AB135093

Anti-Adipose Triglyceride Lipase (phospho S406) 抗体

Name: Adipose Triglyceride Lipase(磷酸化S406)抗体 (ab135093)| Abcam中文官网
Brand: Abcam Limited
SKU: AB135093
Price: 5598 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-Adipose Triglyceride Lipase (phospho S406) antibody

(1 Review)

(35 Publications)

Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) is a rabbit polyclonal antibody detecting Adipose Triglyceride Lipase in Western Blot, ELISA. Suitable for Mouse.

- Over 20 publications

查看别名

Atgl, Pnpla2, Patatin-like phospholipase domain-containing protein 2, Adipose triglyceride lipase, Calcium-independent phospholipase A2-zeta, Desnutrin, iPLA2-zeta

2 Images

Lab

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (AB135093)

All lanes:

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) at 1 µg/mL

Lane 1:

Mouse Brown Adipose Tissue (BAT) at 20 µg

Lane 2:

Mouse Brown Adipose Tissue (BAT) at 20 µg with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 412) (Phosphorylation S 406)

Lane 3:

Mouse Brown Adipose Tissue (BAT) at 20 µg with Mouse Patatin-like phospholipase domain-containing protein 2 Synth (402 - 413) (No Modifications)

Predicted band size: 55 kDa

Observed band size: 35 kDa,55 kDa,85 kDa

true

Exposure time: 20min

ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (AB135093)

ELISA

Unknown

ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (AB135093)

ab135093 was tested using an Indirect ELISA approach. The wells were coated with peptide (1μg/ml at 100μl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025μg/ml (100μl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1 : 20,000 dilution for 1hr at room temperature.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse

应用

ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Adipose Triglyceride Lipase (phospho S406) antibody (ab135093) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), ELISA in Mouse samples.

What is the molecular weight of Adipose Triglyceride Lipase?
Anti-Adipose Triglyceride Lipase (phospho S406) (ab135093) specifically detects a band for Adipose Triglyceride Lipase (UniProt: Q8BJ56) at a molecular weight of 55kDa.

Trusted by the scientific community
Anti-Adipose Triglyceride Lipase (phospho S406) (ab135093) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Adipose Triglyceride Lipase often referred to as ATGL is an important enzyme in lipid metabolism. Also known as lipase 29 or desnutrin ATGL hydrolyzes triglycerides into diglycerides and free fatty acids the first step in the breakdown of stored fat. The molecular weight of the ATGL protein is approximately 54 kDa. Cells express ATGL abundantly in adipose tissues but it also appears in cardiac and skeletal muscles highlighting its importance in energy management across different tissues.

Biological function summary

ATGL is part of the lipid mobilization process and contributes significantly to energy homeostasis. It acts distinctively as it breaks down phosphotriglycerides and is not part of a larger complex carrying out its function independently. Its activity impacts the balance of lipid storage and release important for maintaining energy levels within cells and across the entire organism.

Pathways

ATGL plays a significant role in the lipid catabolism and energy regulation pathways. ATGL's activity regulates the fat mobilization pathway by interacting closely with the protein Comparative Gene Identification-58 (CGI-58) which activates it. Further ATGL is involved in the peroxisome proliferator-activated receptor (PPAR) pathway highlighting its role in influencing metabolic processes and energy balance.

ATGL dysregulation connects to metabolic disorders such as obesity and non-alcoholic fatty liver disease (NAFLD). Reduced ATGL activity leads to excessive triglyceride accumulation in adipose tissue contributing to the development of obesity. Moreover ATGL deficiencies can link to cardiac issues through its connection with another lipase Hormone-Sensitive Lipase (HSL) affecting lipid content in cardiac tissues and influencing cardiomyopathies. Understanding ATGL's precise function aids in the development of therapeutic strategies for these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed : 15337759, PubMed : 15550674, PubMed : 16150821, PubMed : 16675698, PubMed : 16679289, PubMed : 17074755, PubMed : 17114792, PubMed : 23066022). Exhibits a strong preference for the hydrolysis of long-chain fatty acid esters at the sn-2 position of the glycerol backbone and acts coordinately with LIPE/HLS and DGAT2 within the lipolytic cascade (PubMed : 23066022). Also possesses acylglycerol transacylase and phospholipase A2 activities (By similarity). Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides (By similarity). Regulates adiposome size and may be involved in the degradation of adiposomes (By similarity). Catalyzes the formation of an ester bond between hydroxy fatty acids and fatty acids derived from triglycerides or diglycerides to generate fatty acid esters of hydroxy fatty acids (FAHFAs) in adipocytes (PubMed : 35676490). Acts antagonistically with LDAH in regulation of cellular lipid stores (By similarity). Inhibits LDAH-stimulated lipid droplet fusion (By similarity). May play an important role in energy homeostasis (PubMed : 16675698). May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion (PubMed : 15337759).

