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Tags & Cell Markers Subcellular Markers Cytoskeleton Actin

Anti-Actin抗体- Loading Control (ab1801)

  • Datasheet
  • SDS
Reviews (11)Q&A (30)References (206)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)

Key features and details

  • Rabbit polyclonal to Actin - Loading Control
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Actin antibody [EPR16769] (ab179467)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Actin抗体- Loading Control
    参阅全部 Actin 一抗
  • 描述

    兔多克隆抗体to Actin - Loading Control
  • 宿主

    Rabbit
  • 特异性

    This antibody recognises beta and gamma actin in Human samples. It probably also recognises all the other known forms of Human actin. This antibody detects a single clean band in Human, Mouse, Rat, Chicken and Drosophila samples. In Xenopus laevis a secondary band is detected at about 30kDa. We are unsure whether this is cross-reaction with another actin isoform or merely non-specific. In Cow a doublet is detected, which probably represents different forms of actin.
  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Chicken, Cow, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa whole cell lysate or mouse brain lysate. IHC-P - Human Colon FFPE tissue section
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Tropomyosin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • RIP
    • Isotype/Loading Controls
    • Loading Controls
    • Actin
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • RIP
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Actin protein (Tagged) (ab235861)
  • Related Products

    • Wiskostatin, N-WASP inhibitor (ab141085)
    • CK 666, Actin polymerization inhibitor (ab141231)
    • Jasplakinolide, Actin polymerization and stabilization inducer (ab141409)
    • Cytochalasin E, actin-polymerization inhibitor (ab141788)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1801于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (9)
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block in 5% BSA. Blocking in milk significantly reduces the signal.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Block in 5% BSA. Blocking in milk significantly reduces the signal.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC/IF.

靶标

  • 功能

    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关

    Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
    Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
    Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
  • 序列相似性

    Belongs to the actin family.
  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68133 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 421534 Chicken
    • Entrez Gene: 281592 Cow
    • Entrez Gene: 58 Human
    • Entrez Gene: 11459 Mouse
    • Entrez Gene: 29437 Rat
    • Entrez Gene: 407658 Zebrafish
    • Omim: 102610 Human
    • SwissProt: P68139 Chicken
    • SwissProt: P68138 Cow
    • SwissProt: P68133 Human
    • SwissProt: P68134 Mouse
    • SwissProt: P68135 Rabbit
    • SwissProt: P68136 Rat
    • Unigene: 1288 Human
    • Unigene: 214950 Mouse
    • Unigene: 82732 Rat
    see all
  • 别名

    • a actin antibody
    • a-actin antibody
    • ACTA antibody
    • ACTA1 antibody
    • Actin alpha skeletal muscle antibody
    • Actin antibody
    • actin, alpha 1, skeletal muscle 1 antibody
    • actin, alpha 1, skeletal muscle antibody
    • Actin, alpha skeletal muscle antibody
    • actina antibody
    • actine antibody
    • ACTS_HUMAN antibody
    • aktin antibody
    • Alpha Actin 1 antibody
    • Alpha skeletal muscle Actin antibody
    • alpha skeletal muscle antibody
    • alpha-actin antibody
    • Alpha-actin-1 antibody
    • ASMA antibody
    • CFTD antibody
    • CFTD1 antibody
    • CFTDM antibody
    • MPFD antibody
    • NEM1 antibody
    • NEM2 antibody
    • NEM3 antibody
    • nemaline myopathy type 3 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Actin antibody - Loading Control (ab1801)

    IHC image of ab1801 staining Actin in normal human colon formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1801, 5μl/ml concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
    Western blot - Anti-Actin antibody - Loading Control (ab1801)
    All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate
    Lane 4 : NIH/3T3 whole cell lysate (ab7179)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1801 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406

  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
    Western blot - Anti-Actin antibody - Loading Control (ab1801)Image from PLoS One. 2014; 9(3): e92128. Fig 9,•DOI: 10.1371/journal.pone.0092128 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot analysis of HeLa cells treated for 12 hours with hesperidin (h) (2.5 μg/ml, 4,01 μM), mangiferin (5 μg/ml, 11.84 μM) (m), and hesperidin (2.5 μg/ml, 4.01 μM) in a presence of mangiferin (5 μg/ml, 11.84 μM) (h+m). Immunoblotting was performed with the following primary antibodies: Bax (ab32503), BCL2 (ab59348), beta actin (ab1801), and caspase 8. After the washing steps, the membranes were incubated with goat anti-rabbit IgG (H+L) or with goat anti-mouse IgG (H+L) HRP-conjugated secondary antibodies and detected using ECL. Densitometry was performed using Image Lab software v. 4.1 (BioRad).

