Anti-AFAF/Equatorin抗体(ab121470)
Key features and details
- Rabbit polyclonal to AFAF/Equatorin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-AFAF/Equatorin抗体
参阅全部 AFAF/Equatorin 一抗 -
描述
兔多克隆抗体to AFAF/Equatorin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human AFAF/Equatorin aa 35-182.
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阳性对照
- Human testis tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121470于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
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| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Involved in acrosome biogenesis. -
组织特异性
Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood. -
细胞定位
Cytoplasmic vesicle > secretory vesicle > acrosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 54586 Human
- SwissProt: Q9NQ60 Human
- Unigene: 163070 Human
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别名
- Acrosome formation-associated factor antibody
- Afaf antibody
- AFAF_HUMAN antibody
see all
图片
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Western blot - Anti-AFAF/Equatorin antibody (ab121470)Lanes 2-3 : Anti-AFAF/Equatorin antibody (ab121470)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing AFAF/Equatorin with a C-terminal myc-DDK tag (~3.1kDa) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AFAF/Equatorin antibody (ab121470)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling AFAF/Equatorin with ab121470. Staining shows strong cytoplasmic positivity in spermatids.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121470 被引用在 1 文献中.
- Luo ZY et al. Ubiquitin Ligase Nrdp1 Controls Autophagy-Associated Acrosome Biogenesis and Mitochondrial Arrangement during Spermiogenesis. Cells 12:N/A (2023). PubMed: 37759433