重组Anti-Adipose Triglyceride Lipase抗体[EPR19650-246] - BSA and Azide free (Capture) (ab272317)
![Sandwich ELISA - Anti-Adipose Triglyceride Lipase antibody [EPR19650-246] - BSA and Azide free (Capture) (ab272317)](https://www.abcam.cn/ps/products/272/ab272317/Images/ab272317-2-human-atgl-antibody-pair-sandwich-elisa-standard-curve-2.jpg "Sandwich ELISA - Anti-Adipose Triglyceride Lipase antibody [EPR19650-246] - BSA and Azide free (Capture) (ab272317)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19650-246] to Adipose Triglyceride Lipase - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
概述
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产品名称
Anti-Adipose Triglyceride Lipase抗体[EPR19650-246] - BSA and Azide free (Capture)
参阅全部 Adipose Triglyceride Lipase 一抗 -
描述
兔单克隆抗体[EPR19650-246] to Adipose Triglyceride Lipase - BSA and Azide free (Capture) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab272317 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ATGL. The recommended pair for sandwich ELISA is:
Capture: ab272317, Human ATGL Capture Antibody (unconjugated)
Detector: ab272318, Human ATGL Detector Antibody (unconjugated)
The reference range value is 218.75 - 14000 pg/ml.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19650-246 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272317于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
|
| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
靶标
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功能
Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. -
组织特异性
Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). -
通路
Glycerolipid metabolism; triacylglycerol degradation. -
疾病相关
Note=Genetic variations in PNPLA2 may be associated with risk of diabetes mellitus type 2.
Defects in PNPLA2 are the cause of neutral lipid storage disease with myopathy (NLSDM) [MIM:610717]; also known as neutral lipid storage disease without ichthyosis. NSLDM is a neutral lipid storage disorder (NLSD) with myopathy but without ichthyosis. NLSDs are characterized by the presence of triglyceride-containing cytoplasmic droplets in leukocytes and in other tissues, including bone marrow, skin, and muscle. Individuals with NLSDM did not show obesity, in spite of a defect in triglyceride degradation in fibroblasts and in marked triglyceride storage in liver, muscles, and other visceral cells. -
序列相似性
Contains 1 patatin domain. -
发展阶段
Induced during differentiation of primary preadipocytes to adipocytes. Expression increased from fetal to adult in retinal pigment epithelium. -
细胞定位
Lipid droplet. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 57104 Human
- Omim: 609059 Human
- SwissProt: Q96AD5 Human
- Unigene: 654697 Human
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别名
- 1110001C14Rik antibody
- Adipose triglyceride lipase antibody
- ATGL antibody
see all
图片
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Sandwich ELISA - Anti-Adipose Triglyceride Lipase antibody [EPR19650-246] - BSA and Azide free (Capture) (ab272317)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270890).
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![Anti-Adipose Triglyceride Lipase antibody [EPR19650-246] - BSA and Azide free (Capture) (ab272317)](https://www.abcam.cn/ps/products/272/ab272317/Images/ab272317-1-benefits-of-recombinant-antibodies.png)
Anti-Adipose Triglyceride Lipase antibody [EPR19650-246] - BSA and Azide free (Capture) (ab272317)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab272317 尚未被引用在任何文献中。
