重组Anti-Adipose Triglyceride Lipase (phospho S406)抗体[EPR27159-29] (ab317610)
![Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)](https://www.abcam.cn/ps/products/317/ab317610/Images/ab317610-542215-anti-adipose-triglyceride-lipase-antibody-epr27159-29-western-blot-undifferentiated-3t3-l1-mouse-embryonic-fibroblast-whole-cell-lysate-40-ug-untreated-membrane.jpg "Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27159-29] to Adipose Triglyceride Lipase (phospho S406)
- Suitable for: WB, ICC/IF, IP, Indirect ELISA
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Adipose Triglyceride Lipase (phospho S406)抗体[EPR27159-29]
参阅全部 Adipose Triglyceride Lipase 一抗 -
描述
兔单克隆抗体[EPR27159-29] to Adipose Triglyceride Lipase (phospho S406) -
宿主
Rabbit -
特异性
This antibody recognizes both mouse phospho Ser406 and human phospho Ser404.
-
经测试应用
适用于: WB, ICC/IF, IP, Indirect ELISAmore details
不适用于: Flow Cyt (Intra) or IHC-P -
种属反应性
与反应: Mouse, Human
不与反应: Rat -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
阳性对照
- WB: 3T3-L1 differentiated for 6 days whole cell lysate and Mouse white fat tissue lysate. ICC/IF: 3T3-L1 cells. IP: Differentiated 3T3-L1 for 6 days whole cell lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR27159-29 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317610于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 55 kDa.
|
|
| ICC/IF |
1/50.
|
|
| IP |
1/30.
|
|
| Indirect ELISA |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 55 kDa. |
|
ICC/IF
1/50. |
|
IP
1/30. |
|
Indirect ELISA
Use a concentration of 1 µg/ml. |
靶标
-
功能
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. -
组织特异性
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). -
通路
Glycerolipid metabolism; triacylglycerol degradation. -
疾病相关
Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells. -
序列相似性
Contains 1 patatin domain. -
发展阶段
Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium. -
细胞定位
Lipid droplet. Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 57104 Human
- Entrez Gene: 66853 Mouse
- Omim: 609059 Human
- SwissProt: Q96AD5 Human
- SwissProt: Q8BJ56 Mouse
- Unigene: 654697 Human
- Unigene: 29998 Mouse
-
别名
- 1110001C14Rik antibody
- Adipose triglyceride lipase antibody
- ATGL antibody
see all
图片
-
Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)All lanes : Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610) at 1/1000 dilution
Lane 1 : Undifferentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate (untreated membrane)
Lane 2 : 3T3-L1 differentiated for 6 days whole cell lysate (untreated membrane)
Lane 3 : Undifferentiated 3T3-L1 whole cell lysate (Lambda phosphatase treated membrane)
Lane 4 : 3T3-L1 differentiated for 6 days whole cell lysate (Lambda phosphatase treated membrane)
Lane 5 : Mouse white fat tissue lysate (untreated membrane)
Lane 6 : Mouse white fat tissue lysate (Lambda phosphatase treated membrane)
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)](https://www.abcam.cn/ps/products/317/ab317610/Images/ab317610-1-anti-adipose-triglyceride-lipase-antibody-epr27159-29-immunocytochemistry-3t3-l1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling Adipose Triglyceride Lipase with ab317610 at 1/50 (10.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic and membranous staining in differentiated 3T3-L1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
-
![Immunoprecipitation - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)](https://www.abcam.cn/ps/products/317/ab317610/Images/ab317610-2-anti-adipose-triglyceride-lipase-antibody-epr27159-29-immunoprecipitation-differentiated-3t3-l1.jpg)
Immunoprecipitation - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)Adipose Triglyceride Lipase was immunoprecipitated from 0.35 mg Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate with ab317610 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317610 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 2: ab317610 IP in Differentiated 3T3-L1(mouse embryonic fibroblast) for 6 days whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317610 in differentiated 3T3-L1 for 6 days whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds.
-
![Indirect ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)](https://www.abcam.cn/ps/products/317/ab317610/Images/ab317610-542266-anti-adipose-triglyceride-lipase-antibody-epr27159-29-elisa-mouse.jpg)
Indirect ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)Indirect ELISA analysis of ab317610 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Mouse Adipose Triglyceride Lipase (phospho S406), Mouse Adipose Triglyceride Lipase non-phospho, Mouse Adipose Triglyceride Lipase (mutated S406A), Human Adipose Triglyceride Lipase (phospho S404), Human Adipose Triglyceride Lipase non-phospho.
Antigen concentration: 100ng/ml
-
![Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)](https://www.abcam.cn/ps/products/317/ab317610/Images/ab317610-3-benefitsofrecombinantantibodies.jpg)
Anti-Adipose Triglyceride Lipase (phospho S406) antibody [EPR27159-29] (ab317610)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (0)
ab317610 尚未被引用在任何文献中。