重组Anti-Adiponectin抗体[EPR3218] (ab133347)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3218] to Adiponectin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Adiponectin抗体[EPR3218]
参阅全部 Adiponectin 一抗 -
描述
兔单克隆抗体[EPR3218] to Adiponectin -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fat, placenta and plasma lysates. HUH7, Human adipose and plasma lysate. Mouse white adipose and plasma lysate. Rat white adipose and plasma lysate. Rat-1 whole cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3218 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133347于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
靶标
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功能
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. -
组织特异性
Synthesized exclusively by adipocytes and secreted into plasma. -
疾病相关
Defects in ADIPOQ are the cause of adiponectin deficiency (ADPND) [MIM:612556]. ADPND results in very low concentrations of plasma adiponectin.
Genetic variations in ADIPOQ are associated with non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance. -
序列相似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
结构域
The C1q domain is commonly called the globular domain. -
翻译后修饰
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting.
HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes.
O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 9370 Human
- Entrez Gene: 11450 Mouse
- Entrez Gene: 246253 Rat
- Omim: 605441 Human
- SwissProt: Q15848 Human
- SwissProt: Q60994 Mouse
- Unigene: 80485 Human
- Unigene: 3969 Mouse
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别名
- 30 kDa adipocyte complement related protein antibody
- 30 kDa adipocyte complement-related protein antibody
- ACDC antibody
see all
图片
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All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)
Lane 1 : Mouse white adipose lysate
Lane 2 : Mouse plasma lysate
Lane 3 : Rat-1 (Rat embryonic fibroblast) whole cell lysate
Lane 4 : Rat white adipose lysate
Lane 5 : Rat plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).
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All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution (Purified)
Lane 1 : Huh7 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : Human adipose lysate
Lane 3 : Human plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?The expression profile and molecular weight observed are consistent with what have been described in the literatures (PMID: 15855314, 23967179).
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All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347) at 1/1000 dilution
Lane 1 : Human fat lysate
Lane 2 : Human placenta lysate
Lane 3 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
All lanes : Anti-Adiponectin antibody [EPR3218] (ab133347)
Lane 1 : Human plasma lysate treated with Beta-ME and heat
Lane 2 : Human plasma lysate treated with heat only
Lane 3 : Human plasma lysate treated with Beta-ME only
Lane 4 : Human plasma lysate untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab133347 被引用在 4 文献中.
- Chen Y et al. Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis. Gut Microbes 15:2211501 (2023). PubMed: 37203220
- Beč A et al. Design, synthesis, biological evaluation and QSAR analysis of novel N-substituted benzimidazole derived carboxamides. J Enzyme Inhib Med Chem 37:1327-1339 (2022). PubMed: 35514167
- Roškarić P et al. Low Dietary n6/n3 Ratio Attenuates Changes in the NRF 2 Gene Expression, Lipid Peroxidation, and Inflammatory Markers Induced by Fructose Overconsumption in the Rat Abdominal Adipose Tissue. Antioxidants (Basel) 10:N/A (2021). PubMed: 34943108
- Peng YJ et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). PubMed: 29540173