Anti-Adenovirus Type 5抗体(ab6982)
Key features and details
- Rabbit polyclonal to Adenovirus Type 5
- Suitable for: Neutralising, ELISA, WB, ICC/IF
- Reacts with: Human adenovirus 5
- Isotype: IgG
概述
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产品名称
Anti-Adenovirus Type 5抗体 -
描述
兔多克隆抗体to Adenovirus Type 5 -
宿主
Rabbit -
经测试应用
适用于: Neutralising, ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Human adenovirus 5 -
免疫原
Tissue, cells or virus corresponding to Adenovirus Type 5. Purified Adenovirus type 5
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Neutralising |
Use at an assay dependent concentration.
This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137 |
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| ELISA | (1) |
Use at an assay dependent concentration.
Recognizes Ad5 immobilized on microtiter plates. |
| WB | (5) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Neutralising
Use at an assay dependent concentration. This antibody will neutralize Ad5 infection in a bioassay (1/25,000 dilution for 50 % inactivation of Ad5at 1000 particles/ml) - PMID: 14605137 |
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ELISA
Use at an assay dependent concentration. Recognizes Ad5 immobilized on microtiter plates. |
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
靶标
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相关性
Adenoviruses are DNA viruses that cause upper respiratory tract infections, conjunctivitis, and other infections in humans. Adenoviruses were discovered in 1953. About 47 different types have been identified since then, and about half of them are believed to cause human diseases. Infants and children are most commonly affected by adenoviruses. Adenovirus infections can occur throughout the year, but seem to be most common from fall to spring. Adenoviruses are responsible for 3-5% of acute respiratory infections in children and 2% of respiratory illnesses in adults. -
别名
- Adenovirus 5 antibody
- AdV 5 antibody
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.ab6982 staining Adenovirus type 5 in human HeLa cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton and then blocked using 0.1% BSA for 16 hours at 4°C. Samples were then incubated with primary antibody at 1/800 for 1 hour at 37°C. The secondary antibody used was a donkey anti-rabbit IgG conjugated to Alexa Fluor® 546 (red) used at a 1/1000 dilution.
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Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte).
Bottom blot (B): As above, except ab6982 omitted.
Top blot (A): ab6982 at a 1/4000 dilution staining Adenovirus type 5 capsid proteins by Western blot (ECL). Secondary = goat anti-rabbit (HRP conjuagte). Bottom blot (B): As above, except ab6982 omitted. -
Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous AbreviewAll lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
All lanes : human hepatocytes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit secondary polyclonal antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocked with 5% milk
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Western blot - Anti-Adenovirus Type 5 antibody (ab6982)Image courtesy of an anonymous Abreview.All lanes : Anti-Adenovirus Type 5 antibody (ab6982) at 1/10000 dilution
Lane 1 : Whole cell lysate prepared from U2OS cells.
Lane 2 : Whole cell lysate prepared from U2OS cells infected with Adenovirus 5.
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Goat anti-rabbit conjugated to IRDye 700DX
Blocking step was performed using 5% milk for 1 hour at 23°C.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (79)
ab6982 被引用在 79 文献中.
- Sangare K et al. E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses. Vaccines (Basel) 10:N/A (2022). PubMed: 35214753
- Su W et al. Self-attenuating adenovirus enables production of recombinant adeno-associated virus for high manufacturing yield without contamination. Nat Commun 13:1182 (2022). PubMed: 35256603
- Bergquist H et al. Structural Insights into Human Adenovirus Type 4 Virus-Associated RNA I. Int J Mol Sci 23:N/A (2022). PubMed: 35328524
- Rappaport AR et al. Low-dose self-amplifying mRNA COVID-19 vaccine drives strong protective immunity in non-human primates against SARS-CoV-2 infection. Nat Commun 13:3289 (2022). PubMed: 35672369
- Araki H et al. Oncolytic virus-mediated p53 overexpression promotes immunogenic cell death and efficacy of PD-1 blockade in pancreatic cancer. Mol Ther Oncolytics 27:3-13 (2022). PubMed: 36212775