重组Anti-ADX抗体[EPR4629] (ab108257)
![Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (ab108257)](https://www.abcam.cn/ps/products/108/ab108257/Images/ab108257-548201-anti-adx-antibody-epr4629-immunocytomety.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (ab108257)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4629] to ADX
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ADX抗体[EPR4629]
参阅全部 ADX 一抗 -
描述
兔单克隆抗体[EPR4629] to ADX -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: A549 cells. WB: A549 whole cell lysate, Mouse and Rat kidney lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4629 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108257于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa).
|
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| ICC/IF |
1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 19 kDa). |
|
ICC/IF
1/100. |
靶标
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功能
Participates in the synthesis of thyroid hormones. Transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. -
序列相似性
Belongs to the adrenodoxin/putidaredoxin family.
Contains 1 2Fe-2S ferredoxin-type domain. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 2230 Human
- Entrez Gene: 14148 Mouse
- Entrez Gene: 29189 Rat
- Omim: 103260 Human
- SwissProt: P10109 Human
- SwissProt: P46656 Mouse
- SwissProt: P24483 Rat
- Unigene: 744 Human
see all -
别名
- Adrenal ferredoxin antibody
- Adrenodoxin antibody
- Adrenodoxin, mitochondrial antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ADX antibody [EPR4629] (ab108257)
Immunocytochemistry/Immunofluorescence analysis of A549 (human lung carcinoma epithelial) cells with ab108257 at a 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Mouse anti-COX IV monoclonal antibody - Mitochondrial Marker was used as a counterstain at a 1/50 dilution followed by ab150115 Alexa Fluor® 647 Goat anti-Mouse secondary antibody used at a 1/1000 dilution.
The negative controls are shown in bottom middle and right hand panels - for negative control 1, ab108257 was used at a dilution of 1/100 followed by Alexa Fluor® 647 Goat anti-Mouse secondary ab150115 at a dilution of 1/1000. For negative control 2, Mouse anti-COX IV monoclonal antibody - Mitochondrial Marker was used at a dilution of 1/50 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at a dilution of 1/1000.
Confocal image showing cytoplasmic staining in A549 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-ADX antibody [EPR4629] (ab108257)](https://www.abcam.cn/ps/products/108/ab108257/Images/ab108257-467557-anti-adx-antibody-epr4629-westernblot-human-mouse-rat.jpg)
Western blot - Anti-ADX antibody [EPR4629] (ab108257)All lanes : Anti-ADX antibody [EPR4629] (ab108257) at 1/1000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat kidney lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 19 kDa -
![Anti-ADX antibody [EPR4629] (ab108257)](https://www.abcam.cn/ps/products/108/ab108257/Images/ab108257-3-benefits-of-recombinant-antibodies.png)
Anti-ADX antibody [EPR4629] (ab108257)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab108257 被引用在 10 文献中.
- Wang L et al. High expression of cuproptosis-related gene FDX1 in relation to good prognosis and immune cells infiltration in colon adenocarcinoma (COAD). J Cancer Res Clin Oncol 149:15-24 (2023). PubMed: 36173462
- Wang W et al. Ferroptosis inducers enhanced cuproptosis induced by copper ionophores in primary liver cancer. J Exp Clin Cancer Res 42:142 (2023). PubMed: 37277863
- Feng J et al. Construction and validation of a novel cuproptosis-mitochondrion prognostic model related with tumor immunity in osteosarcoma. PLoS One 18:e0288180 (2023). PubMed: 37405988
- Quan B et al. LINC02362/hsa-miR-18a-5p/FDX1 axis suppresses proliferation and drives cuproptosis and oxaliplatin sensitivity of hepatocellular carcinoma. Am J Cancer Res 13:5590-5609 (2023). PubMed: 38058825
- Dai L et al. Systematic analysis based on the cuproptosis-related genes identifies ferredoxin 1 as an immune regulator and therapeutic target for glioblastoma. BMC Cancer 23:1249 (2023). PubMed: 38114959
- Chu B et al. The core genes of cuproptosis assists in discerning prognostic and immunological traits of clear cell renal cell carcinoma. Front Oncol 12:925411 (2022). PubMed: 36212447
- Zhang K et al. A Novel Cuproptosis-Related Prognostic Model and the Hub Gene FDX1 Predict the Prognosis and Correlate with Immune Infiltration in Clear Cell Renal Cell Carcinoma. J Oncol 2022:2124088 (2022). PubMed: 36536785
- Zhang J et al. FDXR regulates TP73 tumor suppressor via IRP2 to modulate aging and tumor suppression. J Pathol N/A:N/A (2020). PubMed: 32304229
- Zhang Y et al. Ferredoxin reductase is critical for p53-dependent tumor suppression via iron regulatory protein 2. Genes Dev N/A:N/A (2017). WB . PubMed: 28747430
- Li J et al. De novo synthesis of steroids and oxysterols in adipocytes. J Biol Chem 289:747-64 (2014). WB . PubMed: 24280213