重组Anti-ADAM10抗体[EPR5622] (ab124695)
![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-503821-anti-adam10-antibody-epr5622-western-blot-wildtype-jurkat-adam10-knockout-jurkat-mcf7-u2-os.jpg "Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5622] to ADAM10
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-ADAM10抗体[EPR5622]
参阅全部 ADAM10 一抗 -
描述
兔单克隆抗体[EPR5622] to ADAM10 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human ADAM10 (C terminal). The exact sequence is proprietary.
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阳性对照
- Jurkat, RAW 264.7, U2OS, LnCaP and MCF-7 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.80 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5622 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124695于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
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| IP |
1/10 - 1/100.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa. |
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IP
1/10 - 1/100. |
靶标
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功能
Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-
-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. -
组织特异性
Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver. -
序列相似性
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Cell membrane. Endomembrane system. Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi. - Information by UniProt
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数据库链接
- Entrez Gene: 102 Human
- Entrez Gene: 11487 Mouse
- Entrez Gene: 29650 Rat
- Omim: 602192 Human
- SwissProt: O14672 Human
- SwissProt: O35598 Mouse
- SwissProt: Q10743 Rat
- Unigene: 578508 Human
see all -
别名
- A disintegrin and metalloprotease domain 10 antibody
- A disintegrin and metalloproteinase domain 10 antibody
- AD 10 antibody
see all
图片
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Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution
Lane 1 : Wild-type Jurkat cell lysate at 10 µg
Lane 2 : ADAM10 knockout Jurkat cell lysate at 10 µg
Lane 3 : MCF7 cell lysate at 20 µg
Lane 4 : U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 84 kDa
Observed band size: 95-120 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ADAM10 antibody [EPR5622] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124695 was shown to bind specifically to ADAM10. A band was observed at 95/120 kDa in wild-type Jurkat cell lysates with no signal observed at this size in ADAM10 knockout cell line. The band at 120 kDa is likely to be the precursor and the band at 90 kDa is likely to be the active form of ADAM10. To generate this image, wild-type and ADAM10 knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-280814-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: Empty lane
Lane 3: ADAM10 knockout HAP1 whole cell lysate (40 µg)
Lane 4: U20S whole cell lysate (40 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab124695 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124695 was shown to recognize ADAM10 when ADAM10 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAM10 knockout samples were subjected to SDS-PAGE. Ab124695 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-282779-anti-adam10-antibody-epr5622-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ADAM10 antibody [EPR5622] (ab124695)ab124695 (purified) at 1:30 dilution (2ug) immunoprecipitating ADAM-10 in LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug.
Lane 1 (input): LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab124695 & LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate 10ug
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab170952 in HeLa LNCaP (Human prostate carcinoma epithelial cell) whole cell lysateFor western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-282717-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.05 µg/ml (Purified)
Lane 1 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-282715-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 0.09 µg/ml (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : LNCaP (Human prostate carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 84 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-138473-anti-adam10-antibody-epr5622.jpg)
OI-RD Scanning - Anti-ADAM10 antibody [EPR5622] (ab124695)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-138474-anti-adam10-antibody-epr5622-western-blot.jpg)
Western blot - Anti-ADAM10 antibody [EPR5622] (ab124695)All lanes : Anti-ADAM10 antibody [EPR5622] (ab124695) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 84 kDa
84 kDa band is pro-ADAM10, smaller band at around 65 kDa is mature ADAM10 protein. -
![Anti-ADAM10 antibody [EPR5622] (ab124695)](https://www.abcam.cn/ps/products/124/ab124695/Images/ab124695-8-benefits-of-recombinant-antibodies.png)
Anti-ADAM10 antibody [EPR5622] (ab124695)
数据表及文件
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SDS download
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Datasheet download
文献 (42)
ab124695 被引用在 42 文献中.
- Sivakumar S et al. Basal cell adhesion molecule promotes metastasis-associated processes in ovarian cancer. Clin Transl Med 13:e1176 (2023). PubMed: 36647260
- Espinosa-Jiménez T et al. A novel rhein-huprine hybrid ameliorates disease-modifying properties in preclinical mice model of Alzheimer's disease exacerbated with high fat diet. Cell Biosci 13:52 (2023). PubMed: 36895036
- Baek H et al. Cyanidin-3-O-glucoside protects the brain and improves cognitive function in APPswe/PS1ΔE9 transgenic mice model. J Neuroinflammation 20:268 (2023). PubMed: 37978414
- Ma W & Hu J The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression. Mol Med 29:162 (2023). PubMed: 38041015
- Abd-Elrahman KS et al. A positive allosteric modulator for the muscarinic receptor (M1 mAChR) improves pathology and cognitive deficits in female APPswe/PSEN1ΔE9 mice. Br J Pharmacol 179:1769-1783 (2022). PubMed: 34820835