Anti-Activin Receptor Type IIB/ACVR2B抗体(ab272869)
Key features and details
- Rabbit polyclonal to Activin Receptor Type IIB/ACVR2B
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Activin Receptor Type IIB/ACVR2B抗体
参阅全部 Activin Receptor Type IIB/ACVR2B 一抗 -
描述
兔多克隆抗体to Activin Receptor Type IIB/ACVR2B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human Activin Receptor Type IIB/ACVR2B. The exact sequence is proprietary. Center region.
Database link: Q13705 -
阳性对照
- WB: U-87 MG, SK-N-SH and SK-N-AS whole cell lysate, Mouse cerebellum tissue extract. IHC-P: Mouse placenta tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272869于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for activin A, activin B and inhibin A. -
疾病相关
Defects in ACVR2B are the cause of visceral heterotaxy autosomal type 4 (HTX4) [MIM:613751]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy type 4 include dextrocardia, right aortic arch and a right-sided spleen, anomalies of the inferior and the superior vena cava, atrial ventricular canal defect with dextro-transposed great arteries, pulmonary stenosis, polysplenia and midline liver. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 93 Human
- Entrez Gene: 11481 Mouse
- Omim: 602730 Human
- SwissProt: Q13705 Human
- SwissProt: P27040 Mouse
- Unigene: 174273 Human
- Unigene: 390239 Mouse
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别名
- Activin A receptor type IIB antibody
- Activin receptor type 2B antibody
- Activin receptor type IIB antibody
see all
图片
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Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell lysate
Lane 4 : SK-N-AS (human neuroblastoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)Paraffin-embedded mouse placenta tissue stained for Activin Receptor Type IIB/ACVR2B using ab272869 at a 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869) at 1/1000 dilution + Mouse cerebellum tissue at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Western blot - Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869)All lanes : Anti-Activin Receptor Type IIB/ACVR2B antibody (ab272869) at 1/50000 dilution
Lane 1 : Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected (+) HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272869 被引用在 1 文献中.
- Pei T et al. Bacteroides plebeius improves muscle wasting in chronic kidney disease by modulating the gut-renal muscle axis. J Cell Mol Med 26:6066-6078 (2022). PubMed: 36458537