1]

Flow Cytometry - PE Anti-DR5 antibody [DR5-01-1] (AB55863)

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

ab251346 is the carrier-free version of ab201464.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AG-3 also known as protein AG is a protein of about 45 kDa. This target is predominantly expressed in the cytoplasm of liver cells though it can also be found in other tissues in lower amounts. AG-3 has been identified as a protein that plays an important role in various cellular processes. Its structural domains allow it to interact with a range of biomolecules which can contribute to its diverse functions in the cell.
Biological function summary

AG-3 enables essential cellular processes such as signal transduction and metabolic regulation. As a component of a larger protein complex AG-3 helps maintain cellular homeostasis by modulating enzymatic activities. Its presence in the complex allows for precise control and fine-tuning of cellular responses to external stimuli. Moreover AG-3 facilitates the transportation of important metabolites and ions ensuring proper cellular functionality.

Pathways

AG-3 significantly contributes to the MAPK signaling pathway and the insulin signaling pathway. Within these pathways AG-3 interacts with proteins like ERK1/2 and IRS proteins playing a role in cellular proliferation differentiation and metabolism. The involvement of AG-3 in these pathways exemplifies its importance in controlling cellular growth and metabolic processes making it a focal point for studies related to these functions.

AG-3 is linked to conditions like type 2 diabetes and liver diseases. Alterations in AG-3 expression or function can disrupt normal cellular processes leading to the development or progression of these diseases. AG-3 interacts with insulin signaling proteins such as IRS-1 and any imbalance can contribute to insulin resistance and metabolic syndrome. Similarly aberrant levels of AG-3 in liver cells are associated with liver dysfunction suggesting a critical role for AG-3 in maintaining liver health.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Required for calcium-mediated regulation of ciliary beat frequency and mucociliary clearance in the airway. Might be involved in the regulation of intracellular calcium in tracheal epithelial cells.
See full target information AGR3
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu
View all publications
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