See full target information Pnpla2 phospho S406

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文献 (35)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6567 PubMed40670400

2025

Adipose tissue-derived PRXL2A suppresses hepatic lipogenesis in a study with male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyuan Li,Zheng Tian,Xiaoliu Shi,Aijun Long,Yazhuo Wang,Yan Yang,Yaqi Wang,Jingjing Zhang,Yiguo Wang

Nature communications 16:3111 PubMed40169574

2025

Exercise-induced anti-obesity effects in male mice generated by a FOXO1-KLF10 reinforcing loop promoting adipose lipolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Ying Zhu,Min Chen,Wang-Jing Mu,Hong-Yang Luo,Yang Li,Shan Li,Lin-Jing Yan,Ruo-Ying Li,Meng-Ting Yin,Xin Li,Hu-Min Chen,Liang Guo

Journal of lipid research 66:100700 PubMed39566849

2024

DFCP1 is a regulator of starvation-driven ATGL-mediated lipid droplet lipolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria A Ismail,Meg Schuetz,Zak N Baker,Jean A Castillo-Badillo,Teri V Naismith,David J Pagliarini,David J Kast

iScience 27:109468 PubMed38550985

2024

Creatine and low-dose lithium supplementation separately alter energy expenditure, body mass, and adipose metabolism for the promotion of thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

M S Finch,G L Gardner,J L Braun,M S Geromella,J Murphy,K Colonna,R Dhaliwal,A Retta,A Mohammad,J A Stuart,P J LeBlanc,V A Fajardo,B D Roy,R E K MacPherson

The journal of physiological sciences : JPS 74:8 PubMed38331728

2024

The role of GPR81-cAMP-PKA pathway in endurance training-induced intramuscular triglyceride accumulation and mitochondrial content changes in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Li,Xiangdeng Lai,Yihan Ni,Siyu Chen,Yaqian Qu,Zhiqiang Hu,Jingquan Sun

Aging cell 22:e14009 PubMed37960952

2023

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zer Vue,Edgar Garza-Lopez,Kit Neikirk,Prasanna Katti,Larry Vang,Heather Beasley,Jianqiang Shao,Andrea G Marshall,Amber Crabtree,Alexandria C Murphy,Brenita C Jenkins,Praveena Prasad,Chantell Evans,Brittany Taylor,Margaret Mungai,Mason Killion,Dominique Stephens,Trace A Christensen,Jacob Lam,Benjamin Rodriguez,Mark A Phillips,Nastaran Daneshgar,Ho-Jin Koh,Alice Koh,Jamaine Davis,Nina Devine,Mohammad Saleem,Estevão Scudese,Kenneth Ryan Arnold,Valeria Vanessa Chavarin,Ryan Daniel Robinson,Moumita Chakraborty,Jennifer A Gaddy,Mariya T Sweetwyne,Genesis Wilson,Elma Zaganjor,James Kezos,Cristiana Dondi,Anilkumar K Reddy,Brian Glancy,Annet Kirabo,Anita M Quintana,Dao-Fu Dai,Karen Ocorr,Sandra A Murray,Steven M Damo,Vernat Exil,Blake Riggs,Bret C Mobley,Jose A Gomez,Melanie R McReynolds,Antentor Hinton

Obesity (Silver Spring, Md.) 31:1859-1870 PubMed37254272

2023

An Artemisia scoparia extract attenuates glucocorticoid-induced lipolysis in adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Innocence Harvey,Jacqueline M Stephens

The Journal of biological chemistry 299:104635 PubMed36963490

2023

Inhibition of nucleotide biosynthesis disrupts lipid accumulation and adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Abhijit B Shinde,Elizabeth R Nunn,Genesis A Wilson,Mathew T Chvasta,Julia A Pinette,Jacob W Myers,Sun H Peck,Jessica B Spinelli,Elma Zaganjor

Molecular biology reports 50:2591-2601 PubMed36626064

2023

Intramuscular mitochondrial and lipid metabolic changes of rats after regular high-intensity interval training (HIIT) of different training periods.

Applications

Unspecified application

Species

Unspecified reactive species

Ruonan Shangguan,Zhiqiang Hu,Yuzhen Luo,Min Chen,Xiangdeng Lai,Jingquan Sun,Siyu Chen

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:541-555 PubMed36606556

2023

Sclerostin Influences Exercise-Induced Adaptations in Body Composition and White Adipose Tissue Morphology in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nigel Kurgan,Joshua Stoikos,Bradley J Baranowski,Jenalyn Yumol,Roopan Dhaliwal,Jake B Sweezey-Munroe,Val A Fajardo,William Gittings,Rebecca E K Macpherson,Panagiota Klentrou

View all publications

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Anti-Adipose Triglyceride Lipase (phospho S406) 抗体

Anti-Adipose Triglyceride Lipase (phospho S406) antibody

Western blot - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (AB135093)

ELISA - Anti-Adipose Triglyceride Lipase (phospho S406) antibody (AB135093)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性