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AB3461

Anti-Adenosine Receptor A2a抗体

Anti-Adenosine Receptor A2a antibody

4

(2 Reviews)

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(43 Publications)

Anti-Adenosine Receptor A2a antibody (ab3461) is a rabbit polyclonal antibody detecting Adenosine Receptor A2a in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 30 publications
- Trusted since 2003

查看别名

RDC8, Adenosine receptor A2a

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB3461)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB3461)

ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (AB3461)
  • ICC

Lab

Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (AB3461)

ICC/IF image of ab3461 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3461, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB3461)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB3461)

ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (AB3461)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Adenosine Receptor A2a antibody (AB3461)

Immunocytochemistry/Immunofluorescence analysis of Adenosine Receptor A2a (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3461 in 3% BSA-PBS at a dilution of 1 : 20 overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB3461)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB3461)

ab3461 labelling Adenosine Receptor A2a in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control (left) by Immunohistochemisty (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB, ICC, IHC-P

applications

免疫原

Synthetic Peptide within Dog ADORA2A aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P11617

特异性

Detects adenosine receptor A2a. This antibody does not detect other AR subtypes.

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替代产品

Primary Antibodies

AB260032

Anti-Adenosine Receptor A2a antibody [EPR23731-50]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [EPR23731-50] (AB260032)

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Primary Antibodies

AB97582

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Western blot - Anti-Adenosine Receptor A2a antibody (AB97582)

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Primary Antibodies

AB101678

Anti-Adenosine Receptor A2a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody (AB101678)

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Primary Antibodies

AB79714

Anti-Adenosine Receptor A2a antibody [7F6-G5-A2]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine Receptor A2a antibody [7F6-G5-A2] (AB79714)

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搭配使用产品

Primary Antibodies

AB229671

Anti-Adenosine A2b Receptor/ADORA2B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A2b Receptor/ADORA2B antibody (AB229671)

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Primary Antibodies

AB82477

Anti-Adenosine A1 Receptor antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A1 Receptor antibody (AB82477)

  • 3
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Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
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Primary Antibodies

AB197350

Anti-Adenosine A3 Receptor/A3AR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Adenosine A3 Receptor/A3AR antibody (AB197350)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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产品详情

What is this antibody validated in?
Anti-Adenosine Receptor A2a antibody (ab3461) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

Trusted by the scientific community
Anti-Adenosine Receptor A2a (ab3461) was first used in a scientific publication in 2003 and has been cited over 30 times in peer-reviewed journals.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Adenosine Receptor A2A also known as A2AR is a G protein-coupled receptor with a molecular mass of approximately 45 kDa. This receptor is widely expressed in various body tissues including the brain heart spleen and immune cells. A2A receptors play a mechanical role in mediating physiological responses to adenosine a purine nucleoside that acts as a signaling molecule in the body. Through binding to adenosine A2A receptors activate intracellular pathways that modulate cellular responses.
Biological function summary

The A2A adenosine receptor functions as an important regulator of neurotransmission and immune response. It does not form part of a larger protein complex but rather independently influences processes such as vasodilation heart rate regulation and cytokine release. The A2A receptor contributes to the modulation of dopamine and glutamate signaling in the central nervous system playing a significant role in maintaining neural balance. Its activity in immune cells helps in controlling inflammation and immune cell proliferation.

Pathways

The A2A adenosine receptor engages in critical signaling cascades particularly the cAMP-PKA pathway and the MAPK pathway. Through the cAMP-PKA pathway A2A R influences various cellular processes by increasing cyclic AMP levels which activate protein kinase A. The receptor is also involved in the MAPK pathway impacting cellular growth and differentiation. It interacts with proteins such as Gs alpha subunit which couples to the receptor to promote downstream signaling events.

The A2A receptor has connections to conditions like Parkinson's disease and ischemia-reperfusion injury. In Parkinson's disease the activity of A2A receptors affects dopaminergic signaling where it interacts with proteins such as dopamine D2 receptors suggesting potential for therapeutic targeting. During ischemia-reperfusion injury the receptor's modulation of immune responses and vasodilation can influence tissue damage and repair mechanisms linking it with proteins involved in inflammatory pathways.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Receptor for adenosine (PubMed : 2125216). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase (PubMed : 2125216).
See full target information ADORA2A
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文献 (43)

Recent publications for all applications. Explore the full list and refine your search

Molecules (Basel, Switzerland) 30: PubMed40733187

2025

The Role of Adenosine A1 and A2a Receptors in Cerebral Blood Vessel Reactivity of Sprague Dawley Rats Exposed to Hyperbaric Oxygenation.

