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Anti-ADAT2抗体(ab122280)

Reviews (2) Submit a question References (1)
Immunocytochemistry - Anti-ADAT2 antibody (ab122280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)

Key features and details

  • Rabbit polyclonal to ADAT2
  • Suitable for: ICC, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-ADAT2抗体

  • 描述

    兔多克隆抗体to ADAT2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    antigen sequence: PKPAASGACS VSAEETEKWM EEAMHMAKEA LENTEVPVGC LMVYNNEVVG KGRNEVNQTK NATRHAEMVA IDQVLDWCRQ SGKSPSEVFE, corresponding to amino acids 7-96 of Human ADAT2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • IHC-P: Human stomach, skin, kidney, and cerebellum tissues; ICC: Human cell line HeLa.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab122280于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
ICC
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Probably participates in deamination of adenosine-34 to inosine in many tRNAs.

  • 序列相似性

    Belongs to the cytidine and deoxycytidylate deaminase family. ADAT2 subfamily.
  • Information by UniProt

  • 数据库链接

    • 别名

      • adat2 antibody
      • ADAT2_HUMAN antibody
      • Adenosine deaminase tRNA specific 2 antibody
      • Adenosine deaminase tRNA specific 2 TAD2 homolog antibody
      • DEADC1 antibody
      • Deaminase domain containing 1 antibody
      • Deaminase domain containing protein 1 antibody
      • Deaminase domain-containing protein 1 antibody
      • dJ20N2 antibody
      • dJ20N2.1 antibody
      • TAD2 antibody
      • tRNA specific adenosine 34 deaminase subunit ADAT2 antibody
      • tRNA specific adenosine deaminase 2 antibody
      • tRNA specific adenosine deaminase 2 homolog antibody
      • tRNA-specific adenosine deaminase 2 antibody
      • tRNA-specific adenosine-34 deaminase subunit ADAT2 antibody
      see all

    图片

    • Immunocytochemistry - Anti-ADAT2 antibody (ab122280)
      Immunocytochemistry - Anti-ADAT2 antibody (ab122280)

      Immunocytochemistry/immunofluorescence analysis of human cell line HeLa labelling with ab122280 at 2 µg/mL shows localization to cytosol & the Golgi apparatus.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAT2 antibody (ab122280)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling ADAT2 with ab122280 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    文献 (1)

    发表研究结果有使用 ab122280？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab122280 被引用在 1 文献中.

    • Johnson LM  et al. Short-term heritable variation overwhelms 200 generations of mutational variance for metabolic traits in Caenorhabditis elegans. Evolution 74:2451-2464 (2020). PubMed: 32989734

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-ADAT2 antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK, Hela, LOVO)
    Gel Running Conditions
    Reduced Denaturing (4-12% Nupage Bis-Tris)
    Loading amount
    15 µg
    Specification
    HEK, Hela, LOVO
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Josef Saltz

    Verified customer

    提交于 Feb 22 2020

    Western blot abreview for Anti-ADAT2 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Caenorhabditis elegans Tissue lysate - whole (whole organism)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    40 µg
    Treatment
    70c for 10 minutes equal parts sample and 2x Laemmli buffer
    Specification
    whole organism
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 29 2018

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