    Top panel: Following 12h of treatment of HeLa cells with hesperidin (h), mangiferin (m), and hesperidin in a presence of mangiferin (h+m), the mRNA levels were monitored in real - time PCR experiments. The BAX and BCL2 mRNA levels results from 2 independent experiments (n?=?8) are plotted relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 18S rRNA levels and expressed as a fold change over the EtOH control. Error bars represent standard derivations.

    Bottom panel: Following 12h of treatment of HeLa cells with hesperidin (h), mangiferin (m), and hesperidin in a presence of mangiferin (h+m), the protein levels of Bax and BCL2 were detected with SDS-PAGE and Western Blot and related to beta actin levels.

  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
    Western blot - Anti-Actin antibody - Loading Control (ab1801)
    All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate (ab27253)
    Lane 2 : NIH/3T3 (Mouse) Whole Cell Lysate (ab52956)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa


    Exposure time: 30 seconds
  • Western blot - Anti-Actin antibody - Loading Control (ab1801)
    Western blot - Anti-Actin antibody - Loading Control (ab1801)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1/1000 dilution

    All lanes : Whole cell lysates prepared from HUVEC cells

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat polyclonal to rabbit Ig at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa


    Exposure time: 30 seconds

    See Abreview

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (206)

发表研究结果有使用 ab1801?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1801 被引用在 206 文献中.

  • Aleo SJ  et al. Drug repositioning as a therapeutic strategy for neurodegenerations associated with OPA1 mutations. Hum Mol Genet 29:3631-3645 (2021). PubMed: 33231680
  • Li X  et al. NR2F1-AS1/miR-140/HK2 Axis Regulates Hypoxia-Induced Glycolysis and Migration in Hepatocellular Carcinoma. Cancer Manag Res 13:427-437 (2021). PubMed: 33488124
  • Ruiz de Azua I  et al. Cannabinoid CB1 receptor in dorsal telencephalic glutamatergic neurons drives overconsumption of palatable food and obesity. Neuropsychopharmacology 46:982-991 (2021). PubMed: 33558679
  • Wyckelsma VL  et al. Loss of a-actinin-3 during human evolution provides superior cold resilience and muscle heat generation. Am J Hum Genet 108:446-457 (2021). PubMed: 33600773
  • Khalil MI  et al. NEK1 Phosphorylation of YAP Promotes Its Stabilization and Transcriptional Output. Cancers (Basel) 12:N/A (2020). PubMed: 33297404
View all Publications for this product

客户评价及客户问答

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提交评价 提交问题

1-10 of 41 Abreviews or Q&A

Western blot abreview for Anti-Actin antibody - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (cancer cell lines)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
15 µg
Specification
cancer cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 03 2019

Western blot abreview for Anti-Actin antibody - Loading Control

Poor
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (RIPA lysate of whole lung tissue)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
100 µg
Specification
RIPA lysate of whole lung tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ben Thomson

Verified customer

提交于 Aug 14 2015

Western blot abreview for Anti-Actin antibody - Loading Control

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 21 2014

Immunocytochemistry/ Immunofluorescence

Average
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cell (COS7)
Specification
COS7
Permeabilization
Yes - Triton X 100 0.3%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 03 2013

Question


Sorry it has taken me so long to respond to your email. We have tested the antibody in HeLa cell and no actin bands were detected. We have used these cells previously in other experiments, so we expect them to work with the Western.
We would greatly appreciate a refund for the product (ab1801).
Thank you,

