AB249836

重组Anti-AdSS 2抗体[EPR12331-73] - BSA and Azide free

Anti-AdSS 2 antibody [EPR12331-73] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal AdSS 2 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

ADSS, ADSS2, Adenylosuccinate synthetase isozyme 2, AMPSase 2, AdSS 2, IMP--aspartate ligase 2, L-type adenylosuccinate synthetase

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-AdSS 2 antibody [EPR12331-73] - BSA and Azide free (AB249836)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-AdSS 2 antibody [EPR12331-73] - BSA and Azide free (AB249836)

This data was developed using ab174848, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of 293 cells labeling AdSS 2 using ab174848 at a 1/50 dilution. DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-AdSS 2 antibody [EPR12331-73] - BSA and Azide free (AB249836)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-AdSS 2 antibody [EPR12331-73] - BSA and Azide free (AB249836)

This data was developed using ab174848, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293 cells labeling AdSS 2 using ab174848 at a 1/10 dilution (red), or a rabbit IgG negative control (green).

Supplier Data

Western blot - Anti-AdSS 2 antibody [EPR12331-73] - BSA and Azide free (AB249836)

This data was developed using ab174848, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-AdSS 2 antibody [EPR12331-73] (<a href='/products/primary-antibodies/adss-2-antibody-epr12331-73-ab174848'>ab174848</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HT-1376 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

U87-MG cell lysate at 10 µg

Predicted band size: 50 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-AdSS 2 antibody [EPR12331-73]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12331-73

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab249836 is the carrier-free version of ab174848.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AdSS 2 also known as Adenylate Succinate Synthase 2 is an enzyme that functions in the cellular ATP generation process. It catalyzes an important step in the purine nucleotide cycle specifically the conversion of inosine monophosphate (IMP) to adenylosuccinate. This enzyme has an approximate molecular weight of 55 kDa. AdSS 2 is widely expressed in tissues with high energy demands such as liver muscle and brain where it plays a major role in maintaining cellular energy balance.

Biological function summary

AdSS 2 is important for the synthesis of AMP from IMP acting as a part of the purine biosynthesis pathway. It does not work alone; it associates with other enzymes involved in the nucleotide metabolism complex ensuring efficiency of ATP production. This activity supports various cellular functions that require rapid energy turnover reflecting its necessity in tissues with intense metabolic activity.

Pathways

This enzyme integrates into the broader purine metabolism and energy homeostasis pathways. It connects with the enzyme fumarase in a pathway that regulates the TCA cycle's connection to nucleotide metabolism. Furthermore its interaction with proteins like adenylosuccinate lyase shows its involvement in the cascading steps of purine synthesis helping maintain a balance between energy supply and demand.

AdSS 2 has relevant implications in metabolic conditions like gout and certain types of myopathies. Mutations in this enzyme can disrupt purine metabolism leading to excessive accumulation of uric acid a hallmark of gout. Similarly impaired AdSS 2 function links to muscle disorders where energy metabolism is compromised. Proteins such as adenylosuccinate lyase and fumarase may be co-involved in these metabolic disorders highlighting the interconnected nature of metabolic function and disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.

See full target information ADSS2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma

View all publications

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-AdSS 2抗体[EPR12331-73] - BSA and Azide free