Anti-ADAMTS1抗体(ab39194)
Key features and details
- Rabbit polyclonal to ADAMTS1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ADAMTS1抗体
参阅全部 ADAMTS1 一抗 -
描述
兔多克隆抗体to ADAMTS1 -
宿主
Rabbit -
特异性
Ab39194 recognizes the zymogen of ADAMTS1, and the furin cleaved ADAMTS1, but not the shed form. -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human ADAMTS1 (C terminal). This antibody recognizes the last 28 amino acids of the protein.
Database link: Q9UHI8 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39194于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. -
序列相似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains. -
结构域
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 9510 Human
- Entrez Gene: 11504 Mouse
- Entrez Gene: 79252 Rat
- Omim: 605174 Human
- SwissProt: Q9UHI8 Human
- SwissProt: P97857 Mouse
- SwissProt: Q9WUQ1 Rat
- Unigene: 643357 Human
see all -
别名
- A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
- ADAM-TS 1 antibody
- ADAM-TS1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-ADAMTS1 antibody (ab39194)ICC/IF image of ab39194 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39194, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab39194 被引用在 11 文献中.
- Abu El-Asrar AM et al. Differential Expression and Localization of ADAMTS Proteinases in Proliferative Diabetic Retinopathy. Molecules 27:N/A (2022). PubMed: 36144730
- Park M et al. ADAMTS-1: a novel target gene of an estrogen-induced transcription factor, EGR1, critical for embryo implantation in the mouse uterus. Cell Biosci 11:155 (2021). PubMed: 34348778
- Kazakevych J et al. Smarcad1 mediates microbiota-induced inflammation in mouse and coordinates gene expression in the intestinal epithelium. Genome Biol 21:64 (2020). PubMed: 32160911
- Dosh RH et al. Long-term in vitro 3D hydrogel co-culture model of inflammatory bowel disease. Sci Rep 9:1812 (2019). PubMed: 30755679
- Dosh RH et al. Interleukin 1 is a key driver of inflammatory bowel disease-demonstration in a murine IL-1Ra knockout model. Oncotarget 10:3559-3575 (2019). PubMed: 31191826