Applications

Unspecified application

Species

Unspecified reactive species

Vedran Đambić,Zrinka Mihaljević,Ines Drenjančević,Ivana Jukić,Petar Šušnjara,Aleksandar Kibel

International journal of biological sciences 20:4532-4550 PubMed39247808

2024

The Essential Role of Angiogenesis in Adenosine 2A Receptor Deficiency-mediated Impairment of Wound Healing Involving c-Ski via the ERK/CREB Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Peng,Renping Xiong,Bo Wang,Xing Chen,Yalei Ning,Yan Zhao,Nan Yang,Jing Zhang,Changhong Li,Yuanguo Zhou,Ping Li

Frontiers in microbiology 15:1355225 PubMed38572243

2024

Symbiotic combination of and inosine alleviates alcohol-induced liver injury by modulating gut dysbiosis and immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wei,Yizhi Pan,Yu Guo,Yin Zhu,Haoran Jin,Yingying Gu,Chuanshuang Li,Yaqin Wang,Jingjing Lin,Yongping Chen,Chunhai Ke,Lanman Xu

Toxics 11: PubMed38133400

2023

Effects of Cyfluthrin Exposure on Neurobehaviour, Hippocampal Tissue and Synaptic Plasticity in Wistar Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxin Xie,Ji Zhao,Xiaoyu Li,Jian Sun,Huifang Yang

Purinergic signalling 20:371-384 PubMed37938538

2023

Modafinil exerts anti-inflammatory and anti-fibrotic effects by upregulating adenosine A and A receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Li,Ji Aee Kim,Seong-Eun Jo,Huisu Lee,Kwan-Chang Kim,Shinkyu Choi,Suk Hyo Suh

Biomedicines 11: PubMed37509688

2023

A2AR Expression and Immunosuppressive Environment Independent of and Mutations in Pseudomyxoma Peritonei.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeki Kusamura,Adele Busico,Elena Conca,Iolanda Capone,Luca Agnelli,Daniele Lorenzini,Silvia Brich,Marta Angelini,Chiara Costanza Volpi,Desirè Viola Trupia,Vincenzo Lagano,Tommaso Torelli,Annunziata Gloghini,Dario Baratti,Marcello Guaglio,Massimo Milione,Marcello Deraco,Federica Perrone

Acta pharmaceutica Sinica. B 13:2778-2794 PubMed37425054

2023

Bone-derived MSCs encapsulated in alginate hydrogel prevent collagen-induced arthritis in mice through the activation of adenosine A receptors in tolerogenic dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gaona Shi,Yu Zhou,Wenshuai Liu,Chengjuan Chen,Yazi Wei,Xinlong Yan,Lei Wu,Weiwei Wang,Lan Sun,Tiantai Zhang

Animal nutrition (Zhongguo xu mu shou yi xue hui) 13:282-288 PubMed37168450

2023

Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Zifang Wu,Jiawei Nie,Deyuan Wu,Shuangbo Huang,Jianzhao Chen,Huajin Liang,Xiangyu Hao,Li Feng,Hefeng Luo,Chengquan Tan

Frontiers in cellular neuroscience 15:783478 PubMed35002628

2021

Cordycepin Ameliorates Synaptic Dysfunction and Dendrite Morphology Damage of Hippocampal CA1 A1R in Cerebral Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Hui Chen,Yuan-Yuan Han,Ying-Jie Shang,Si-Yi Zhuang,Jun-Ni Huang,Bao-Yan Wu,Chu-Hua Li

Science (New York, N.Y.) 374:eabh2858 PubMed34648330

2021

Astrocyte Ca-evoked ATP release regulates myelinated axon excitability and conduction speed.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan Lezmy,I Lorena Arancibia-Cárcamo,Tania Quintela-López,Diane L Sherman,Peter J Brophy,David Attwell
View all publications
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