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重组RabMAb

重组Anti-Adenosine Receptor A2a抗体[EPR23731-50] - BSA and Azide free (ab273879)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
  • Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23731-50] to Adenosine Receptor A2a - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-Adenosine Receptor A2a抗体[EPR23731-50] - BSA and Azide free
    参阅全部 Adenosine Receptor A2a 一抗

  • 描述

    兔单克隆抗体[EPR23731-50] to Adenosine Receptor A2a - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Expression levels of the target protein vary with sample type and optimisation may be required.  In our hands we are only able to detect ADORA2A in relevant neurological samples and do not recommend this product for use in other samples.

  • 经测试应用

    适用于: IHC-Pmore details
    不适用于: IP or WB

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human cerebrum tissue; Mouse cerebrum tissue; Rat cerebrum tissue.

  • 常规说明

    ab273879 is the carrier-free version of ab260032.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273879于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP or WB.

靶标

  • 功能

    Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family.

  • 结构域

    The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.

  • 翻译后修饰

    Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • A2AAR antibody
    • A2aR antibody
    • AA2AR_HUMAN antibody
    • ADENO antibody
    • Adenosine A2 receptor antibody
    • Adenosine A2a receptor antibody
    • Adenosine receptor A2a antibody
    • Adenosine receptor subtype A2a antibody
    • ADORA 2 antibody
    • ADORA2 antibody
    • ADORA2A antibody
    • hA2aR antibody
    • RDC 8 antibody
    • RDC8 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded human cerebrum (striatum) tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum is observed. The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilutionfollowed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the striatum of the mouse cerebrum (PMID: 21858817). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Adenosine receptor A2a with abab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 21858817, PMID: 29022877). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human diffuse large B-cell lymphoma tissue .

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human hepatocellular carcinoma tissue.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human spleen tissue.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Immunohistochemical analysis of paraffin-embedded human thymoma tissue labeling Adenosine receptor A2a with ab260032 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab260032 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Negative control: No staining observed on human thymoma tissue.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260032).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Tissue Microarrays stained for " Anti-Adenosine Receptor A2a antibody [EPR23731-50]” using " ab260032" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

    Tissue Microarrays stained for " Anti-Adenosine Receptor A2a antibody [EPR23731-50]” using " ab260032" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab260032 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)
    Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free (ab273879)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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