Anti-ACT抗体(ab97396)
Key features and details
- Rabbit polyclonal to ACT
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ACT抗体 -
描述
兔多克隆抗体to ACT -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 and 196 of human ACT (NP_065228)
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阳性对照
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and Raji cells. SW480 xenograft.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97396于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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| IHC-P |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
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IHC-P
1/100 - 1/500. |
靶标
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功能
May be involved in the regulation of spermatogenesis. Stimulates CREM transcriptional activity in a phosphorylation-independent manner. -
组织特异性
Testis-specific (at protein level). -
序列相似性
Contains 4 LIM zinc-binding domains. -
细胞定位
Nucleus. Nuclei of round and elongated spermatids. - Information by UniProt
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数据库链接
- Entrez Gene: 9457 Human
- Entrez Gene: 57756 Mouse
- Entrez Gene: 297954 Rat
- Omim: 605126 Human
- SwissProt: Q5TD97 Human
- SwissProt: Q8WW21 Human
- SwissProt: Q9WTX7 Mouse
- SwissProt: Q6AXT1 Rat
see all -
别名
- Activator of cAMP responsive element modulator (CREM) in testis antibody
- Activator of cAMP-responsive element modulator in testis antibody
- Activator of CREM in testis antibody
see all
图片
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Western blot - Anti-ACT antibody (ab97396)All lanes : Anti-ACT antibody (ab97396) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
Gel conc 12 % (SDS PAGE) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACT antibody (ab97396)ab97396 at 1/500 dilution, staining human ACT in SW480 xenograft - Formalin/PFA-fixed paraffin-embedded tissue.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab97396 被引用在 1 文献中.
- Jazireian P et al. Dynamic Expression and Chromatin Incorporation of ACT and CREM Transcription Factors in Testis Tissues of Infertile Men. Cell J 23:736-741 (2021). PubMed: 34979062