AB218242

重组Anti-ADAM9抗体[EPR21924-164]

Anti-ADAM9 antibody [EPR21924-164]

RabMAb
Recombinant
KO Validated
了解详情

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Recombinant Monoclonal ADAM9 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications.

查看别名

KIAA0021, MCMP, MDC9, MLTNG, ADAM9, Disintegrin and metalloproteinase domain-containing protein 9, ADAM 9, Cellular disintegrin-related protein, Meltrin-gamma, Metalloprotease/disintegrin/cysteine-rich protein 9, Myeloma cell metalloproteinase

3 Images

Supplier Data

Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (AB218242)

ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10μg (input).
Lane 2 : ab218242 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 3 minutes.

The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.

The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID : 12767059; PMID : 20736367).

All lanes:

Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)

Predicted band size: 70 kDa,85 kDa,91 kDa

Observed band size: 100 kDa,75 kDa

false

Supplier Data

Western blot - Anti-ADAM9 antibody [EPR21924-164] (AB218242)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure times :
Lanes 1-5 : 3 minutes
Lane 6 : 48 seconds
Lane 7 : 114 seconds

The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID : 12767059; PMID : 20736367).

We recommend that customers avoid boiling their samples to prevent protein aggregation.

All lanes:

Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

WI-38 (human fetal lung fibroblast), whole cell lysate at 10 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 5:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 10 µg

Lane 6:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 7:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 91 kDa

Observed band size: 100 kDa,75 kDa

false

Lab

Western blot - Anti-ADAM9 antibody [EPR21924-164] (AB218242)

Lanes 1-3 : Merged signal (red and green). Green - ab218242 observed at 100,75 kDa. Red - loading control ab8245 observed at 36 kDa.

ab218242 Anti-ADAM9 antibody [EPR21924-164] was shown to specifically react with ADAM9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265484 (knockout cell lysate ab257814) was used. Wild-type and ADAM9 knockout samples were subjected to SDS-PAGE. ab218242 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ADAM9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ADAM9 knockout HeLa cell line (<a href='/products/cell-lines/human-adam9-knockout-hela-cell-line-ab265484'>ab265484</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 91 kDa

Observed band size: 100 kDa,75 kDa

false

不同偶联物与剂型 (2)

805 Alexa Fluor® 790

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed
Biotin

Goat Anti-Rabbit IgG H&L (Biotin)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21924-164

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody may not be suitable for tissue lysate testing.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ADAM9 also known as Meltrin gamma is a member of the ADAM (a disintegrin and metalloproteinase) family characterized by both proteolytic and adhesive properties. The ADAM9 protein weighs approximately 84 kDa and is expressed in various tissues including the brain throat and lungs. It plays an important role in cellular processes such as cell-cell and cell-matrix interactions due to its disintegrin domain.

Biological function summary

ADAM9 participates in numerous essential cellular functions particularly in cell surface protein shedding and proteolytic activities. It is part of the protease complex involved in shedding epidermal growth factor receptor (EGFR) ligands. This action impacts cell signaling pathways that contribute to processes such as cell proliferation migration and invasion.

Pathways

Many studies link ADAM9 to pathways involving cell signal modulation and extracellular matrix remodeling. It participates in the EGFR signaling pathway significantly affecting cell behavior. ADAM9 interacts with other proteins such as integrins which facilitate changes in cell dynamics and communication.

Evidence implicates ADAM9 in several cancers including breast cancer and prostate cancer. It plays a role in cancer development by promoting cell invasion and metastasis. ADAM9 is also associated with Alzheimer's disease through interactions with proteins like APP (amyloid precursor protein) suggesting its involvement in neuronal degradation and plaque formation.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Metalloprotease that cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as TEK, KDR, EPHB4, CD40, VCAM1 and CDH5. May mediate cell-cell, cell-matrix interactions and regulate the motility of cells via interactions with integrins.. Isoform 2. May act as alpha-secretase for amyloid precursor protein (APP).

See full target information ADAM9

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 56: PubMed40999989

2025

Cynarin as a potent anti‑osteolytic agent: Targeting MAPK and Nrf2‑Keap1 pathways for osteoclast inhibition and bone protection.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Chao,Yexin Wang,Zhan Liu,Tianhao Wan,Yi Mao,Xinru Xie,Lei Sun,Weifeng Xu,Xuzhuo Chen,Shanyong Zhang

International journal of medical sciences 22:630-640 PubMed39898241

2025

NACC1 accelerates the progression of AML by regulating the ADAM9/PI3K/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Liang Zhong,Peng Wan,Yi Zhao,Meng Wang,Hongyan Zhang,Yang Liao,Ying Deng,Beizhong Liu

Science translational medicine 14:eabj7521 PubMed34698500

2022

Identification of driver genes for critical forms of COVID-19 in a deeply phenotyped young patient cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Raphael Carapito,Richard Li,Julie Helms,Christine Carapito,Sharvari Gujja,Véronique Rolli,Raony Guimaraes,Jose Malagon-Lopez,Perrine Spinnhirny,Alexandre Lederle,Razieh Mohseninia,Aurélie Hirschler,Leslie Muller,Paul Bastard,Adrian Gervais,Qian Zhang,François Danion,Yvon Ruch,Maleka Schenck,Olivier Collange,Thiên-Nga Chamaraux-Tran,Anne Molitor,Angélique Pichot,Alice Bernard,Ouria Tahar,Sabrina Bibi-Triki,Haiguo Wu,Nicodème Paul,Sylvain Mayeur,Annabel Larnicol,Géraldine Laumond,Julia Frappier,Sylvie Schmidt,Antoine Hanauer,Cécile Macquin,Tristan Stemmelen,Michael Simons,Xavier Mariette,Olivier Hermine,Samira Fafi-Kremer,Bernard Goichot,Bernard Drenou,Khaldoun Kuteifan,Julien Pottecher,Paul-Michel Mertes,Shweta Kailasan,M Javad Aman,Elisa Pin,Peter Nilsson,Anne Thomas,Alain Viari,Damien Sanlaville,Francis Schneider,Jean Sibilia,Pierre-Louis Tharaux,Jean-Laurent Casanova,Yves Hansmann,Daniel Lidar,Mirjana Radosavljevic,Jeffrey R Gulcher,Ferhat Meziani,Christiane Moog,Thomas W Chittenden,Seiamak Bahram

OncoTargets and therapy 14:2773-2787 PubMed33907420

2021

Depletion of hsa_circ_0000144 Suppresses Oxaliplatin Resistance of Gastric Cancer Cells by Regulating miR-502-5p/ADAM9 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haifeng Gao,Jiajia Xu,Fen Qiao,Liangjun Xue

American journal of translational research 12:5525-5537 PubMed33042435

2020

Tetraspanin CD9 interacts with α-secretase to enhance its oncogenic function in pancreatic cancer.

Applications