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Anti-Aequorin抗体(ab9096)

  • Datasheet
Submit a review Q&A (1)References (7)

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Key features and details

  • Rabbit polyclonal to Aequorin
  • Suitable for: ELISA, WB
  • Reacts with: Aequorea victoria
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Aequorin抗体
  • 描述

    兔多克隆抗体to Aequorin
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ELISA, WBmore details
  • 种属反应性

    与反应: Aequorea victoria
  • 免疫原

    Full length native protein (purified) corresponding to Aequorin.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Constituent: Whole serum
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab9096于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000.
说明
ELISA
Use at an assay dependent concentration.
WB
1/1000 - 1/10000.

靶标

  • 相关性

    Aequorin, from the jellyfish Aequoria victoria, catalyses the oxidation of coelenterazine, but only in the presence of Ca2+ ions.
  • 数据库链接

    • SwissProt: P02592 Aequorea victoria
    • SwissProt: P07164 Aequorea victoria
    • 别名

      • Aequorin 1 antibody
      • Aequorin 2 antibody
      • Aequorin1 antibody
      • Aequorin2 antibody

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (7)

    发表研究结果有使用 ab9096?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab9096 被引用在 7 文献中.

    • Lee GM  et al. The Ycx1 protein encoded by the yeast YDL206W gene plays a role in calcium and calcineurin signaling. J Biol Chem 299:104647 (2023). PubMed: 36965615
    • Thor K  et al. The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Nature 585:569-573 (2020). PubMed: 32846426
    • Chan HY  et al. Expression and reconstitution of the bioluminescent Ca(2+) reporter aequorin in human embryonic stem cells, and exploration of the presence of functional IP3 and ryanodine receptors during the early stages of their differentiation into cardiomyocytes. Sci China Life Sci 59:811-24 (2016). PubMed: 27430888
    • Mehlmer N  et al. A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana. J Exp Bot 63:1751-61 (2012). WB . PubMed: 22213817
    • Hamorsky KT  et al. A protein switch sensing system for the quantification of sulfate. Anal Biochem 421:172-80 (2012). PubMed: 22067979
    • Rogers KL  et al. Non-invasive in vivo imaging of calcium signaling in mice. PLoS One 2:e974 (2007). WB . PubMed: 17912353
    • Courchesne WE & Ozturk S Amiodarone induces a caffeine-inhibited, MID1-depedent rise in free cytoplasmic calcium in Saccharomyces cerevisiae. Mol Microbiol 47:223-34 (2003). PubMed: 12492866

    客户评价及客户问答

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    提交评价 提交问题

    Question

    Dear Sir or Madame,
    I am looking for Antibodies against Fluorescent Proteins to be used in Immunohistochemistry. I want to perform tripple-stainings against GFP, dsRed and an additional fluorescent Protein.
    Which additional fluorescent Protein and which of your ABs would you recommend?
    Ideally it can be combined with your Living Color dsred Polyclonal (raised in rabbit, Cat. 632496) and an anti-GFP (raised in chicken) which we are currently using to stain for our constructs containing the dsRed- and GFP-protein.
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 10 2012

    Answer

    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however there are many fluorescent proteins and we are not specialized in this particular field. We donot want to recommend a product that may be unsuitable or not the best choice. I would thereforeencourageyou to consult the latest literature available through PubMed and other resources in order to find the most adapted fluorescent protein to complement your immunohistochemistry.

    Improved blue fluorescent proteins are for example described here:
    http://www.ncbi.nlm.nih.gov/pubmed/17444659

    Additional information source may be ˜
    http://www.scholarpedia.org/article/Fluorescent_proteins
    http://www.nature.com/nature/journal/v450/n7166/abs/nature06293.html
    http://www.conncoll.edu/ccacad/zimmer/GFP-ww/GFP-1.htm


    As antibodies, in addition to all the anti GFP/EGFP/YFP/RFP antibodies,we do have an antibody against the blue fluorescent protein ab32791, which is a mouse monoclonal antibody or the anti aequorin antibody, which is a rabbit polyclonal, ab9096.
    https://www.abcam.com/Enhanced-Blue-Fluorescent-Protein-antibody-3A6-ab32791.html (or use the following: https://www.abcam.com/Enhanced-Blue-Fluorescent-Protein-antibody-3A6-ab32791.html).
    https://www.abcam.com/Aequorin-antibody-ab9096.html (or use the following: https://www.abcam.com/Aequorin-antibody-ab9096.html).

    I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again if you would need some more specific information on any of our products.

    I wish you good success withyour research!

    Read More

    Abcam Scientific Support

    回复于 Apr 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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