重组Anti-Actin Regulatory蛋白CAPG/MCP抗体[EPR13194] (ab181092)
![Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)](https://www.abcam.cn/ps/products/181/ab181092/Images/ab181092-393635-anti-actin-regulatory-protein-capgmcp-antibody-epr13194-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13194] to Actin Regulatory Protein CAPG/MCP
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Actin Regulatory蛋白CAPG/MCP抗体[EPR13194]
参阅全部 Actin Regulatory Protein CAPG/MCP 一抗 -
描述
兔单克隆抗体[EPR13194] to Actin Regulatory蛋白CAPG/MCP -
宿主
Rabbit -
特异性
ab181092 may have very weak cross-reactivity with unknown proteins.
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经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, U937 and THP1 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13194 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).
|
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| IP |
1/60.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa). |
|
IP
1/60. |
靶标
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功能
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. -
组织特异性
Macrophages and macrophage-like cells. -
序列相似性
Belongs to the villin/gelsolin family.
Contains 3 gelsolin-like repeats. -
翻译后修饰
The N-terminus is blocked. -
细胞定位
Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 822 Human
- Omim: 153615 Human
- SwissProt: P40121 Human
- Unigene: 516155 Human
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别名
- Actin capping protein GCAP39 antibody
- Actin regulatory protein CAP G antibody
- Actin regulatory protein CAP-G antibody
see all
图片
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Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CAPG knockout HeLa cell lysate
Lane 3 : THP-1 cell lysate
Lane 4 : U-937 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab181092 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181092 Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] was shown to specifically react with Actin Regulatory Protein CAPG/MCP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265766 (knockout cell lysate ab257378) was used. Wild-type and Actin Regulatory Protein CAPG/MCP knockout samples were subjected to SDS-PAGE. ab181092 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)](https://www.abcam.cn/ps/products/181/ab181092/Images/ab181092-213120-ab181092.jpg)
Immunoprecipitation - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)Western blot analysis of THP1 cell lysate immunoprecipitated using ab181092 at 1/60 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution. -
![Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)](https://www.abcam.cn/ps/products/181/ab181092/Images/ab181092-213119-ab181092WBb.jpg)
Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/10000 dilution + THP1 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)](https://www.abcam.cn/ps/products/181/ab181092/Images/ab181092-213117-ab181092WB.jpg)
Western blot - Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092) at 1/50000 dilution + U937 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
![Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)](https://www.abcam.cn/ps/products/181/ab181092/Images/ab181092-5-benefits-of-recombinant-antibodies.png)
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194] (ab181092)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab181092 被引用在 2 文献中.
- Ma Q et al. Super-enhancer-associated gene CAPG promotes AML progression. Commun Biol 6:622 (2023). PubMed: 37296281
- Wang Z et al. LncRNA LINC01134 Contributes to Radioresistance in Hepatocellular Carcinoma by Regulating DNA Damage Response via MAPK Signaling Pathway. Front Pharmacol 12:791889 (2021). PubMed: 35173610