AB174842

重组Anti-AdSS 2抗体[EPR12331-52]

Anti-AdSS 2 antibody [EPR12331-52]

RabMAb
Recombinant
了解详情

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(2 Publications)

Rabbit Recombinant Monoclonal AdSS 2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

查看别名

ADSS, ADSS2, Adenylosuccinate synthetase isozyme 2, AMPSase 2, AdSS 2, IMP--aspartate ligase 2, L-type adenylosuccinate synthetase

2 Images

Supplier Data

Immunoprecipitation - Anti-AdSS 2 antibody [EPR12331-52] (AB174842)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) or 1X PBS (lane 2) (negative control), labeling AdSS 2 immunoprecipitated using ab174842 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-AdSS 2 antibody [EPR12331-52] (ab174842)

Predicted band size: 50 kDa

false

Supplier Data

Western blot - Anti-AdSS 2 antibody [EPR12331-52] (AB174842)

All lanes:

Western blot - Anti-AdSS 2 antibody [EPR12331-52] (ab174842) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HT1376 cell lysate at 10 µg

Lane 3:

HepG2 cell lysates at 10 µg

Predicted band size: 50 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-AdSS 2 antibody [EPR12331-52] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12331-52

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

AdSS 2 also known as Adenylate Succinate Synthase 2 is an enzyme that functions in the cellular ATP generation process. It catalyzes an important step in the purine nucleotide cycle specifically the conversion of inosine monophosphate (IMP) to adenylosuccinate. This enzyme has an approximate molecular weight of 55 kDa. AdSS 2 is widely expressed in tissues with high energy demands such as liver muscle and brain where it plays a major role in maintaining cellular energy balance.

Biological function summary

AdSS 2 is important for the synthesis of AMP from IMP acting as a part of the purine biosynthesis pathway. It does not work alone; it associates with other enzymes involved in the nucleotide metabolism complex ensuring efficiency of ATP production. This activity supports various cellular functions that require rapid energy turnover reflecting its necessity in tissues with intense metabolic activity.

Pathways

This enzyme integrates into the broader purine metabolism and energy homeostasis pathways. It connects with the enzyme fumarase in a pathway that regulates the TCA cycle's connection to nucleotide metabolism. Furthermore its interaction with proteins like adenylosuccinate lyase shows its involvement in the cascading steps of purine synthesis helping maintain a balance between energy supply and demand.

AdSS 2 has relevant implications in metabolic conditions like gout and certain types of myopathies. Mutations in this enzyme can disrupt purine metabolism leading to excessive accumulation of uric acid a hallmark of gout. Similarly impaired AdSS 2 function links to muscle disorders where energy metabolism is compromised. Proteins such as adenylosuccinate lyase and fumarase may be co-involved in these metabolic disorders highlighting the interconnected nature of metabolic function and disease.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.

See full target information ADSS2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 29:1878-1894 PubMed35840668

2022

MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Cai Zhang,Nicholas Skiados,Fareesa Aftab,Cerena Moreno,Luis Silva,Paul Joshua Anthony Corbilla,John M Asara,Aaron N Hata,Frank J Slack

Frontiers in cell and developmental biology 9:635424 PubMed33748123

2021

Autosomal Recessive Rod-Cone Dystrophy Associated With Compound Heterozygous Variants in Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Leming Fu,Ya Li,Shun Yao,Qingge Guo,Ya You,Xianjun Zhu,Bo Lei

View all publications

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重组Anti-AdSS 2抗体[EPR12331-52]