Key features and details
- Rabbit polyclonal to ADAMTS7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
参阅全部 ADAMTS7 一抗
特异性This antibody is specific for the prpetide domain after the second furin cleavage site of the full lenght ADAMTS7 and of the short form ADAMTS7A.
经测试应用适用于: WBmore details
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
The Abpromise guarantee
功能Metalloprotease that may play a role in the degradation of COMP.
组织特异性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.
序列相似性Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 PLAC domain.
Contains 8 TSP type-1 domains.
结构域The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻译后修饰N-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed.
细胞定位Secreted, extracellular space, extracellular matrix. Also found associated with the external cell surface.
- Information by UniProt
- A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
All lanes : Anti-ADAMTS7 antibody (ab45044) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 35 µl
Lane 2 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 30 µl
Lane 3 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 25 µl
Predicted band size: 181 kDa
ab45044 被引用在 3 文献中.
- Jana S et al. Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts. J Am Heart Assoc 7:e010427 (2018). PubMed: 30371322
- Pu X et al. ADAMTS7 cleavage and vascular smooth muscle cell migration is affected by a coronary-artery-disease-associated variant. Am J Hum Genet 92:366-74 (2013). PubMed: 23415669
- Meliopoulos VA et al. MicroRNA regulation of human protease genes essential for influenza virus replication. PLoS One 7:e37169 (2012). WB ; Human . PubMed: 22606348