Read More

Abcam community

Verified customer

Asked on Nov 15 2012

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. Since this order was paid for by credit card, the refund will go back onto the card. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Abcam Scientific Support

回复于 Nov 15 2012

Question

We ordered anti-actin rabbit polyclonal antibody (ab1801, ordered 8/31/12) and have not been able to get the antibody
to detect actin in a Western blot. We have followed the instructions for the antibody, which includes blocking in 5%
BSA and using a 1/1000 dilution. We have tried not only BSA, but 5% milk (even though the instructions state that milk reduces the signal). We have also tried a 1/500 dilution for the antibody. We have checked that the protein has transferred well using PonceauS stain. We have tried the Western using different anti-actin antibody as a control and have been able to detect actin. We have tried the Western with ab1801 several times with no luck.
Do you have other suggestions for the antibody? Would it be possible to get a refund or replacement for this antibody?
It is quite frustrating to do a Western blot where the experiment seems to be working, but our results are inconclusive because our actin control is not working.

Read More

Abcam community

Verified customer

Asked on Oct 25 2012

Answer

Thank you for taking time to contact us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Could I just ask to confirm the species that your samples were from (i.e. mouse)?

As long as the antibody was being used in a tested species, I would be happy to offer you a replacment or refund. Please let me know which you would prefer.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

回复于 Oct 25 2012

Question

I have used ab1801 to blot for actin from human whole cell extracts and seen no bands; stripping and reprobing with another anti-actin antibody provides expected results;

Read More

Abcam community

Verified customer

Asked on Aug 27 2012

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab8227.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

回复于 Aug 27 2012

Question

Last month I order Anti-Actin antibody - Loading Control (ab1801) from your company.

I ran the western blot assays with this antibody (rabbit, 1:1000) and used anti-rabbit secondary antibody (1:1000), but I get the white bands of actin (light black background)and dark black bands of molecular markersin the X-tay films. At the same time, I use the anti-tubulin (cell signaling) with the same procedures andget the normal balck bands oftubulinin the X-ray films.

Could you tell me what is the problem?

Read More

Abcam community

Verified customer

Asked on Aug 17 2012

Answer

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

It sounds like you're seeing "inverse banding" which is white bands on a black background. Usually this means that you're using too much primary and/or secondary antibody. I'd recommend diluting the primary to 1:2000 and incubating this overnight at 4C in the blocking buffer (5% BSA) and using the secondary at 1:5000 - 10,000 for 1 hr at RT in blocking buffer. A 1:1000 dilution for a secondary antibody is quite high.

Are you blocking in 5% BSA?

How much protein are you loading and what are your samples (species, cell line, etc.)?

Do you have an image that you can send?


I would strongly recommend that you try diluting the primary and secondary more. Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Read More

Abcam Scientific Support

回复于 Aug 17 2012

Question


Could you please provide us a testing code for ab121733?
Thank you very much for all your support.

Read More

Abcam community

Verified customer

Asked on Aug 14 2012

Answer

Thank you very much for your reply.

Please see below for the testing code to try ab121733 with zebrafish samples:

DISCOUNT CODE: ***
EXPIRATION DATE: December 12, 2012

I do hope that this antibody will work well with zebrafish, but if you need any assitance optimizing the protocol or anything else, please let me know and I'll be happy to help.

Have a nice day!

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Abcam Scientific Support

回复于 Aug 14 2012

Question


Thank you for your reply. We are wondering if it is possible to use
testing code for replacing anti-WDR81 primary antibody with another
antibody (since there is no another Abcam WDR81 antibodies to compare it
to). I am asking this in order to make sure the conditions of using
testing code in case ab121733 does not react with zebrafish samples even
with higher concentrations.
Have a nice weekend.
Best,

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Abcam community

Verified customer

Asked on Aug 13 2012

Answer

Thank you very much for your reply.

The testing codes can actuallybe used to receive any primary antibody in our catalog, regardless of target. So, if the WDR81 antibody does not work with zebrafish samples, you can use the code to get any other primary antibody of your choosing.

I hope that this information will be useful, but please let me know if you have further questions. Have a nice day!

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Abcam Scientific Support

回复于 Aug 13 2012

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