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Anti-ADIPOR1抗体[mAbcam50675] (ab50675)

Submit a review Q&A (1)References (3)
Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
  • Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)

Key features and details

  • Mouse monoclonal [mAbcam50675] to ADIPOR1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgM

概述

  • 产品名称

    Anti-ADIPOR1抗体[mAbcam50675]
    参阅全部 ADIPOR1 一抗

  • 描述

    小鼠单克隆抗体[mAbcam50675] to ADIPOR1

  • 宿主

    Mouse

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Zebrafish

  • 免疫原

    Synthetic peptide corresponding to Human ADIPOR1 aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab59184)

  • 阳性对照

    • This antibody gave a positive signal in Human Skeletal Muscle Lysate.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...

  • 纯度

    Concentrated Culture Supernatant

  • 纯化说明

    Tissue culture supernatant was concentrated and then buffer exchanged.

  • 克隆

    单克隆

  • 克隆编号

    mAbcam50675

  • 骨髓瘤

    Sp2/0-Ag14

  • 同种型

    IgM

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab50675于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
说明
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).

靶标

  • 功能

    Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some high-affinity receptor for globular adiponectin but low-affinity receptor for full-length adiponectin.

  • 组织特异性

    Widely expressed. Highly expressed in skeletal muscle. Expressed at intermediate level in brain, heart, spleen, kidney, liver, placenta, lung and peripheral blood leukocytes. Weakly expressed in colon, thymus and small intestine.

  • 序列相似性

    Belongs to the ADIPOR family.

  • 结构域

    The N-terminus is known to be cytoplasmic while the C-terminus is known to be extracellular.

  • 细胞定位

    Membrane. Localized to the cell membrane and intracellular organelles.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ACDCR1 antibody
    • ADIPO R1 antibody
    • Adiponectin receptor protein 1 antibody
    • ADIPOR 1 antibody
    • Adipor1 antibody
    • ADR1_HUMAN antibody
    • CGI 45 antibody
    • CGI 45 protein antibody
    • CGI-45 antibody
    • CGI45 antibody
    • CGI45 protein antibody
    • FLJ25385 antibody
    • FLJ42464 antibody
    • PAQR1 antibody
    • Progestin and adipoQ receptor family member I antibody
    • TESBP1A antibody
    see all

图片

  • Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Anti-ADIPOR1 antibody [mAbcam50675] (ab50675) at 10 µg/ml + Human skeletal muscle tissue lysate - total protein (ab29330) at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 28 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Western blot - Anti-ADIPOR1 antibody [mAbcam50675] (ab50675)
    Anti-ADIPOR1 antibody [mAbcam50675] (ab50675) at 10 µg/ml + Human Skeletal Muscle tissue lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L at 1/5000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 12 minutes


    Blocking buffer: 3% Milk
    Gel type: MOPS

文献 (3)

发表研究结果有使用 ab50675？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab50675 被引用在 3 文献中.

  • Wang X  et al. Schisandrin A from Schisandra chinensis Attenuates Ferroptosis and NLRP3 Inflammasome-Mediated Pyroptosis in Diabetic Nephropathy through Mitochondrial Damage by AdipoR1 Ubiquitination. Oxid Med Cell Longev 2022:5411462 (2022). PubMed: 35996380
  • Wang H  et al. 5-Hydroxytryptophan Suppresses the Abdominal Fat Deposit and Is Beneficial to the Intestinal Immune Function in Broilers. Front Physiol 11:655 (2020). PubMed: 32595527
  • Diot M  et al. Expression of adiponectin, chemerin and visfatin in plasma and different tissues during a laying season in turkeys. Reprod Biol Endocrinol 13:81 (2015). WB ; Turkey . PubMed: 26228641

客户评价及客户问答

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Question

I am using ab50675 in WB and cannot detect a signal on human cell lysate colon cancer cells. The protocol advice I received to use more antibody did not help.

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ******. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

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