Anti-ADAMTS13抗体(ab28274)
Key features and details
- Rabbit polyclonal to ADAMTS13
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-ADAMTS13抗体
参阅全部 ADAMTS13 一抗 -
描述
兔多克隆抗体to ADAMTS13 -
宿主
Rabbit -
特异性
ab28274 recognises the metalloproteinase domain of ADAMTS13. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human ADAMTS13.
(Peptide available asab41249) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28274于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Glycosylation and the abundance of cysteine residues gives ADAMTS 13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands at 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa). 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Glycosylation and the abundance of cysteine residues gives ADAMTS 13 an apparent molecular weight of 190 kDa on reduced SDS PAGE gels. Several bands at 110-190 kDa are observed on Western blots, possibly indicating differentially processed ADAMTS 13. Dilution optimised using Chromogenic detection. |
靶标
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功能
Cleaves the vWF multimers in plasma into smaller forms. -
组织特异性
Plasma. Expressed primarily in liver. -
疾病相关
Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. -
序列相似性
Contains 2 CUB domains.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 8 TSP type-1 domains. -
结构域
The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction. -
翻译后修饰
May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 11093 Human
- Entrez Gene: 279028 Mouse
- Entrez Gene: 362091 Rat
- Omim: 604134 Human
- SwissProt: Q76LX8 Human
- SwissProt: Q769J6 Mouse
- Unigene: 131433 Human
- Unigene: 330084 Mouse
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别名
- A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
see all
图片
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Western blot - Anti-ADAMTS13 antibody (ab28274)All lanes : Anti-ADAMTS13 antibody (ab28274) at 1 µg/ml
Lane 1 : Recombinant Human ATS-13 at 0.08 µg
Lane 2 : Recombinant Human ATS-13 at 0.04 µg
Lane 3 : Recombinant Human ATS-13 at 0.02 µg
Predicted band size: 154 kDa
Glycosylation and the abundance of cysteine residues gives ADAMTS-13 an apparent molecular weight of about 190 kDa on reduced SDS PAGE gels.
数据表及文件
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Datasheet download
文献 (4)
ab28274 被引用在 4 文献中.
- Abu El-Asrar AM et al. Differential Expression and Localization of ADAMTS Proteinases in Proliferative Diabetic Retinopathy. Molecules 27:N/A (2022). PubMed: 36144730
- Garland KS et al. Removal of the C-Terminal Domains of ADAMTS13 by Activated Coagulation Factor XI induces Platelet Adhesion on Endothelial Cells under Flow Conditions. Front Med (Lausanne) 4:232 (2017). WB . PubMed: 29326937
- Liu L et al. Changes in von Willebrand factor and ADAMTS-13 in patients following arthroplasty. Mol Med Rep 11:3015-20 (2015). PubMed: 25482054
- Tauchi R et al. ADAMTS-13 is produced by glial cells and upregulated after spinal cord injury. Neurosci Lett 517:1-6 (2012). PubMed: 22425718
